TITLE

Post-analysis follow-up and validation of microarray experiments

AUTHOR(S)
Chuaqui, Rodrigo F.; Bonner, Robert F.; Best, Carolyn J.M.; Gillespie, John W.; Flaig, Michael J.; Hewitt, Stephen M.; Phillips, John L.; Krizman, David B.; Tangrea, Michael A.; Ahram, Mamoun; Linehan, W. Marston; Knezevic, Vladimir; Emmert-Buck, Michael R.
PUB. DATE
December 2002
SOURCE
Nature Genetics;Dec2002 Supplement 2, Vol. 32, p509
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Measurement of gene-expression profiles using microarray technology is becoming increasingly popular among the biomedical research community. Although there has been great progress in this field, investigators are still confronted with a difficult question after completing their experiments: how to validate the large data sets that are generated? This review summarizes current approaches to verifying global expression results, discusses the caveats that must be considered, and describes some methods that are being developed to address outstanding problems.
ACCESSION #
8584150

 

Related Articles

  • Navigating gene expression using microarrays ? a technology review. Schulze, Almut; Downward, Julian // Nature Cell Biology;Aug2001, Vol. 3 Issue 8, pE190 

    Parallel quantification of large numbers of messenger RNA transcripts using microarray technology promises to provide detailed insight into cellular processes involved in the regulation of gene expression. This should allow new understanding of signalling networks that operate in the cell and of...

  • Microarray-based analyses of mRNA decay in the regulation of mammalian gene expression. Raghavan, Arvind; Bohjanen, Paul R. // Briefings in Functional Genomics & Proteomics;Aug2004, Vol. 3 Issue 2, p112 

    Mammalian cells coordinately regulate their gene expression programmes to ensure appropriate responses to stimuli. While transcriptional events provide an important level of gene expression regulation, modulation of messenger RNA (mRNA) decay provides an additional critical regulatory step. Much...

  • Do DNA Microarrays Tell the Story of Gene Expression? Rosenfeld, Simon // Gene Regulation & Systems Biology;2010, Issue 4, p61 

    Poor reproducibility of microarray measurements is a major obstacle to their application as an instrument for clinical diagnostics. In this paper, several aspects of poor reproducibility are analyzed. All of them belong to the category of interpretive weaknesses of DNA microarray technology....

  • Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance. Nygaard, Vigdis; Løland, Anders; Holden, Marit; Langaas, Mette; Rue, Håvard; Fang Liu; Myklebost, Ola; Fodstad, Øystein; Hovig, Eivind; Smith-Sørensen, Birgitte // BMC Genomics;2003, Vol. 4, p11 

    Background: A limiting factor of cDNA microarray technology is the need for a substantial amount of RNA per labeling reaction. Thus, 20-200 micro-grams total RNA or 0.5-2 micro-grams poly (A) RNA is typically required for monitoring gene expression. In addition, gene expression profiles from...

  • Selecting informative genes for discriminant analysis using multigene expression profiles. Xin Yan; Tian Zheng // BMC Genomics;2008 Supplement 2, Vol. 9, Special section p1 

    Background: Gene expression data extracted from microarray experiments have been used to study the difference between mRNA abundance of genes under different conditions. In one of such experiments, thousands of genes are measured simultaneously, which provides a high-dimensional feature space...

  • Gene Expression in the Cyanobacterium Anabaena sp. PCC7120 under Desiccation. Katoh, H.; Asthana, R. K.; Ohmori, M. // Microbial Ecology;Feb2004, Vol. 47 Issue 2, p164 

    The N2-fixing cyanobacterium Anabaena sp. PCC7120 showed an inherent capacity for desiccation tolerance. A DNA microarray covering almost the entire genome of Anabaena was used to determine the genome-wide gene expression under desiccation. RNA was extracted from cells at intervals starting from...

  • Expression Quantitative Trait Loci Analysis of 13 Genes in the Rat Prostate. Yamashita, Satoshi; Wakazono, Kuniko; Nomoto, Tomoko; Tsujino, Yoshimi; Kuramoto, Takashi; Ushijima, Toshikazu // Genetics;Nov2005, Vol. 171 Issue 3, p1231 

    Differential expression of mRNA among animal strains is one of the mechanisms for their diversity. cDNA microarray analysis of the prostates of BUF/Nac (BUF) and ACI/N (ACI) rats, which show different susceptibility to prostate cancers, found 195 differentially expressed genes. To identify loci...

  • Spontaneous Immortalization of Human Epidermal Cells with Naturally Elevated Telomerase. Rea, Miguel A.; Lei Zhou; Qin Qin; Barrandon, Yann; Easley, Karen W.; Gungner, Sarah F.; Phillips, Marjorie A.; Holland, William S.; Gumerlock, Paul H.; Rocke, David M.; Rice, Robert H. // Journal of Investigative Dermatology;Nov2006, Vol. 126 Issue 11, p2507 

    This work explores spontaneous immortalization in keratinocytes, derived from two skin samples, that display naturally elevated telomerase activity. Serially passaged with 3T3 feeder layer support, the keratinocytes were examined for colony-forming ability, telomerase activity, telomere length,...

  • Evaluation of five different cDNA labeling methods for microarrays using spike controls. Badiee, Azadeh; Eiken, Hans Geir; Steen, Vidar M.; Løvlie, Roger // BMC Biotechnology;2003, Vol. 3, p23 

    Background: Several different cDNA labeling methods have been developed for microarray based gene expression analysis. We have examined the accuracy and reproducibility of such five commercially available methods in detection of predetermined ratio values from target spike mRNAs (A. thaliana) in...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics