TITLE

The enhancement of biofilm formation in Group B streptococcal isolates at vaginal pH

AUTHOR(S)
Ho, Yueh-Ren; Li, Chien-Ming; Yu, Chen-Hsiang; Lin, Yuh-Jyh; Wu, Ching-Ming; Harn, I-Chen; Tang, Ming-Jer; Chen, Yi-Ting; Shen, Fang-Chi; Lu, Chien-Yi; Tsai, Tai-Chun; Wu, Jiunn-Jong
PUB. DATE
April 2013
SOURCE
Medical Microbiology & Immunology;Apr2013, Vol. 202 Issue 2, p105
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Group B streptococcus (GBS) is a common asymptomatic colonizer in acidic vagina of pregnant women and can transmit to newborns, causing neonatal pneumonia and meningitis. Biofilm formation is often associated with bacterial colonization and pathogenesis. Little is known about GBS biofilm and the effect of environmental stimuli on their growth along with biofilm formation. The objective of this study was to investigate the survival and biofilm formation of GBS, isolated from pregnant women, in nutrient-limited medium under various pH conditions. Growth and survival experiments were determined by optical density and viable counts. Crystal violet staining, scanning electron microscopy, and atomic force microscopy (AFM) were used to analyze the capacity of biofilm production. Our results showed that GBS isolates proliferated with increasing pH with highest maximum specific growth rate (μ) at pH 6.5, but survived at pH 4.5 for longer than 48 h. Biofilm formation of the 80 GBS isolates at pH 4.5 was significantly higher than at pH 7.0. This difference was confirmed by two other methods. The low elastic modulus obtained from samples at pH 4.5 by AFM revealed the softness of biofilm; in contrast, little or no biofilm was measured at pH 7.0. Under acidic pH, the capability of biofilm formation of serotypes III and V showed statistically significant difference from serotypes Ia and Ib. Our finding suggested that survival and enhanced biofilm formation at vaginal pH are potentially advantageous for GBS in colonizing vagina and increase the risk of vaginosis and neonatal infection.
ACCESSION #
86406719

 

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics