Novel role for a serine/arginine-rich splicing factor, AdRSZ21 in plant defense and HR-like cell death

Kumar, Koppolu; Kirti, P.
November 2012
Plant Molecular Biology;Nov2012, Vol. 80 Issue 4/5, p461
Academic Journal
A splicing factor gene belonging to the serine/arginine (SR)-rich protein family was cloned from Arachis diogoi, a wild relative of peanut in a study on differential gene expression and was designated as AdRSZ21. AdRSZ21 exhibits a RNA recognition motif (RRM), a CCHC type zinc finger domain (Zinc Knuckle, ZnK) and a C-terminal RS domain that is rich in arginine and serine. Multiple sequence alignment of AdRSZ21 with putative orthologs from diverse taxa including lower plants and monocots showed that the RRM and ZnK domains are evolutionarily conserved. Phylogenetic studies revealed that AdRSZ21 belongs to the RSZ subfamily and is closely related to the Arabidopsis ortholog AtRSZ22. Transient constitutive and conditional heterologous expression of AdRSZ21 resulted in HR-like cell death in tobacco leaves. The presence of a functional RRM domain, but not ZnK domain was essential for AdRSZ21 induced HR-like cell death phenotype. On the other hand, expression of AdRSZ21 with mutated ZnK domain lead to accelerated cell death. The cell death induced by AdRSZ21 was found to be associated with specific upregulation of patatin-like protein gene and other defense related gene transcripts suggesting a role for AdRSZ21 in plant defense and HR-like cell death.


Related Articles

  • A specific SR protein binds preferentially to the secretory protein gene transcripts in salivary glands of Chironomus tentans. Singh, Om Prakash; Visa, Neus; Wieslander, Lars; Daneholt, Bertil // Chromosoma;Dec2006, Vol. 115 Issue 6, p449 

    The members of the serine–arginine (SR) family of proteins play multiple roles in posttranscriptional gene expression. Initially considered as essential splicing factors confined to the nucleus and regulating constitutive and alternative splicing, SR proteins are now known to shuttle...

  • Molecular interplay between T-Antigen and splicing factor, arginine/serine-rich 1 (SRSF1) controls JC virus gene expression in glial cells. Craigie, Michael; Regan, Patrick; Otalora, Yolanda-Lopez; Kudret Sariyer, Ilker // Virology Journal;11/24/2015, Vol. 12 Issue 1, p1 

    Background: Human polyomavirus JCV is the etiologic agent of progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease characterized by lytic infection of glial cells in the central nervous system. PML is seen primarily in immunosuppressed patients and is mainly classified...

  • SR proteins and galectins: what's in a name? Haudek, Kevin C.; Patterson, Ronald J.; Wang, John L. // Glycobiology;Oct2010, Vol. 20 Issue 10, p1199 

    Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydratebinding activity at the cell surface. In contrast, galectins...

  • De-regulation of common housekeeping genes in hepatocellular carcinoma. Waxman, Samuel; Wurmbach, Elisa // BMC Genomics;2007, Vol. 8, p243 

    Background: Tumorigenesis is associated with changes in gene expression and involves many pathways. Dysregulated genes include "housekeeping" genes that are often used for normalization for quantitative real-time RT-PCR (qPCR), which may lead to unreliable results. This study assessed eight...

  • Identification of a family of DNA-binding proteins with homology to RNA splicing factors. Shipman, Kristy L.; Robinson, Phillip J.; King, Bruce R.; Smith, Roger; Nicholson, Richard C. // Biochemistry & Cell Biology;Feb2006, Vol. 84 Issue 1, p9 

    We describe a unique family of human proteins that are capable of binding to the cAMP regulatory element (CRE) and that are homologous to RNA splicing proteins. A human cDNA was isolated that encodes a protein with a distinctive combination of modular domain structures: 2 leucine-zipper-like...

  • Analysis of the functional specificity of RS domains in vivo. Dauwalder, Brigitte; Mattox, William // EMBO Journal;10/15/98, Vol. 17 Issue 20, p6049 

    A number of splicing factors contain extensive regions that are rich in arginine and serine (RS domains). These domains are thought to facilitate protein—protein interactions that are critical in the regulation of alternative splicing. Using a domain swap strategy, we have tested the...

  • The splicing mutant of the human tumor suppressor protein DFNA5 induces programmed cell death when expressed in the yeast Saccharomyces cerevisiae. Rossom, Sofie Van; De Beeck, Ken Op; Franssens, Vanessa; Swinnen, Erwin; Schepers, Anne; Ghillebert, Ruben; Caldara, Marina; Camp, Guy Van; Winderickx, Joris // Frontiers in Oncology;Jul2012, Vol. 2, p1 

    DFNA5 was first identified as a gene responsible for autosomal dominant deafness. Different mutations were found, but they all resulted in exon 8 skipping during splicing and premature termination of the protein. Later, it became clear that the protein also has a tumor suppression function and...

  • Modulation of p53β and p53γ expression by regulating the alternative splicing of TP53 gene modifies cellular response. Marcel, V; Fernandes, K; Terrier, O; Lane, D P; Bourdon, J-C // Cell Death & Differentiation;Sep2014, Vol. 21 Issue 9, p1377 

    In addition to the tumor suppressor p53 protein, also termed p53α, the TP53 gene produces p53β and p53γ through alternative splicing of exons 9β and 9γ located within TP53 intron 9. Here we report that both TG003, a specific inhibitor of Cdc2-like kinases (Clk) that regulates the...

  • Angiogenesis: What's the alternative? Seton-Rogers, Sarah // Nature Reviews Cancer;Feb2012, Vol. 12 Issue 2, p80 

    The article presents a study that examines the role of Wilms' tumour suppressor gene (WT1)-mediated regulation of splicing factors on alternative splicing of vascular endothelial growth factor A (VEGFA). It details the method of the study in which the researchers conducted a chromatin...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics