TITLE

The First Exon of the Rat Aldolase C Gene Is Essential for Restoring the Chromatin Structure in Transgenic Mice

AUTHOR(S)
Arai, Yuji; Sugama, Takako; Hashido, Kazuo; Ohishi, Sachiko; Mukai, Tsunehiro
PUB. DATE
January 1997
SOURCE
Journal of Biochemistry;1997, Vol. 122 Issue 5, p927
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A 13-kb fragment of the rat aldolase C gene contains sufficient information for gene expression. Transgenic mice carrying the 13-kb fragment showed restoration of chromatin structure and tissue-specific, copy number-dependent expression. To localize the regulatory elements responsible for restoring chromatin structure, several mutated constructs were used to produce transgenic mice. Three activities were examined: recreation of DNase hypersensitive sites, restoration of methylation status, and copy number-dependent expression. Deletions of the 3′-flanking region did not affect those activities. Deletion of seven introns affected the mRNA levels but not the restoration of the chromatin structure. The insertion of the LacZ gene into the first exon of the transgene interfered with both the restoration of the chromatin structure and the copy number-dependent expression in transgenic mice. DNase I footprinting assays revealed that brain-specific factors bind to the sequence disrupted by the LacZ insertion. These results suggest that the sequence in the first exon is essential for restoring the chromatin structure of the rat aldolase C gene.
ACCESSION #
80076691

 

Related Articles

  • LEISURE.  // Indianapolis Monthly;Dec2010, Vol. 34 Issue 4, p70 

    The article focuses on the leisure shops located in Indiana. It highlights the services offered by several leisure shops including the bar trivia night at the Elbow Room, the puppet show in Peewinkle's Puppet Studio, and mascot shows in Rowdie. It also features the leisure shops Indianapolis...

  • Big ol' gay Christmas. Hanashiro, Marvin // Gay & Lesbian Times;11/30/2006, Issue 988, p52 

    The article features places in San Diego, California, to spend and enjoy the holidays. On December 13 and 20, 2006, Urban Mo's will present a "Dreamgirls Christmas show." On December 19, 2006, the establishment will feature a "Christmas Movie Night." At Detour, one can enjoy some dancing on...

  • MARQUEE. Block, Katie // New Orleans;Jun2004, Vol. 38 Issue 9, p10 

    The article discusses the recreation facilities of New Orleans, Louisiana. If the thought of a live outdoor concert is music to your cars, attend the" Thursdays at Twilight" Garden Concert Series held throughout June at the Pavilion of the Two Sisters in New Orleans City Park. Singer Patti Adams...

  • How to Do More With Less. Roberts, Rachel // Parks & Recreation;Aug2004, Vol. 39 Issue 8, p15 

    Explores articles and topics related to parks and recreation in the U.S. as of August 2004.

  • By the High Mercy of Santa Baba. Gibbons, John // America;8/6/1932, Vol. 47 Issue 18, p419 

    The author accounts on the visit he and his family made at the Alexandra Palace in London, England.

  • THE BIG ISSUE.  // Crain's Cleveland Business;11/29/2004, Vol. 25 Issue 48, p11 

    The article presents information on some places for recreational tour in Cleveland, Ohio.

  • Farklı bölgelere göre 11-15 yaÅŸ grubu çocukların rekreatif faaliyetlerinin karşılaÅŸtırılması. Pala, Adem; KolayıŞ, Hakan // International Journal of Human Sciences;Jan-Jun2012, Vol. 9 Issue 1, p1007 

    The purpose of this study is to compare the recreative activities of 11-15 age group depending on different Regions. In this respect, 227 children from Southeastern part of Turkey and 262 children from Marmara region of Turkey participated in this study in a volunteered way. The questionnaire...

  • Enduring Involvement, Situational Involvement, and Flow in Leisure and Non-leisure Activities. Havitz, Mark E.; Mannell, Roger C. // Journal of Leisure Research;2005 2nd Quarter, Vol. 37 Issue 2, p152 

    This research was conducted to investigate relationships between enduring involvement (EI), situational involvement (SI), and flow. It was hypothesized that the constructs would be related and that SI would mediate relationships between EI and flow. In addition, the relationships were examined...

  • Summer Activities For Your Child.  // Ebony;Jun2005, Vol. 60 Issue 8, p118 

    Presents information summer recreational activities for children. Importance of summertime activities; Social benefits associated with summer activities; Tips for parents to encourage their children to participate in recreational activities.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics