TITLE

CARACTERIZACIÓN MOLECULAR DE AISLADOS DE Listeria monocytogenes MEDIANTE ERIC-PCR

AUTHOR(S)
Isabel, Tapia V.; Eugenio, Reyes A.; Andrea, Lucero V.; Patricia, González A.; Sara, Morales R.; Aldo, Mori A.; José, Sendra D.; Melissa, Puentes S.; Christian, Verdugo E.
PUB. DATE
December 2012
SOURCE
Revista Chilena de Tecnología Médica;2012, Vol. 32 Issue 2, p1707
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Listeria monocytogenes is an emergent pathogen which is transmitted by consuming poisoned food causing illness in human beings and animals. There are several methods available for typing strains of Listeria monocytogenes. The traditional methods have the disvantage of long diagnosis timing, need different reactive and mediums and the possibility of false positives and negatives. RAPD had been used for epidemiologic purpose in development countries because its high discriminatory power. Although, there are no publication using ERIC primers for the characterization of Listeria monocytogenes in Chile. This study characterize isolated strains of Listeria monocytogenes of the 2008 outbreak associated to 009 clone and meat isolated strains, using ERIC-1 (R) y ERIC-2 primers, obtaining 1 to 5 bands between 100 and 100 bp depending of the analyzed strain, showing through dendograms its capacity of discriminate isolates from clinical and food samples.
ACCESSION #
86411032

 

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