TITLE

Differential effects of nerve growth factor on expression of dopamine 2 receptor subtypes in GH3 rat pituitary tumor cells

AUTHOR(S)
Su, Zhipeng; Jiang, Xiaolong; Wang, Chengde; Liu, Jie; Chen, Yunxiang; Li, Qun; Wu, Jinsen; Zheng, Weiming; Zhuge, Qichuan; Jin, Kunlin; Wu, Zhebao
PUB. DATE
December 2012
SOURCE
Endocrine (1355008X);Dec2012, Vol. 42 Issue 3, p670
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Nerve growth factor (NGF) can increase expression of dopamine 2 receptors (D2R) in GH3 rat pituitary tumor cells (GH3 cells). As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtypes in GH3 cells treated with or without NGF with real-time RT-PCR. In addition, we also evaluated the relationship between GH3 cell growth after bromocriptine treatment and mRNA levels of D2R subtypes. We found that D2R total, D2L, and D2S mRNA in GH3 cells were significantly increased after NGF treatment, compared with the vehicle group. Moreover, NGF increased the ratio of D2S to D2L. GH3 cell survival rate after bromocriptine treatment was negatively correlated with D2R total mRNA, and D2S may be more potent than D2L in inhibiting cell growth. Cell apoptosis rate was highly elevated in GH3 cells treated with NGF and bromocriptine, compared with the control group or the group treated with NGF or bromocriptine alone. Our data provide preliminary evidence that the effect of NGF was more prominent on expression of D2S than D2L, in addition, D2S might have a greater impact suppressing GH3 cells growth than D2L.
ACCESSION #
83709706

 

Related Articles

  • Nerve growth factor selectively regulates expression of transcripts encoding ribosomal proteins. Angelastro, James M.; Töröcsik, Béata; Greene, Lloyd A.; Töröcsik, Béata // BMC Neuroscience;2002, Vol. 3, p3 

    Background: NGF exerts a variety of actions including promotion of neuronal differentiation and survival. The PC12 rat pheochromocytoma cell line has proved valuable for studying how NGF works and has revealed that the NGF mechanism includes regulation of gene expression....

  • Neurotrophins and Neurotrophin Receptors in Proliferative Diabetic Retinopathy. Abu El-Asrar, Ahmed M.; Mohammad, Ghulam; De Hertogh, Gert; Nawaz, Mohd Imtiaz; Van Den Eynde, Kathleen; Siddiquei, Mohammad Mairaj; Struyf, Sofie; Opdenakker, Ghislain; Geboes, Karel // PLoS ONE;Jun2013, Vol. 8 Issue 6, p1 

    Neurotrophins (NTs) are emerging as important mediators of angiogenesis and fibrosis. We investigated the expression of the NTs nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) and their receptors TrkA, TrkB, and TrkC in...

  • Sortilin Expression Is Essential for Pro-Nerve Growth Factor-Induced Apoptosis of Rat Vascular Smooth Muscle Cells. Campagnolo, Luisa; Costanza, Gaetana; Francesconi, Arianna; Arcuri, Gaetano; Moscatelli, Ilana; Orlandi, Augusto // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Background: Sortilin, a member of the Vps10p-domain receptor family, has been demonstrated a key regulator in mediating cellular response to pro-neurotrophins. In the present study, we investigated the role of sortilin in the apoptotic pathway of vascular smooth muscle cells. Methods and...

  • Dual control of neurite outgrowth by STAT3 and MAP kinase in PC12 cells stimulated with interleukin-6. Ihara, Sayoko; Nakajima, Koichi; Fukada, Toshiyuki; Hibi, Masahiko; Nagata, Satoshi; Hirano, Toshio; Fukui, Yasuhisa // EMBO Journal;9/1/97, Vol. 16 Issue 17, p5345 

    IL-6 induces differentiation of PC12 cells pretreated with nerve growth factor (NGF). We explored the signals required for neurite outgrowth of PC12 cells by using a series of mutants of a chimeric receptor consisting of the extracellular domain of the granulocyte-colony stimulating factor...

  • LAMTOR2-Mediated Modulation of NGF/MAPK Activation Kinetics during Differentiation of PC12 Cells. Thauerer, Bettina; Voegele, Paul; Hermann-Kleiter, Natascha; Thuille, Nikolaus; de Araujo, Mariana E. G.; Offterdinger, Martin; Baier, Gottfried; Huber, Lukas A.; Baier-Bitterlich, Gabriele // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    LAMTOR2 (p14), a part of the larger LAMTOR/Ragulator complex, plays a crucial role in EGF-dependent activation of p42/44 mitogen-activated protein kinases (MAPK, ERK1/2). In this study, we investigated the role of LAMTOR2 in nerve growth factor (NGF)-mediated neuronal differentiation....

  • Selective increase of dopamine D3 receptor gene expression as a common effect of chronic antidepressant treatments. Lammers, C.-H.; Diaz, J.; Schwartz, J.-C.; Sokoloff, P. // Molecular Psychiatry;2000, Vol. 5 Issue 4, p378 

    The mesolimbic dopaminergic system is a neuroanatomical key structure for reward and motivation upon which previous studies indicated that antidepressant drugs exert a stimulatory influence, via still unknown neurobiological mechanisms. Here we examined the effects of chronic administration of...

  • Specific growth suppression of human cancer cells by targeted delivery of Dictyostelium mitochondrial ribosomal protein S4. Junji Chida; Hikaru Araki; Yasuo Maeda // Cancer Cell International;2014, Vol. 14 Issue 1, p1 

    Background In general, growth and differentiation are mutually exclusive but are cooperatively regulated throughout development. Thus, the process of a cell's switching from growth to differentiation is of great importance not only for the development of organisms but also for malignant...

  • Expression of the Oxidative Stress-Regulated Transcription Factor Bach2 in Differentiating Neuronal Cells1. Hoshino, Hideto; Igarashi, Kazuhiko // Journal of Biochemistry;2002, Vol. 132 Issue 3, p427 

    Bach2 is an oxidative stress-regulated transcription factor and functions as a repressor of gene expression directed by the TPA-response element, the Maf recognition element, and the antioxidant responsive element. To investigate the possibility that these enhancers are regulated in a...

  • Bioinformatics analysis to identify the differentially expressed genes of glaucoma. XIANG YAN; FEI YUAN; XIUPING CHEN; CHUNQIONG DONG // Molecular Medicine Reports;2015, Vol. 12 Issue 4, p4829 

    The aim of the present study was to screen the differentially expressed genes (DEGs) associated with glaucoma and investigate the changing patterns of the expression of these genes. The GSE2378 gene microarray data of glaucoma was downloaded from the Gene Expression Omnibus database, which...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics