TITLE

Fluorescein angiography, optical coherence tomography, and histopathologic findings in a VEGF animal model of retinal angiogenesis

AUTHOR(S)
Arana, Luís; Pinto, Anderson; Chader, Gerald; Barbosa, Jose; Morales, Sabina; Moreira, Ana; Maia, Mauricio; Humayun, Mark
PUB. DATE
October 2012
SOURCE
Graefe's Archive of Clinical & Experimental Ophthalmology;Oct2012, Vol. 250 Issue 10, p1421
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: To establish an animal model of retinal neovascularization using vascular endothelial growth factor (VEGF165) and analyze the model using optical coherence tomography (OCT), fluorescein angiography (FA), and histopathologic evaluation. Methods: Twelve rabbits were divided into groups as follows: group 1 ( n = 3), sham intravitreous injections of 0.1 ml of balanced saline; group 2 ( n = 6), one 10-μg intravitreal injection of VEGF165 on day 0; and group 3 ( n = 3), two 10-μg intravitreal injections of VEGF165, one on day 0 and one on day 7. Follow-up evaluations (days 0, 3, 7, 14, 21, 28) included obtaining fundus color photographs and FA, OCT, and histopathologic examinations. Eyes were enucleated and stained with hematoxylin and eosin (H&E). Results: One injection of VEGF (group 2) was associated with dilatation and tortuosity of the retinal blood vessels that developed within 72 h. Retinal neovascularization was present by day 7 and regressed by day 14. However, even on day 28, the capillaries were still tortuous. Two VEGF injections (group 3) caused increased leakage and neovascularization up to day 14; severe capillary nonperfusion was seen during week 4. At the end of the follow-up period, OCT and histopathologic examination of group 3 showed peripapillary tractional retinal detachments. By day 7, the differences between the retinal thickness seen on OCT in groups 2 and 3 and the group 1 control group were significant ( p < 0.001). The histologic findings showed increased vessel size in groups 2 and 3 by days 14 and 28 compared with the controls. Conclusions: FA, OCT, and histopathologic findings showed that this retinal neovascularization model is efficient, sustainable, and reliable. One injection of VEGF165 created neovascularization that peaked after 1 week; two injections created more intense neovascularization that evolved to retinal detachments after 4 weeks.
ACCESSION #
80730804

 

Related Articles

  • Intravitreal ranibizumab for the treatment of choroidal neovascularization secondary to ocular toxoplasmosis. Shah, Nikunj J.; Shah, Urmi N. // Indian Journal of Ophthalmology;Jul2011, Vol. 59 Issue 4, p318 

    The purpose of the study was to report a case of choroidal neovascularization (CNV) secondary to ocular toxoplasmosis in an 18-year-old female patient. She was treated with a single intravitreal injection of ranibizumab. The CNV resolved as confirmed by fluorescein angiography and optical...

  • Response to fluorescein angiography and OCT. Gallego-Pinazo, Roberto; Díaz-Llopis, M.; Dolz, R.; Martínez-Castillo, S. // Graefe's Archive of Clinical & Experimental Ophthalmology;Mar2011, Vol. 249 Issue 3, p465 

    No abstract available.

  • Therapeutic Effect of Oral Bisphosphonates on Choroidal Neovascularization in the Human Eye. Honda, Shigeru; Nagai, Takayuki; Kondo, Naoshi; Fukuda, Masahide; Kusuhara, Sentaro; Tsukahara, Yasutomo; Negi, Akira // Journal of Ophthalmology;2010, p1 

    Purpose. Choroidal neovascularization (CNV) is often associated with age-related macular degeneration (AMD) and pathological myopia (PM). Bisphosphonates, the drug of choice to treat osteoporosis, have been recently reported to have anti-angiogenic effects. The purpose of this study is to...

  • Retinal Imaging of Infants on Spectral Domain Optical Coherence Tomography. Vinekar, Anand; Mangalesh, Shwetha; Jayadev, Chaitra; Maldonado, Ramiro S.; Bauer, Noel; Toth, Cynthia A. // BioMed Research International;7/6/2015, Vol. 2015, p1 

    Spectral domain coherence tomography (SD OCT) has become an important tool in the management of pediatric retinal diseases. It is a noncontact imaging device that provides detailed assessment of the microanatomy and pathology of the infant retina with a short acquisition time allowing office...

  • Identification of ORT after anti-VEGF treatment for neovascular AMD critical.  // Ocular Surgery News;11/25/2014, Vol. 32 Issue 22, p34 

    The article reports on a study published in the journal "Ophthalmology" that reveals the importance of identification of eyes with outer retinal tubulations after anti-vascular endothelial growth factor therapy for neovascular age-related macular degeneration.

  • Real-world experience may guide intravitreal injection treatments. Dalton, Michelle; Moshfeghi, Andrew // Ophthalmology Times;Mar2015, Vol. 40 Issue 4, p32 

    The article discusses the use of steroid implants in the treatment of patients with diabetic macular edema (DME). Topics covered include the approval of intravitreal injection implants, Ozurdex or dexamethasone from pharmaceutical firm Allergan and fluocinolone acetonide or Iluvien from...

  • Detecting signs of retinal leakage in exudative AMD using Cirrus OCT versus SL SCAN-1, a novel integrated FD-OCT into a common slit lamp. Stehouwer, M.; Verbraak, F.; Schlingemann, R.; Leeuwen, T. // Graefe's Archive of Clinical & Experimental Ophthalmology;Jan2016, Vol. 254 Issue 1, p37 

    Purpose: The purpose is to evaluate the interdevice and interobserver agreements between the SL SCAN-1 (a FD-OCT integrated into a common slit lamp) and a standard stand-alone FD-OCT device (the Cirrus) with regard to the presence or absence of signs of leakage in the retina in patients with...

  • Vitreous inflammatory factors in macular edema with central retinal vein occlusion. Noma, Hidetaka; Funatsu, Hideharu; Harino, Seiyo; Mimura, Tatsuya; Eguchi, Shuichiro; Hori, Sadao // Japanese Journal of Ophthalmology;May2011, Vol. 55 Issue 3, p248 

    Purpose: To investigate the correlation of soluble intercellular adhesion molecule 1 (sICAM-1) and vascular endothelial growth factor (VEGF) with macular edema in patients with central retinal vein occlusion (CRVO). Methods: Twenty-nine patients who had CRVO with macular edema and 16 patients...

  • Tip 2a Ä°diopatik Jukstafoveolar Retinal Telenjektazi'de Ä°ntravitreal Pegabtanip Sodium Tedavisi.  // Retina-Vitreus/Journal of Retina-Vitreous;Sep2010, Vol. 18 Issue 3, p243 

    No abstract available.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics