La gD2 coadministrada con el AFCo1 por vía intranasal induce inmunidad protectora contra virus de herpes simple tipo 2 en ratones

Cabrera, Osmir; Cuello, Maribel; Thörn, Karolina; Peerson, Josefine; Lindqvist, Madelene; Lastre, Miriam; González, Elizabeth; Zayas, Caridad; Balboa, Julio; Romeo, Belkis; Sarandi, Ali; Pérez, Oliver
September 2012
Revista VacciMonitor (Vacunología y Temas Afines);sep-dic2012, Vol. 21 Issue 3, p19
Academic Journal
Sexually transmitted infections by Herpes Simplex Virus type 2 (HSV-2) are the leading cause of genital ulcers and a major public health problem worldwide. This requires the use of mucosal vaccines, because parenteral vaccines have not been successful. Presently, there are not mucosal adjuvants, for this reason the development of adjuvants is essential for mucosal vaccine strategies. The intranasal (IN) immunizations using HSV-2 glycoprotein D (gD2), coadministered with cochleate (AFCo1+gD2), would be an efficient candidate for future vaccines against HSV2, similar to the gD2 incorporated into AFCo1(AFCo1-gD2). Female C57Bl/6 mice were inoculated with AFCo1-gD2, AFCo1+gD2 or gD2 alone by IN route. The anti gD2 IgG in sera and vaginal fluids and IgG subclasses were measured by ELISA. The lymphoproliferative response in spleen cells, the Th1/Th2 cytokine profile, the protection and the signs of disease against viral challenge were measured. High titers of IgG and IgG2c subclasses were observed in sera of mice that received the gD2 and AFCo1 as adjuvant. No significant differences (p>0.005) were observed in the animals that received AFCo1+gD2 or AFCo1-gD2. a preferential Th1 cytokine profile and 100% of survival after challenge were observed in both groups that received the gD2 and AFCo1, while no survival was observed in the group that only received the gD2. These results showed that the gD2 can be used coadministered with AFCo1 by IN route as a potential vaccine candidate against HSV-2.


Related Articles

  • Delayed but effective induction of mucosal memory immune responses against genital HSV-2 in the absence of secondary lymphoid organs. Roth, K L; Bhavanam, S; Jiang, H; Gillgrass, A; Ho, K; Ferreira, V H; Kaushic, C // Mucosal Immunology (1933-0219);Jan2013, Vol. 6 Issue 1, p56 

    To examine whether local immunization in the absence of secondary lymphoid organs (SLOs) could establish effective antiviral memory responses in the female genital tract, we examined immunity in the vaginal tracts of LTα−/− mice, LTα−/− SPL (splenectomized), and...

  • Pan-HSV-2 IgG Antibody in Vaccinated Mice and Guinea Pigs Correlates with Protection against Herpes Simplex Virus 2. Halford, William P.; Geltz, Joshua; Gershburg, Edward // PLoS ONE;Jun2013, Vol. 8 Issue 6, p1 

    We lack a correlate of immunity to herpes simplex virus 2 (HSV-2) that may be used to differentiate whether a HSV-2 vaccine elicits robust or anemic protection against genital herpes. This gap in knowledge is often attributed to a failure to measure the correct component of the adaptive immune...

  • Clinical and Therapeutic Issues for Herpes Simplex Virus-2 and HIV Co-Infection. Lingappa, Jairam R.; Celum, Connie // Drugs;2007, Vol. 67 Issue 2, p155 

    A synergy between HIV type-1 (HIV-1) and herpes simplex virus-2 (HSV-2) has been demonstrated in many epidemiological and clinical studies over the last decade. HIV-1 infection exacerbates the clinical impact and frequency of HSV-2 reactivation events; furthermore, HSV-2 infection exacerbates...

