VpWRKY3, a biotic and abiotic stress-related transcription factor from the Chinese wild Vitis pseudoreticulata

Zhu, Ziguo; Shi, Jiangli; Cao, Jiangling; He, Mingyang; Wang, Yuejin
November 2012
Plant Cell Reports;Nov2012, Vol. 31 Issue 11, p2109
Academic Journal
Chinese wild grapevine Vitis pseudoreticulata accession 'Baihe-35-1' is identified as the precious resource with multiple resistances to pathogens. A directional cDNA library was constructed from the young leaves inoculated with Erysiphe necator. A total of 3,500 clones were sequenced, yielding 1,727 unigenes. Among them, 762 unigenes were annotated and classified into three classes, respectively, using Gene Ontology, including 22 ESTs related to transcription regulator activity. A novel WRKY transcription factor was isolated from the library, and designated as VpWRKY3 (GenBank Accession No. JF500755). The full-length cDNA is 1,280 bp, encoding a WRKY protein of 320 amino acids. VpWRKY3 is localized to nucleus and functions as a transcriptional activator. QRT-PCR analysis showed that the VpWRKY3 specifically accumulated in response to pathogen, salicylic acid, ethylene and drought stress. Overexpression of VpWRKY3 in tobacco increased the resistance to Ralstonia solanacearum, indicating that VpWRKY3 participates in defense response. Furthermore, VpWRKY3 is also involved in abscisic acid signal pathway and salt stress. This experiment provided an important basis for understanding the defense mechanisms mediated by WRKY genes in China wild grapevine. Generation of the EST collection from the cDNA library provided valuable information for the grapevine breeding. Key message We constructed a cDNA library from Chinese wild grapevine leaves inoculated with powdery mildew. VpWRKY3 was isolated and demonstrated that it was involved in biotic and abiotic stress responses.


Related Articles

  • Role of Salicylic Acid and NIMI.Npri in Race-Specific Resistance in Arabidopsis. Rairdan, Gregory J.; Delaney, Terrence P. // Genetics;Jun2002, Vol. 161 Issue 2, p803 

    Examines the role of salicylic acid (SA) and the NIMI/NPR1 protein in race-specific Arabidopsis resistance. Recognition of pathogens from plants; Determination of requirements for both race-specific and systemic acquired resistance; Correlation of R-protein structure or pathogen specificity...

  • Resistant and Susceptible Responses in Tomato to Cyst Nematode are Differentially Regulated by Salicylic Acid. Uehara, Taketo; Sugiyama, Shunpei; Matsuura, Hideyuki; Arie, Tsutomu; Masuta, Chikara // Plant & Cell Physiology;Sep2010, Vol. 51 Issue 9, p1524 

    To understand the machinery underlying a tomato cultivar harboring the Hero A gene against cyst nematode using microarrays, we first analyzed tomato gene expression in response to potato cyst nematode (PCN; Globodera rostochiensis) during the early incompatible and compatible interactions at 3...

  • An important role of the pepper phenylalanine ammonia-lyase gene (PAL1) in salicylic acid-dependent signalling of the defence response to microbial pathogens. Kim, Dae Sung; Hwang, Byung Kook // Journal of Experimental Botany;Mar2014, Vol. 65, p1 

    The article discusses the role of the pepper phenylalanine ammonia-lyase gene (PAL1) in regulating defense response to microbial pathogens as of 2014. The topics discussed include salicylic acid -dependent singalling of the defense response, role of PAL in secondary phenylprpanoid metabolism....

  • SIGNIFICANCE OF ANTIOXIDANT ENZYMES IN STRESS SIGNALING. Naz, Sabiha; Pandey, Bhawana; Shrivastava, Awadhesh Kumar // Advances in Pharmacology & Toxicology;Aug2012, Vol. 13 Issue 2, p47 

    Plant expose to stress undergo changes in their metabolism in order to adapt to the changes in their environment. The present study was conducted to reveal the role of antioxidant enzymes like superoxide dismutase and peroxidase during acetyl salicylic acid and NaCl signaling in W. sominifera...

  • Preformed expression of defense is a hallmark of partial resistance to rice blast fungal pathogen Magnaporthe oryzae. Vergne, Emilie; Grand, Xavier; Ballini, Elsa; Chalvon, Véronique; Saindrenan, P.; Tharreau, D.; Nottéghem, J.-L.; Morel, J.-B. // BMC Plant Biology;2010, Vol. 10, p206 

    Background: Partial resistance to plant pathogens is extensively used in breeding programs since it could contribute to resistance durability. Partial resistance often builds up during plant development and confers quantitative and usually broad-spectrum resistance. However, very little is known...

  • Boosting plant immunity. Jamison, Judy // Nature Biotechnology;Jul2000, Vol. 18 Issue 7, p703 

    Studies the role of salicylic acid in inducing physiological responses, systemic acquired resistance in plants. Effect of salicylic acid on plant pathogens; Application of the technique on Escherichia coli and Pseudomonas fluorescens.

  • E-2-hexenal promotes susceptibility to Pseudomonas syringae pv. tomato by activating jasmonic acid pathways in Arabidopsis. Scala, Alessandra; Mirabella, Rossana; Mugo, Cynthia; Matsui, Kenji; Haring, Michel A.; Schuurink, Robert C. // Frontiers in Plant Science;Mar2013, Vol. 4, Special section p1 

    Green Leaf Volatiles (GLVs) are C6-molecules - alcohols, aldehydes and esters - produced by plants upon herbivory or during pathogen infection. Exposure to this blend of volatiles induces defence-related responses in neighboring undamaged plants, thus assigning a role to GLVs in regulating plant...

  • Transcriptome profiling of genes induced by salicylic acid and methyl jasmonate in Polygonum minus. Ee, Su-Fang; Oh, Ji-Min; Mohd Noor, Normah; Kwon, Taek-Ryoun; Mohamed-Hussein, Zeti-Azura; Ismail, Ismanizan; Zainal, Zamri // Molecular Biology Reports;Mar2013, Vol. 40 Issue 3, p2231 

    The importance of plant secondary metabolites for both mankind and the plant itself has long been established. However, despite extensive research on plant secondary metabolites, plant secondary metabolism and its regulation still remained poorly characterized. In this present study,...

  • Isolation and Functional Analysis of ZmLTP3, a Homologue to Arabidopsis LTP3. Hua-Wen Zou; Xiao-Hai Tian; Guo-Hui Ma; Zhi-Xin Li // International Journal of Molecular Sciences;Mar2013, Vol. 14 Issue 3, p5025 

    Plant lipid transfer proteins (LTPs) are encoded by multigene families and play important roles in plant physiology. One full-length cDNA encoding an Arabidopsis LTP3 homologue was isolated from maize by RT-PCR and named as ZmLTP3. RT-PCR analysis indicated that the ZmLTP3 expression is induced...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics