Expression and clinical significance of microRNA-326 in human glioma miR-326 expression in glioma

Wang, Shuai; Lu, Shengkui; Geng, Shaomei; Ma, Shucheng; Liang, Zhaohui; Jiao, Baohua
March 2013
Medical Oncology;Mar2013, Vol. 30 Issue 1, p1
Academic Journal
As a suppressor of Hedgehog signaling pathway, microRNA-326 (miR-326) has been demonstrated to control the development of cerebellar neuronal progenitor and tumor cells. More recently, it has been reported that miR-326 was down-regulated in glioblastoma tissues and might regulate the metabolic activity of glioma and glioma stem cells, suggesting the involvement of miR-326 in tumorigenesis and progression of gliomas. However, the role of miR-326 in human glioma has not been clearly understood. Therefore, the aim of this study was to investigate the clinical significance of miR-326 expression in human glioma. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to characterize the expression patterns of miR-326 in 108 glioma and 20 normal brain tissues. The associations of miR-326 expression with clinicopathological factors and prognosis of glioma patients were also statistically analyzed. The expression levels of miR-326 in glioma tissues were significantly lower than those in normal brain tissues ( P < 0.001). Additionally, the decreased miR-326 expression in glioma was significantly associated with advanced pathological grade ( P = 0.01) and low Karnofsky performance score (KPS, P = 0.03). Moreover, Kaplan-Meier survival and Cox regression analyses showed that low expression of miR-326 ( P = 0.01) and advanced pathological grade ( P = 0.02) were independent factors predicting poor prognosis for gliomas. Furthermore, subgroup analyses showed that miR-326 expression was significantly associated with poor overall survival in glioma patients with high pathological grades (for grade III-IV: P < 0.001). Down-regulation of miR-326 may have potential value for predicting clinical outcomes in glioma patients with high pathological grades, suggesting that miR-326 is an important candidate tumor suppressor, and its down-regulated expression may contribute to glioma progression.


Related Articles

  • Hedgehog signaling pathway: Epigenetic regulation and role in disease and cancer development. Fattahi, Sadegh; Pilehchian Langroudi, Maryam; Akhavan‐Niaki, Haleh // Journal of Cellular Physiology;Aug2018, Vol. 233 Issue 8, p5726 

    The evolutionarily conserved Hedgehog (Hh) signaling pathway have critical roles in development and homeostasis of tissues. Under physiological conditions, Hh is controlled at different levels via stem cell maintenance and tissue regeneration. Aberrant activation of this signaling pathway may...

  • Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway. GUANG-NING YAN; YANG-FAN LV; LANG YANG; XIAO-HONG YAO; YOU-HONG CUI; DE-YU GUO // Oncology Letters;2013, Vol. 6 Issue 5, p1524 

    The aim of the present study was to determine the possible mechanism underlying the enhanced migration and proliferation of endothelial cells caused by glioma stem cells (GSCs). Tumor spheres enriched in GSCs derived from the mouse GL261 glioma cell line, and the brain microvessel endothelial...

  • Hedgehog Signaling is Associated with Liver Response to Fractionated Irradiation in Mice. Kim, Jieun; Wang, Sihyung; Hyun, Jeongeun; Guy, Cynthia D.; Jung, Youngmi // Cellular Physiology & Biochemistry (Karger AG);Nov2016, Vol. 40 Issue 1/2, p263 

    Background/Aims: Radiation-induced liver disease (RILD) is a major obstacle in treating liver cancer; however, the mechanisms underlying RILD development remain unclear. Hedgehog (Hh) orchestrates liver response to injury. Herein, we investigated the liver response with Hh to fractionated...

  • MicroRNA-182 promotes leptomeningeal spread of non-sonic hedgehog-medulloblastoma. Bai, Alfa; Milde, Till; Remke, Marc; Rolli, Claudio; Hielscher, Thomas; Cho, Yoon-Jae; Kool, Marcel; Northcott, Paul; Jugold, Manfred; Bazhin, Alexandr; Eichmüller, Stefan; Kulozik, Andreas; Pscherer, Armin; Benner, Axel; Taylor, Michael; Pomeroy, Scott; Kemkemer, Ralf; Witt, Olaf; Korshunov, Andrey; Lichter, Peter // Acta Neuropathologica;Apr2012, Vol. 123 Issue 4, p529 

    The contribution of microRNAs to the initiation, progression, and metastasis of medulloblastoma (MB) remains poorly understood. Metastatic dissemination at diagnosis is present in about 30% of MB patients, and is associated with a dismal prognosis. Using microRNA expression profiling, we...

  • Molecular analysis of ex-vivo CD133+ GBM cells revealed a common invasive and angiogenic profile but different proliferative signatures among high grade gliomas. Garcia, Juan L.; Perez-Caro, Maria; Gomez-Moreta, Juan A.; Gonzalez, Francisco; Ortiz, Javier; Blanco, Oscar; Sancho, Magdalena; Hernandez-Rivas, Jesus M.; Gonzalez-Sarmiento, Rogelio; Sanchez-Martin, Manuel // BMC Cancer;2010, Vol. 10, p454 

    Background: Gliomas are the most common type of primary brain tumours, and in this group glioblastomas (GBMs) are the higher-grade gliomas with fast progression and unfortunate prognosis. Two major aspects of glioma biology that contributes to its awful prognosis are the formation of new blood...

  • MicroRNA Expression and Association with Clinicopathologic Features in Papillary Thyroid Cancer: A Systematic Review. Aragon Han, Patricia; Weng, Chien-Hsiang; Khawaja, Hunain T.; Nagarajan, Neeraja; Schneider, Eric B.; Umbricht, Christopher B.; Witwer, Kenneth W.; Zeiger, Martha A. // Thyroid;Dec2015, Vol. 25 Issue 12, p1322 

    Background: Studies have suggested that microRNAs (miR) may be useful prognostic markers and are associated with aggressive clinicopathologic features in papillary thyroid cancer (PTC). This systematic review examined associations between miRs and aggressive clinicopathologic features in PTC....

  • Aberrant expression of miR-199a-3p and its clinical significance in colorectal cancers. Wan, Daiwei; He, Songbing; Xie, Binhui; Xu, Guohui; Gu, Wen; Shen, Chenglong; Hu, You; Wang, Xinsheng; Zhi, Qiaoming; Wang, Liang // Medical Oncology;Mar2013, Vol. 30 Issue 1, p1 

    Aberrant miR-199a-3p expression has been reported in several cancers. However, the clinical significance of miR-199a-3p in human colorectal cancer has not been addressed. In this study, we detected miR-199a-3p expression in 92 colorectal cancer cases to evaluate its clinicopathologic...

  • Prognostic significance of serum miR-17-5p in lung cancer. Chen, Qun; Si, Qing; Xiao, Song; Xie, Qiang; Lin, Jiangping; Wang, Chenhui; Chen, Lizhou; Chen, Qiaolin; Wang, Lin // Medical Oncology;Mar2013, Vol. 30 Issue 1, p1 

    miR-17-5p is abnormally expressed in various tumor types. The aim of this study was to investigate the expression level of miR-17-5p in serum of patients with lung cancer and to determine whether serum miR-17-5p expression is related to the prognosis of patients with lung cancer. RT-qPCR was...

  • Identification of featured biomarkers in breast cancer with microRNA microarray. Zhang, Ming; Liu, Dequan; Li, Wenhui; Wu, Xiaoli; Gao, Chang'e; Li, Xiangnan // Archives of Gynecology & Obstetrics;Nov2016, Vol. 294 Issue 5, p1047 

    Purpose: We aimed to screen possible biomarkers associated with the molecular mechanism of breast cancer using microRNA (miRNA) microarray.Methods: The miRNAs expression profile GSE45666 was downloaded from Gene Expression Omnibus database, which included 101 genechips...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics