TITLE

Application of fluorescent dyes for falsification-preventing of pea seeds (Pisum sativum L.)

AUTHOR(S)
Yixin Tian; Qitian Wang; Jin Hu; Qijuan Hu; Jiancheng Wang; Yajing Guan
PUB. DATE
January 2013
SOURCE
Australian Journal of Crop Science;2013, Vol. 7 Issue 1, p147
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In the present study, combining with the technology of seed coating, the fluorescent compounds of rhodamine B (RB) and safranine T (ST) were used to label seeds as an anti-counterfeiting technology. The experiment was performed to investigate the effects of coating seeds with fluorescent dyes on physiological parameters of pea and the fluorescence performance in pea seedlings. The results showed that fluorescent dyes had no negative effect on seed germination, seedling growth, seedling protective enzyme activities, malondialdehyde (MDA) and chlorophyll content after coating with mass ratio of 1Kg RB to 20∼30Kg pea seeds and 1 Kg ST to 10∼30Kg pea seeds. RB under fluorescence microscope emitted bright red fluorescence and bright orange fluorescence excited by green light (546 nm) and blue light (495 nm), respectively. The fluorescence intensity in RB was higher than that in ST. Moreover, the vascular bundles of stem, roots and aerial parts of seedlings treated with RB all emitted brilliant fluorescence for a long time, which could be used as a marker in seedlings. It suggests that pea seeds labeling with RB dye by coating way at appropriate proportion can be used as an anti-counterfeiting technique in pea seeds.
ACCESSION #
85668982

 

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