TITLE

Overexpression of ABCG1 protein attenuates arteriosclerosis and endothelial dysfunction in atherosclerotic rabbits

AUTHOR(S)
Münch, Götz; Bültmann, Andreas; Zhongmin Li; Holthoff, Hans-Peter; Ullrich, Julia; Wagner, Silvia; Ungerer, Martin
PUB. DATE
July 2012
SOURCE
Heart International;Jul2012, Vol. 7 Issue 2, p57
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The ABCG1 protein is centrally involved in reverse cholesterol transport from the vessel wall. Investigation of the effects of ABCG1 overexpression or knockdown in vivo has produced controversial results and strongly depended on the gene intervention model in which it was studied. Therefore, we investigated the effect of local overexpression of human ABCG1 in a novel model of vessel wall-directed adenoviral gene transfer in athero-sclerotic rabbits. We conducted local, vascular-specific gene transfer by adenoviral delivery of human ABCG1 (Ad-ABCG1-GFP) in cholesterol-fed atherosclerotic rabbits in vivo. Endothelial overexpression of ABCG1 markedly reduced atheroprogression (plaque size) and almost blunted vascular inflammation, as shown by markedly reduced macrophage and smooth muscle cell invasion into the vascular wall. Also endothelial function, as determined by vascular ultrasound in vivo, was improved in rabbits after gene transfer with Ad-ABCG1-GFP . Therefore, both earlier and later stages of atherosclerosis were improved in this model of somatic gene transfer into the vessel wall. In contrast to results in transgenic mice, over-expression of ABCG1 by somatic gene transfer to the atherosclerotic vessel wall results in a significant improvement of plaque morphology and composition, and of vascular function in vivo.
ACCESSION #
80417007

 

Related Articles

  • Imaging expression of adenoviral HSV1-tk suicide gene transfer using the nucleoside analogue FIRU. Nanda, Dharmin; de Jong, Marion; Vogels, Ronald; Havenga, Menzo; Driesse, Maarten; Bakker, Willem; Bijster, Magda; Avezaat, Cees; Cox, Peter; Morin, Kevin; Naimi, Ebrahim; Knaus, Edward; Wiebe, Leonard; Smitt, Sillevis // European Journal of Nuclear Medicine & Molecular Imaging;2002, Vol. 29 Issue 7, p939 

    Substrates for monitoring HSV1-tk gene expression include uracil and acycloguanosine derivatives. The most commonly used uracil derivative to monitor HSV1-tk gene transfer is 1-(2-fluoro-2-deoxy-β-D-arabinofuranosyl)-5-[*I]iodouracil (fialuridine; I*-FIAU), where the asterisk denotes any of...

  • Transduction of the contralateral ear after adenovirus-mediated cochlear gene transfer. Stöver, T; Yagi, M; Raphael, Y // Gene Therapy;Mar2000, Vol. 7 Issue 5, p377 

    Cochlear gene transfer is a promising new approach for inner ear therapy. Previous studies have demonstrated hair cell protection with cochlear gene transfer not only in the inoculated, but also in the uninoculated ear. To characterize the kinetics of viral spread, we investigated the extent of...

  • Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Betz, O B; Betz, V M; Nazarian, A; Egermann, M; Gerstenfeld, L C; Einhorn, T A; Vrahas, M S; Bouxsein, M L; Evans, C H // Gene Therapy;Jul2007, Vol. 14 Issue 13, p1039 

    The direct, local, administration of adenovirus carrying human BMP-2 cDNA (Ad.BMP-2) heals critical-sized femoral bone defects in rabbit and rat models. However, the outcome is suboptimal and the technology needs to provide a more reliable and uniform outcome. To this end, we investigated...

  • Virus-mediated Transfer of Foreign DNA into Taste Receptor Cells. Stone, Leslie M.; Wilcox, Christine L.; Kinnamon, Sue C. // Chemical Senses;Nov2002, Vol. 27 Issue 9, p779 

    Foreign genes can be transferred into taste cells via adenoviral vectors. The present study was undertaken to characterize the subpopulation of taste cells that are susceptible to adenovirus infection and to determine whether another viral vector, derived from herpes simplex 1 (HSV-1), infects...

  • Cross-talk between phosphorylation and SUMOylation regulates transforming activities of an adenoviral oncoprotein. Wimmer, P; Blanchette, P; Schreiner, S; Ching, W; Groitl, P; Berscheminski, J; Branton, P E; Will, H; Dobner, T // Oncogene;3/28/2013, Vol. 32 Issue 13, p1626 

    Since the discovery of post-translational modification (PTM) by the small ubiquitin-related modifiers (SUMOs), a multitude of proteins have been described to be reversibly modified, resulting in the alteration of several cellular pathways. Interestingly, various pathogens gain access to this...

  • Transient foamy virus vector production by adenovirus vectors. Russell, R.A.; Vassaux, G.; Martin-Duque, P.; McClure, M.O. // Gene Therapy;Feb2004, Vol. 11 Issue 3, p310 

    The genome of the prototype foamy virus (PFV) has been introduced into an adenoviral/PFV hybrid vector and tested for stable in vitro gene transfer. Three different adenoviruses are used to encode: (i) the PFV structural genes gag and pol (Ad-GagPol?PacI); (ii) the PFV structural gene env...

  • Temporally regulated expression patterns following in utero adenovirus-mediated gene transfer. Schachtner, S K; Buck, C A; Bergelson, J M; Baldwin, H S // Gene Therapy;Jul99, Vol. 6 Issue 7, p1249 

    Developmental patterns of gene expression were determined following intravascular administration of adenovirus in utero, during sequential stages of murine development. Replication-deficient adenovirus (AdCMV.LacZ) was injected into yolk sac vessels of mouse embryos 12, 13, 15 and 18 days...

  • From the editors.  // Nature Reviews Genetics;May2011, Vol. 12 Issue 5, p295 

    An introduction is presented in which the editors discuss various reports within the issue on topics related to gene-based therapies, including adeno-associated virus vectors, co-implementation of cell-based delivery and gene-transfer technologies, and RNA interference (RNAi).

  • Transient increase in lung epithelial tight junction permeability: an additional mechanism for enhancement of lung transgene expression by perfluorochemical liquids. Weiss, Daniel J.; Beckett, Travis; Bonneau, Laura; Young, Jadrien; Kolls, Jay K.; Wang, Guoshun // Molecular Therapy;Dec2003, Vol. 8 Issue 6, p927 

    Intratracheal instillation of perfluorochemical (PFC) liquids enhances lung epithelial transgene expression by improved vector propulsion throughout lung airways. We now demonstrate that PFC liquids also facilitate gene transfer by increasing transepithelial permeability. Apical application of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics