TITLE

Expression of Maize Gene Encoding C-Pyruvate Orthophosphate Dikinase ( PPDK) and C-Phosphoenolpyruvate Carboxylase ( PEPC) in Transgenic Arabidopsis

AUTHOR(S)
Wang, Yu-Min; Xu, Wei-Gang; Hu, Lin; Zhang, Lei; Li, Yan; Du, Xi-He
PUB. DATE
December 2012
SOURCE
Plant Molecular Biology Reporter;Dec2012, Vol. 30 Issue 6, p1367
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
To improve the efficiency of CO fixation in C photosynthesis, C-cycle genes were overexpressed in Arabidopsis plants. In this study, a full-length cDNA for pyruvate orthophosphate dikinase ( PPDK) with 971 amino acids was isolated from Zea mays using LA-PCR. The PPDK gene (GenBank accession number GU363532) and the phosphoenolpyruvate carboxylase ( PEPC) gene (GenBank accession number: FJ415327) derived from a previous study were introduced into wild-type Arabidopsis both individually and in combination. The vector pCAMBIA3301 (p3301) was introduced into Arabidopsis as a control using an Agrobacterium-mediated system. The integration, transcription, and translation of the PPDK and the PEPC genes were confirmed by Southern blot, quantitative real-time PCR, and Western blot analysis. The average enzyme activity of C- PPDK and C- PEPC peaked at 4.63- and 5.81-fold compared to that of controls, respectively. The insertion of PPDK was found to increase the activity of PEPCase and vice versa. The photosynthetic rates of C- PPDK, C- PEPC, and C- PKC( PPDK+ PEPC) transgenic lines increased to 123.3, 136.4, and 146.6 % compared to that of controls, respectively.
ACCESSION #
82732185

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics