TITLE

Simultaneous spectrophotometric determination of cefixime and ofloxacin in pharmaceuticals by chemometric methods

AUTHOR(S)
Swamiappan, Yamuna; Arcot, Shantha
PUB. DATE
July 2012
SOURCE
Journal of Pharmacy Research;2012, Vol. 5 Issue 7, p3919
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In the present study, two different chemometric methods - partial least squares (PLS) and principal component regression (PCR) for simultaneous determination of cefixime and ofloxacin in pharmaceuticals are described. These two approaches were successfully applied to resolve the overlapped data and quantify the two components in the studied mixture using the calibrations constructed with the absorption data matrix corresponding to the concentration data matrix with measurements in the range of 210-380 nm (▵ λ = 1 nm) of the zero order spectra. The calibration range was found to be 2-10 μg mL-1 for both the drugs, cefixime and ofloxacin. The PLS and PCR methods neither require any separation step, nor any prior graphical treatment of the overlapping spectra of the two drugs in a mixture. The calibration of the chemometric models were evaluated by internal validation (prediction of components in its own designed training set of calibration) and by external validation over synthetic and pharmaceutical preparations. Both studied methods can be considered acceptable for the pharmaceutical quality assurance of cefixime and ofloxacin in combined dosage forms.
ACCESSION #
84425210

 

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