TITLE

Lipsome-mediated uptake of exogenous DNA by Sahiwal cattle spermatozoa

AUTHOR(S)
Babu, Thomas V.; Pratheesh, M. D.; Anandlaxmi, N.; Amarnath, K.
PUB. DATE
October 2012
SOURCE
Veterinary World;Oct2012, Vol. 5 Issue 10, p621
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aim: To investigate the influence of lipofection treatment and exogenous DNA uptake on the quality of sahiwal cattle spermatozoa. Materials and Methods: Semen collected from sahiwal bulls (n=7) were evaluated separately for color, volume, mass activity, concentration, motility and viability using standard procedures. Pooled sperm samples from selected bulls (n=3) were transfected with a model gene construct enhanced green fluorescent protein (p-EGFP) via lipofection method and confirmed the genome integration by PCR technique. Furthermore the effect of transfection on spermatozoa was assessed based on apotosis, viability and motility. Results: In the current investigation sahiwal bulls were selected based on their breeding records and better semen characteristics. Although the transfected sperm samples failed to show florescence under fluorescence microscope, PCR studies confirmed the successful uptake of the p-EGFP gene in to the host sperm cell genome. Moreover transfected samples showed a significant reduction in the viability and motility without causing any DNA damage induced apoptosis as demonstrated by DNALadder assay.
ACCESSION #
82716384

 

Related Articles

  • Visualisation of the mechanosensitive channel of large conductance in bacteria using confocal microscopy. Norman, Christel; Zhen-Wei-Liu; Rigby, Paul; Raso, Albert; Petrov, Yevgeniy; Martinac, Boris // European Biophysics Journal;Jul2005, Vol. 34 Issue 5, p396 

    The mechanosensitive channel of large conductance (MscL) plays an important role in the survival of bacterial cells to hypo-osmotic shock. This channel has been extensively studied and its sequence, structure and electrophysiological characteristics are well known. Here we present a method to...

  • Development and Evaluation of Transgenic Nude Mice Expressing Ubiquitous Green Fluorescent Protein. Iyer, Srikanth; Arindkar, Shailendra; Mishra, Alaknanda; Manglani, Kapil; Kumar, Jerald; Majumdar, Subeer; Upadhyay, Pramod; Nagarajan, Perumal // Molecular Imaging & Biology;Aug2015, Vol. 17 Issue 4, p471 

    Purpose: Researchers had developed and characterized transgenic green/red fluorescent protein (GFP/RFP) nude mouse with ubiquitous RFP or GFP expression, but none has evaluated the level of immune cells and expression levels of GFP in this model. Procedure: The nude GFP mice were evaluated by...

  • GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS. Singh, Sanjeev; Kankoriya, Sarita; Kumar Jain, Sudhir; Jain, Hemlata; Pal Singh, Satyendra // Indian Journal of Animal Research;Oct2014, Vol. 48 Issue 5, p418 

    15 microsatellite loci were investigated for studying the genetic polymorphism in Sahiwal (30) and Tharparker (30) breeds of cattle. Polymerase chain reactions (PCR) were carried out and amplified products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 15...

  • Incidence of Staphylococci and Streptococci During Winter in Mastitic Milk of Sahiwal Cow and Murrah Buffaloes. Sentitula; Yadav, B.; Kumar, Ravinder // Indian Journal of Microbiology;Jun2012, Vol. 52 Issue 2, p153 

    Mastitis is a serious problem in dairy sector and among various aetiological agents, the incidence of staphylococci and streptococci remains high in milking animal. The present study was focused on detection of staphylococci and streptococci in winter season. Milk samples (117) of mastitic...

  • A multi-needle-assisted transformation of soybean cotyledonary node cells. Xue, Ren-Gao; Xie, Hong-Feng; Zhang, Biao // Biotechnology Letters;Oct2006, Vol. 28 Issue 19, p1551 

    A new and simple method for wounding cotyledonary node cells of soybean [ Glycine max (L) Merrill] was developed for obtaining a high frequency of transformants. Soybean seeds were germinated for 1 day, and the cotyledonary node cells of half-seeds were wounded mechanically by using a...

  • A bioremediation approach using natural transformation in pure-culture and mixed-population biofilms. Perumbakkam, Sudeep; Hess, Thomas F.; Crawford, Ronald L. // Biodegradation;Dec2006, Vol. 17 Issue 6, p545 

    Bacterial transformation by naked DNA is thought to contribute to gene transfer and microbial evolution within natural environments. In nature many microbial communities exist as complex assemblages known as biofilms where genetic exchange is facilitated. It may be possible to take advantage of...

  • The cation-efflux transporter BjCET2 mediates zinc and cadmium accumulation in Brassica juncea L. leaves. Jin Xu; Tuanyao Chai; Yuxiu Zhang; Minglin Lang; Lu Han // Plant Cell Reports;Aug2009, Vol. 28 Issue 8, p1235 

    Abstract   Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of...

  • Isolation of GPD Promoter from Tremella fuciformis and Driving Expression of EGFP Gene. Shu-Jing Sun; Dong-Xing Chen; Bao-Gui Xie; Fang-Ping Hu; Jin-Gui Zheng // DNA & Cell Biology;Feb2009, Vol. 28 Issue 2, p65 

    Glyceraldehyde-3-phosphate dehydrogenase (GPD) cDNA was cloned by RT-PCR using total RNA from Tremella fuciformis as template with a pair of degenerate primers. Then, a 500-bp 5′-upstream promoter region of the gene encoding GPD from T. fuciformis genomic DNA was isolated by thermal...

  • Overexpressing a Putative Aquaporin Gene from Wheat, TaNIP, Enhances Salt Tolerance in Transgenic Arabidopsis. Zhenxian Gao; Xiaoliang He; Baocun Zhao; Chunjiang Zhou; Yingzhu Liang; Rongchao Ge; Yinzhu Shen; Zhanjing Huang // Plant & Cell Physiology;May2010, Vol. 51 Issue 5, p767 

    High soil salinity is a major abiotic stress in plant agriculture worldwide. Here, we report the characterization of a novel aquaporin gene TaNIP (Triticum asetivum L. nodulin 26-like intrinsic protein), which was involved in salt tolerance pathways in plants. TaNIP was identified and cloned...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics