Influencia de las vías de inmunización mucosales sobre la protección contra herpes simple tipo 2 con el AFCo1 como adyuvante

Cabrera, Osmir; Cuello, Maribel; Thörn, Karolina; Lindqvist, Madelene; Lastre, Miriam; González, Elizabeth; Zayas, Caridad; Balboa, Julio; Romeo, Belkis; Sarandi, Ali M.; Pérez, Oliver
September 2011
Revista VacciMonitor (Vacunología y Temas Afines);sep-dic2011, Vol. 20 Issue 3, p14
Academic Journal
Mucosal vaccines are a promising strategy to induce mucosal protection. Herpes simplex virus type 2 is the commonest pathogens in the human transmitted by exposure at the genital mucosal surfaces. Many vaccine candidates against this pathogen have been evaluated; but they have not been effective, and neither a prophylactic nor a therapeutic vaccine has been yet obtained. The gD2 is a recombinant glycoprotein and it is reported as one of the antigens of importance for vaccine against this virus. There is the cochleate (AFCol) derived from proteoliposome of Neisseria meningitidis serogroup B. This cochleate has shown potentialities as adjuvant for several immunization routes. The objective of this study was to evaluate the protection induced in mice by the AFCo1-gD2 administered by different mucosal routes. Female mice C57BL/6 were used and immunized by: intranasal (IN), intravaginal (IVag), or intrarectal (IR) routes with AFCo1-gD2 or gD2 alone. The anti-gD2 IgG and specific cellular proliferation were determined and the viral replication in vaginal wash, the signs of disease and the protection against the viral challenge, were measured too. A significant anti-gD2 IgG response was obtained by all routes, although the IN route showed the highest values. Cellular proliferation was observed in cells of animals immunized IN and IVag route; but not by IR route. In addition, a higher protection (100%) in the animals Immunized with AFCol -gD2 by IN route was observed. In conclusion the intranasal is the most promising route in the protection induction against this viral challenge.


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