  • MUCOSAL IMMUNIZATION FOR IMMUNITY TO PRIMARY AND RECURRENT GENITAL HERPES. Persson, J.; Thorn, K.; Harandi, A.M. // Revista VacciMonitor (Vacunología y Temas Afines);2010 Supplement 1, Vol. 19, p19 

    Herpes simplex virus type 2 (HSV-2) is a major cause of genital herpes, and one of the most common sexually transmitted infections. Following infection, many individuals experience life-long latency associated with sporadic ulcerative outbreaks. Despite many efforts no vaccine has yet been...

  • Prevention of Genital Herpes Simplex Virus Type 1 and 2 Disease in Mice Immunized with a gD-Expressing Dominant-Negative Recombinant HSV-1. Brans, Richard; Akhrameyeva, Natali V.; Feng Yao // Journal of Investigative Dermatology;Oct2009, Vol. 129 Issue 10, p2470 

    CJ9-gD is a novel herpes simplex virus (HSV) type 1 recombinant virus that is completely replication-defective, expresses high-levels of HSV-1 major antigen glycoprotein D (gD), and can function in trans to inhibit replication of wild-type HSV-1 and HSV-2 in co-infected cells. Here, we show that...

  • Serological Profile of HSV-2 in STD Patients: Evaluation of Diagnostic Utility of HSV-2 IgM and IgG Detection. AMUDHA, V. P.; RASHETHA; SUCILATHANGAM, G.; CINTHUJAH, B.; REVATHY, C. // Journal of Clinical & Diagnostic Research;Dec2014, Vol. 8 Issue 12, p16 

    Background and Objectives: The present study was undertaken to determine Herpes Simplex Virus-2 seroprevalence in sexually active adults aged 20-49 and to investigate the correlation with sociodemographic characteristics and to find its association with other sexually transmitted diseases...

  • Seroprevalence, predictors and estimated incidence of maternal and neonatal Herpes Simplex Virus Type 2 infection in semi-urban women in Kilifi, Kenya. Nyiro, Joyce U.; Sanders, Eduard J.; Ngetsa, Caroline; Wale, Steve; Awuondo, Ken; Bukusi, Elizabeth; Price, Matthew A.; Amornkul, Pauli N.; Nokes, D James // BMC Infectious Diseases;2011, Vol. 11 Issue 1, p155 

    Background: Herpes Simplex Virus type 2 (HSV-2) has public health importance as a leading cause of genital ulcers, a co-factor in HIV-1 acquisition and transmission and as a cause of neonatal herpes infections. Little is known of its epidemiology and burden in Coastal Kenya. Methods: We screened...

  • IgG in cervicovaginal mucus traps HSV and prevents vaginal Herpes infections. Wang, Y-Y; Kannan, A; Nunn, K L; Murphy, M A; Subramani, D B; Moench, T; Cone, R; Lai, S K // Mucosal Immunology (1933-0219);Sep2014, Vol. 7 Issue 5, p1036 

    IgG is the predominant immunoglobulin in cervicovaginal mucus (CVM), yet how immunoglobulin G (IgG) in mucus can protect against infections is not fully understood. IgG diffuses rapidly through cervical mucus, slowed only slightly by transient adhesive interactions with mucins. We hypothesize...

  • The Relationship between Condom Use and Herpes Simplex Virus Acquisition. Wald, Anna; Langenberg, Andria G. M.; Krantz, Elizabeth; Douglas, Jr., John M.; Handsfield, H. Hunter; DiCarlo, Richard P.; Adimora, Adaora A.; Izu, Allen E.; Morrow, Rhoda Ashley; Corey, Lawrence // Annals of Internal Medicine;11/15/2005, Vol. 143 Issue 10, p707 

    Background: Few studies have evaluated the relationship between condom use and herpes simplex virus type 2 (HSV-2) and HSV type 1 (HSV-1) acquisition. Objective: To assess the relationship between condom use and acquisition of HSV-2 and HSV-1 among men and women. Design: Analysis of data...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics