TITLE

Gossypol Inhibits Electron Transport and Stimulates ROS Generation in Yarrowia lipolytica Mitochondria

AUTHOR(S)
Arinbasarova, Anna Yu.; Medentsev, Alexander G.; Krupyanko, Vladimir I.
PUB. DATE
January 2012
SOURCE
Open Biochemistry Journal;2012, Vol. 6, p11
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This work studied the effect of gossypol on the mitochondrial respiratory chain of Yarrowia lipolytica. The compound was shown to inhibit mitochondrial electron transfer and stimulate generation of reactive oxygen species. The inhibition kinetics in oxidation of various substrates (NADH, succinate, μ-glycerophosphate and pyruvate + malate) by isolated mitochondria was investigated. Analysis of the kinetic parameters showed gossypol to inhibit two fragments of the mitochondrial electron transfer chain: a) between coenzyme Q and cytochrome b with KIIIi of 118.3 μM (inhibition by the noncompetitive type), and b) at the level of exogenous NADH dehydrogenase with K Ii of 17.2 μM (inhibition by the mixed type)
ACCESSION #
80036957

 

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