TITLE

Plasmalemmal concentration and affinity of mouse vascular endothelial cadherin, VE-cadherin

AUTHOR(S)
Baumgartner, Werner; Drenckhahn, Detlev
PUB. DATE
December 2002
SOURCE
European Biophysics Journal;2002, Vol. 31 Issue 7, p532
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Ca[sup 2+] -dependent adhesion molecules, cadherins, are critically involved in the barrier formation of epithelial layers. Adhesive strength depends on both the plasmalemmal concentration and adhesive affinity (affinity for trans interaction) of cadherins. In the present study we used recombinant vascular endothelial cadherin, VE-cadherin, as a reference to quantify the surface concentration of VE-cadherin in mouse microvascular endothelial cells by linear interpolation and regression analysis of immunosignals obtained with cell lysates dotted on nitrocellulose membranes. The affinity of trans interaction was determined by a novel mobility shift assay, in which soluble dimeric VE-cadherin ectodomains pass through a VE-cadherin affinity column. By these approaches we determined the trypsin-sensitive surface concentration of VE-cadherin to be 5×10[sup 3] dimers/µm[sup 2] cell surface and the dissociation constant K[sub D] to be about 0.8×10[sup –4] M. The low affinity of trans interaction in combination with high plasmalemmal concentration of VE-cadherins fulfils theoretical predictions for regulation of adhesion by a transmembrane cooperative linkage mechanism, in which the degree of lateral mobility (translational entropy) of cadherins in the plasma membrane determines the number of adhesive bonds and, hence, the strength of intercellular adhesion.
ACCESSION #
8563657

 

Related Articles

  • Flightless I Regulates Hemidesmosome Formation and Integrin-Mediated Cellular Adhesion and Migration during Wound Repair. Kopecki, Zlatko; Arkell, Ruth; Powell, Barry C.; Cowin, Allison J. // Journal of Investigative Dermatology;Aug2009, Vol. 129 Issue 8, p2031 

    Flightless I (Flii), a highly conserved member of the gelsolin family of actin-remodelling proteins associates with actin structures and is involved in cellular motility and adhesion. Our previous studies have shown that Flii is an important negative regulator of wound repair. Here, we show that...

  • The function of 7D-cadherins: a mathematical model predicts physiological importance for water transport through simple epithelia. Ahl, Mareike; Weth, Agnes; Walcher, Sebastian; Baumgartner, Werner // Theoretical Biology & Medical Modelling;2011, Vol. 8 Issue 1, p18 

    Background: 7D-cadherins like LI-cadherin are cell adhesion molecules and represent exceptional members of the cadherin superfamily. Although LI-cadherin was shown to act as a functional Ca2+-dependent adhesion molecule, linking neighboring cells together, and to be dysregulated in a variety of...

  • Cell biology of the neuron: Growth cones go rafting. Jones, Rachel // Nature Reviews Neuroscience;Feb2003, Vol. 4 Issue 2, p87 

    Presents insights into the regulation of growth cone motility by cell adhesion molecules (CAM). Localization of CAM to specialized microdomains within the cell membrane, known as lipid rafts; Description of lipid rafts; Regional requirements for detergent-resistant membranes.

  • Wound repair and anti-oxidative capacity is regulated by ITGB4 in airway epithelial cells. Liu, Chi; Liu, Hui-jun; Xiang, Yang; Tan, Yu-rong; Zhu, Xiao-lin; Qin, Xiao-qun // Molecular & Cellular Biochemistry;Aug2010, Vol. 341 Issue 1/2, p259 

    Integrin beta 4 (ITGB4) is a structural adhesion molecule which engages in maintaining the integrity of airway epithelial cells. Its specific cytomembrane structural feature strongly indicates that ITGB4 may engage in many signaling pathways and physiologic processes. However, in addition to...

  • Significance of EpCAM and TROP2 expression in non-small cell lung cancer.  // World Journal of Surgical Oncology;2012, Vol. 10 Issue 1, p53 

    The article offers information on a study conducted on the significance of epithelial cell adhesion molecule (EpCAM) and type 1 transmembrane protein (TROP2) expression and clinicopathologic significance in non-small cell lung cancer (NSCLC). It states that Pearson Chi-square test or Fisher's...

  • ICAM-2 and lipid rafts disappear from the basal plasma membrane of uterine epithelial cells during early pregnancy in rats. Lecce, L.; Lindsay, L.; Kaneko, Y.; Murphy, C. // Cell & Tissue Research;Sep2013, Vol. 353 Issue 3, p563 

    Adhesion molecules are redistributed in rat uterine epithelial cells (UECs) during early pregnancy for endometrial receptivity and implantation. Intercellular adhesion molecule-2 (ICAM-2) is located as an oligomer on the basal plasma membrane of non-receptive UECs on day 1 of pregnancy and...

  • Molecular Mechanisms Controlling Vascular Lumen Formation in Three-Dimensional Extracellular Matrices. Sacharidou, Anastasia; Stratman, Amber N.; Davis, George E. // Cells Tissues Organs;2011, Vol. 195 Issue 1/2, p122 

    Considerable progress has been made toward a molecular understanding of how cells form lumen and tube structures in three-dimensional (3D) extracellular matrices (ECM). This progress has occurred through work performed with endothelial and epithelial cell models using both in vitro and in vivo...

  • Intercellular adhesion molecule-1 expression on human corneal epithelial outgrowth from limbal explant in culture. Iwata, M.; Fushimi, N.; Suzuki, Y.; Sakimoto, T.; Sawa, M. // British Journal of Ophthalmology;Feb2003, Vol. 87 Issue 2, p203 

    Aim: To investigate the relation between intercellular adhesion molecule (ICAM)-1 expression and cellular dynamics occurring concomitantly with epithelial cell movement. Methods: Outgrowing epithelial sheets of human corneal epithelial (HCE) cells from cultured limbal explants were examined by...

  • Respiratory medicine.  // Clinical & Investigative Medicine;Aug97 Supplement, Vol. 20, pS100 

    Presents an abstract of the research manuscript `Effect of adenovirus E1A on the intercellular adhesion molecule-1 ptomotor in pumonary epithelial cells and bronchial epithelial cells,' by Y. Higashimoto et al.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics