Bioassay-Guided Isolation, Purification and Partial Characterization of Antimicrobial Compound from Basic Metabolite of Garcinia Kola

Ejele, A. E.; Iwu, I. C.; Enenebeaku, C. K.; Ukiwe, L. N.; Okolue, B. N.
August 2012
Journal of Emerging Trends in Engineering & Applied Sciences;Aug2012, Vol. 3 Issue 4, p668
Academic Journal
Garcinia kola (also called bitter kola) has been identified to possess strong antibacterial and chemotherapeutic activities against several human pathogens such as Staphylococcus aureus, Candida albicans, Coliform bacilli, E. coli, Pseudomonia spp., Salmonella typhi, and Streptococcus spp. although the active principles responsible for these activities are not known with certainty. Therefore the purpose of this study was to purify and characterize the bioactive polyphenolic isolate responsible for the antimicrobial property of the basic metabolite of the plant extract. The bioactive compound, a reddish-brown precipitate, was isolated from the basic metabolite and purified using silica gel packed in a chromatographic column. The various fractions obtained were screened for antimicrobial activity. The fraction that showed the best antimicrobial potential was characterized partially using IR, ¹H- and 13C-NMR spectroscopy. Analyses of the combined spectroscopic data suggested that the compound could be Catechin, p-naphtholbenzein, a-methyl-dl-tyrosine or Naringin. It was concluded that final identification of this polyphenolic compound would be made from the mass spectrum (MS).


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