TITLE

SIMULTANEOUS HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF IRBESARTAN AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICAL DOSAGE FORM

AUTHOR(S)
Ubale, Milind B.; Dhakane, Vitthal D.; Chaudhari, Vilas R.
PUB. DATE
January 2012
SOURCE
Journal of Pharmaceutical & Scientific Innovation;Jan/Feb2012, Vol. 1 Issue 1, p25
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A simple, rapid, accurate and precise method is developed for the quantitative simultaneous determination of Irbesartan and Hydrochlorothiazide in combined pharmaceutical dosage form. Separation is achieved with an Ace5- C18,( 250 X 4.6)mm,5μ analytical column using buffer-acetonitrile (65:35, v/v) of pH 5.5, adjusted with acetic acid as the mobile phase. The buffer used in mobile phase contains 50Mm ammonium acetate in double distilled water. The instrumental setting is flow rate of 1 mL/ min, column temperature at 30℃, and detector wavelength of 260 nm. The internal standard method is used for the quantitation of the ingredients of this combination. Methylparaben is used as an internal standard. The method is validated and shown to Hydrochlorothiazide are 0.9998 and 0.9999, respectively. The relative standard deviations for six replicate measurements in two sets of each drug in tablets are always less than 2%. be linear for Irbesartan and Hydrochlorothiazide. The correlation coefficient for Irbesartan and Hydrochlorothiazide are 0.9998 and 0.9999 respectively. The relative standard deviation for six replicate measurements in two sets of each drugs in tablets are always less than 2%.
ACCESSION #
85289575

 

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