Artifacts in Oral Biopsies: A Study

Shah, Arpan; Kulkarni, Meena; Gabhane, Mahesh
December 2012
Indian Journal of Stomatology;2012, Vol. 3 Issue 4, p217
Academic Journal
Aim: Artifacts are the integral part of each microscopic field. Sometimes they can create serious errors and misdiagnosis. Artifacts may arise as a result of faulty surgical technique or correspond to sample processing in the laboratory. Biopsies of the oral cavity are of small size and fine texture; as a result, artifacts are easily produced during processing, microtomy and staining procedures. It is important to identify the common occurring artifacts during interpretation of oral biopsies. Material and methods: A total of 50 oral biopsies (Hematoxylin and Eosin stained slides) were retrieved from the archives of the Department of Oral Pathology and Microbiology, M.G.V Dental College and Hospital, Nashik and were analyzed for the presence of various histopathological artifacts. The numbers of artifacts were determined both as an absolute number and as a percentage of the total number. Results: Different artifacts were observed, including changes produced by laser and electrocautery. Highest frequency (82%) of split artifacts (vertical/horizontal) and minimum frequency of hemorrhage and impurities (4%) were noted. Conclusion: Present study showed that a large number of artifacts can occur during processing, microtomy and staining. The most commonly occurring histopathological artifacts in biopsies of oral mucosal lesions are horizontal/vertical splits.


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