TITLE

Extended-Spectrum β-Lactamase–Producing Escherichia coli From Retail Chicken Meat and Humans: Comparison of Strains, Plasmids, Resistance Genes, and Virulence Factors

AUTHOR(S)
Kluytmans, Jan A. J. W.; Overdevest, Ilse T. M. A.; Willemsen, Ina; Kluytmans-van den Bergh, Marjolein F. Q.; van der Zwaluw, Kim; Heck, Max; Rijnsburger, Martine; Vandenbroucke-Grauls, Christina M. J. E.; Savelkoul, Paul H. M.; Johnston, Brian D.; Gordon, David; Johnson, James R.
PUB. DATE
February 2013
SOURCE
Clinical Infectious Diseases;Feb2013, Vol. 56 Issue 4, p478
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We compared the genetic composition of extended-spectrum β-lactamase–producing Escherichia coli from chicken meat, human carriers, and blood cultures in the Netherlands. The substantial overlap found among the 3 groups indicates that chicken meat is a source for resistant E. coli in humans.Background. The worldwide prevalence of extended-spectrum β-lactamase (ESBL)–producing Enterobacteriaceae is increasing rapidly both in hospitals and in the community. A connection between ESBL-producing bacteria in food animals, retail meat, and humans has been suggested. We previously reported on the genetic composition of a collection of ESBL-producing Escherichia coli (ESBL-EC) from chicken meat and humans from a restricted geographic area. Now, we have extended the analysis with plasmid replicons, virulence factors, and highly discriminatory genomic profiling methods.Methods. One hundred forty-five ESBL-EC isolates from retail chicken meat, human rectal carriers, and blood cultures were analyzed using multilocus sequence typing, phylotyping, ESBL genes, plasmid replicons, virulence genes, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE).Results. Three source groups overlapped substantially when their genetic composition was compared. A combined analysis using all variables yielded the highest resolution (Wilks lambda [Λ]: 0.08). Still, a prediction model based on the combined data classified 40% of the human isolates as chicken meat isolates. AFLP and PFGE showed that the isolates from humans and chicken meat could not be segregated and identified 1 perfect match between humans and chicken meat.Conclusions. We found significant genetic similarities among ESBL-EC isolates from chicken meat and humans according to mobile resistance elements, virulence genes, and genomic backbone. Therefore, chicken meat is a likely contributor to the recent emergence of ESBL-EC in human infections in the study region. This raises serious food safety questions regarding the abundant presence of ESBL-EC in chicken meat.
ACCESSION #
85215059

 

Related Articles

  • ESBL-plasmids carrying toxin-antitoxin systems can be "cured" of wild-type Escherichia coli using a heat technique. Schaufler, Katharina; Wieler, Lothar H.; Semmler, Torsten; Ewers, Christa; Guenther, Sebastian // Gut Pathogens;2013, Vol. 5 Issue 1, p1 

    Background Plasmid-encoded extended-spectrum beta-lactamase (ESBL)-enzymes are frequently produced by Escherichia coli. Several ESBL-plasmids contain genes for toxin-antitoxin (TA) systems, which assure the maintenance of plasmids in bacteria and prevent the cells from "post-segregational...

  • Characterization of the extended-spectrum b-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children. Bedenić, Branka; Vraneš, Jasmina; Hofmann-Thiel, Sabine; Tonkić, Marija; Novak, Anita; Bučević-Popovic, Viljemka; Hoffmann, Harald // Wiener Klinische Wochenschrift;Aug2012, Vol. 124 Issue 15/16, p504 

    Background and aim: The aim of the study was to characterize ESBL-producing uropathogenic Escherichia coli (UPEC) strains isolated in children. That included the investigation of virulence factors and the analysis of the types of β-lactamases at the molecular genetic level. Material and...

  • Streptococcus agalactiae in Brazil: serotype distribution, virulence determinants and antimicrobial susceptibility. Dutra, Vanusa G.; Alves, Valéria M. N.; Olendzki, André N.; Dias, Cicero A. G.; de Bastos, Alessandra F. A.; Santos, Gianni O.; de Amorin, Efigênia L. T.; Sousa, Meireille Â. B.; Santos, Rosemary; Ribeiro, Patricia C. S.; Fontes, Cleuber F.; Andrey, Marco; Magalhães, Kedma; Araujo, Ana A.; Paffadore, Lilian F.; Marconi, Camila; Murta, Eddie F. C.; Fernandes, Paulo C.; Raddi, Maria S. G.; Marinho, Penélope S. // BMC Infectious Diseases;2014, Vol. 14 Issue 1, p1 

    Background Group B Streptococcus (GBS) remains a major cause of neonatal sepsis and is also associated with invasive and noninvasive infections in pregnant women and non-pregnant adults, elderly and patients with underlying medical conditions. Ten capsular serotypes have been recognized, and...

  • Rate of Transmission of Extended-Spectrum Beta-Lactamase–Producing Enterobacteriaceae Without Contact Isolation. Tschudin-Sutter, Sarah; Frei, Reno; Dangel, Marc; Stranden, Anne; Widmer, Andreas F. // Clinical Infectious Diseases;Dec2012, Vol. 55 Issue 11, p1505 

    The estimated rate of spread of extended-spectrum beta-lactamaseproducing Enterobacteriaceae was low in a tertiary care university-affiliated hospital with high levels of standard hygiene precautions, challenging the routine use of contact isolation in a non-epidemic setting.Background....

  • Genotypic characterization of Staphylococcus aureus isolated from bovines, humans, and food in Indonesia. Salasia, Siti Isrina Oktavia; Tato, Syarifudin; Sugiyono, Ngalijan; Ariyanti, Dwi; Prabawati, Feny // Journal of Veterinary Science;Dec2011, Vol. 12 Issue 4, p353 

    The present study determined the genetic relationships between 41 Staphyloccocus (S.) aureus isolates from bovines, humans, and food using a single enzyme amplified fragment length polymorphism (AFLP) technique. We evaluated the prevalence of staphylococcal enterotoxin (SE) genes and other...

  • Genetic variability and virulence of Meloidogyne incognita populations from Brazil to resistant cotton genotypes. Silva, Esdras; Silva Mattos, Vanessa; Furlaneto, Cleber; Giband, Marc; Barroso, Paulo; Moita, Antônio; Jorge-Junior, Aldemiro; Correa, Valdir; Castagnone-Sereno, Philippe; Carneiro, Regina // European Journal of Plant Pathology;May2014, Vol. 139 Issue 1, p195 

    The root-knot nematode Meloidogyne incognita is widely distributed and a major pathogen of cotton ( Gossypium spp.) worldwide. The objectives of this study were to assess the genetic variability and aggressiveness of Brazilian populations of M. incognita in cotton. Five populations of M....

  • Prevalence of Escherichia coli carrying extended-spectrum β-lactamases (CTX-M and TEM-52) from broiler chickens and turkeys in Great Britain between 2006 and 2009. Randall, L. P.; Clouting, C.; Horton, R. A.; Coldham, N. G.; Wu, G.; Clifton-Hadley, F. A.; Davies, R. H.; Teale, C. J. // Journal of Antimicrobial Chemotherapy (JAC);Jan2011, Vol. 66 Issue 1, p86 

    Objectives To determine the prevalence of extended-spectrum β-lactamases (ESBLs) in Escherichia coli from poultry in Great Britain (GB). Methods E. coli was isolated from 388 broiler chicken caecal samples from 22 abattoirs and from boot swabs from 442 turkey flocks over successive 1 year...

  • Emergence and persistence of integron structures harbouring VIM genes in the Childrens Memorial Health Institute, Warsaw, Poland, 1998-2006. Jan A. Patzer; Timothy R. Walsh; Janis Weeks; Danuta Dzierzanowska; Mark A. Toleman // Journal of Antimicrobial Chemotherapy (JAC);Feb2009, Vol. 63 Issue 2, p269 

    : Objectives The aim was to perform a genetically detailed study of the emergence of metallo-β-lactamase (MBL) genes in Pseudomonas spp. in the Childrens Memorial Health Institute over a 9 year period. : Methods Carbapenem-resistant Pseudomonas spp. isolates were collected from 1998 to 2006...

  • UK epidemic Escherichia coli strains A-E, with CTX-M-15 {beta}-lactamase, all belong to the international O25:H4-ST131 clone. Siu Ha Lau; Mary E. Kaufmann; David M. Livermore; Neil Woodford; Geraldine A. Willshaw; Tom Cheasty; Katie Stamper; Suganya Reddy; John Cheesbrough; Frederick J. Bolton; Andrew J. Fox; Mathew Upton // Journal of Antimicrobial Chemotherapy (JAC);Dec2008, Vol. 62 Issue 6, p1241 

    : Objectives Uropathogenic and invasive Escherichia coli O25:H4-ST131 isolates producing CTX-M-15 extended-spectrum β-lactamase (ESBL) enzymes have recently been shown to be disseminated across the globe. In the UK, many CTX-M-15 ESBL-producing E. coli strains have been previously defined as...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics