Analysis of p53, K-ras gene mutation & Helicobacter pylori infection in patients with gastric cancer & peptic ulcer disease at a tertiary care hospital in north India

Saxena, Ashish; Shukla, Sanket Kumar; Prasad, Kashi Nath; Ghoshal, Uday Chand
October 2012
Indian Journal of Medical Research;Oct2012, Vol. 136 Issue 4, p664
Academic Journal
Background & objectives: Mutations in the oncogene and tumour suppressor genes play an important role in carcinogenesis. We investigated the association of p53 and K-ras gene mutation and Helicobacter pylori infection in patients with gastric cancer (GC) and peptic ulcer disease (PUD) attending a tertiary care hospital in north India. Methods: In total, 348 adult patients [62 GC, 45 PUD and 241 non-ulcer dyspepsia (NUD)] who underwent an upper gastrointestinal endoscopy were enrolled. H. pylori infection was diagnosed by rapid urease test, culture, histopathology and PCR. Mutation in the exon 5-8 of p53 gene was analyzed by PCR-single stranded conformational polymorphism (SSCP) and confirmed by sequence analysis. K-ras gene codon 12 mutation was analyzed by PCR-based restriction fragment length polymorphism. Results: Overall p53 gene mutation was found in 4.6 per cent of the study population, and its distribution in GC, PUD and NUD was 21, 4.4 and 0.4 per cent, respectively. p53 gene mutation was significantly higher in patients with GC than PUD (P<0.05) and NUD (P<0.001). No difference in p53 gene mutation was observed between H. pylori infected and non-infected individuals. K-ras gene mutation was absent in all the patients. Interpretation & conclusions: Our results show that p53 gene mutation may be associated with gastric carcinogenesis independent to H. pylori infection and absence of K-ras gene mutation questions its role in the pathogenesis of GC and PUD in Indian patients.


Related Articles

  • Good news for gastric sufferers. Hunter, Beatrice Trum // Consumers' Research Magazine;Oct93, Vol. 76 Issue 10, p8 

    Focuses on the possible role of Helicobacter pylori in peptic ulcers. Response to antibiotic treatment; Discovery of H. pylori; Unique characteristics of H. pylori; Number of new cases of stomach cancer annually in the United States; More.

  • Helicobacter pylori-positive patients with pruritic skin diseases are a high-risk group of gastric cancer patients: a population based study. Zagari, R.; Casanova, S.; Fiocca, R.; Pozzato, P.; Martuzzi, C.; Ricciardiello, L.; De Luca, L.; Maltoni, S.; Fuccio, L.; Lunedei, V.; Di Marco, M.; D'Errico, A.; Grigioni, W.; Panuccio, D.; Cioffi, G.; Roda, E.; Bazzoli, F. // Gut;Sep2002 Supplement 2, Vol. 51, pA54 

    Background: H. pylori infection is associated with peptic ulcer disease and gastric cancer and has also been implicated as a possible cause of extraintestinal disorders such as skin diseases. There are a few case reports on patients with skin diseases, such as prurigo nodularis that are...

  • The [sup 13]C-urea breath (UBT) level in gastric cancer in comparison with peptic ulcer and gastritis. Wu, J.Y.; Tseng, C.A.; Yu, F.J.; Kuo, C.H.; Lu, C.Y.; Su, Y.C.; Wu, D.C.; Perng, D.S.; Chen, L.T.; Jan, C.M.; Chen, C.Y.; Wang, W.M. // Gut;Sep2002 Supplement 2, Vol. 51, pA57 

    Purpose: The UBT is considered to be a reliable, non-invasive method for diagnosis of H.p infection. Results are related to the severity of atrophic gastritis and density of H.p population which is often associated with gastric cancer. This study is to determine the result of UBT in gastric...

  • Efficacy of different therapies for Helicobacter pylori eradication: A 5-year experience in a single center. Liao, C.C.; Lee, C.L.; Lee, H.Y.; Wu, C.H.; Chen, T.K.; Tu, T.C. // Gut;Sep2002 Supplement 2, Vol. 51, pA97 

    Background: H. pylori is an important etiologic factor for peptic ulcer disease, gastric cancer and MALT lymphoma. There is a general agreement that H. pylori should be eradicated in these patients. However, eradication rates are different according to the anti-secretory drugs and antibiotics...

  • Diagnosis of Helicobacter pylori in Britain. Duggan, Anne; Logan, Robert // Journal of Gastroenterology;1998 Supplement 10, Vol. 33, p31 

    The prevalence of Helicobacter pylori infection in the United Kingdom (UK) is similar to that of other developed Western countries with H. pylori infection a major cause of morbidity from peptic ulcer disease and gastric cancer. Guidelines for testing and treating H. pylori have been published...

  • The Impact of Peptic Ulcer Disease and Infection With Helicobacter pylori on Life Expectancy. Inadomi, John M.; Sonnenberg, Amnon // American Journal of Gastroenterology (Springer Nature);Aug1998, Vol. 93 Issue 8, p1286 

    Objective: Knowledge about the influence of H. pylori-related disease on life expectancy might affect physician behavior in dealing with such disease. The aim of this study was to assess how life expectancy is influenced by H. pylori infection and peptic ulcer disease. Methods:...

  • Helicobacter pylori-negative gastric and duodenal ulcers. Tsuji, Hideharu; Kohli, Yoshihiro; Fukumitsu, Shinji; Morita, Kiyoshi; Kaneko, Hiroto; Ohkawara, Tohru; Minami, Masahito; Ueda, Kazushige; Sawa, Yoshihiko; Matsuzaki, Haruki; Morinaga, Osamu; Ohkawara, Yasuo; Tsuji, H; Kohli, Y; Fukumitsu, S; Morita, K; Kaneko, H; Ohkawara, T; Minami, M; Ueda, K // Journal of Gastroenterology;1999, Vol. 34 Issue 4, p455 

    It is unclear whether Helicobacter pylori infection is essential to the development of peptic ulcers. In this study, we examined the rates of H. pylori-negativity among patients with peptic ulcers. We also attempted to clarify the characteristics of H. pylori-negative peptic ulcers to throw...

  • Childhood ulcers.  // Pediatrics for Parents;1995, Vol. 16 Issue 4, p1 

    Reports on the incidence of peptic ulcers in children. Infection with Helicobacter pylori.

  • Ulcers and bacteria--the role of H. pylori.  // American Family Physician;11/1/1995, Vol. 52 Issue 6, p1729 

    Cites the role of Helicobacter pylori in the development of peptic ulcers. Importance of treating H. pylori infection; All patients with stomach and duodenal ulcers as needing HH. pylori infection treatment; Antibiotic treatment for H. pylori infection.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics