Macelignan Inhibits Histamine Release and Inflammatory Mediator Production in Activated Rat Basophilic Leukemia Mast Cells

Han, Young; Kim, Myung-Suk; Hwang, Jae-Kwan
October 2012
Inflammation;Oct2012, Vol. 35 Issue 5, p1723
Academic Journal
Type I allergy is characterized by the release of granule-associated mediators, lipid-derived substances, cytokines, and chemokines by activated mast cells. To evaluate the anti-allergic effects of macelignan isolated from Myristica fragrans Houtt., we determined its ability to inhibit calcium (Ca) influx, degranulation, and inflammatory mediator production in RBL-2 H3 cells stimulated with A23187 and phorbol 12-myristate 13-acetate. Macelignan inhibited Ca influx and the secretion of β-hexosaminidase, histamine, prostaglandin E, and leukotriene C; decreased mRNA levels of cyclooxygenase-2, 5-lipoxygenase, interleukin-4 (IL-4), IL-13, and tumor necrosis factor-α; and attenuated phosphorylation of Akt and the mitogen-activated protein kinases extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. These results indicate the potential of macelignan as a type I allergy treatment.


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