TITLE

Distribution of fluorescence decay times for 1-anilinonaphthalene-8-sulfonate in human oxyhemoglobin A solution

AUTHOR(S)
Drazdou, A.; Sobchuk, A.; Syakhovich, V.; Bokut, O.; Kvasyuk, E.; Bushuk, B.; Bokut, S.
PUB. DATE
July 2012
SOURCE
Journal of Applied Spectroscopy;Jul2012, Vol. 79 Issue 3, p437
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We have studied complex formation between molecules of the fluorescent probe 1-anilinonaphthalene-8-sulfonate (1,8-ANS) and the major form (A) and a minor form (A) of hemoglobin. The contribution of the longlived component f to the kinetic curves for fluorescence decay in HbA solutions is 0.021-0.036, which indicates a dramatic decrease (compared with HbA) in the accessibility of the central cavity of HbA for binding 1,8-ANS. Disappearance of the long-lived component f in the fluorescence decay kinetics of 1,8-ANS in HbA solutions in the presence of inositol hexaphosphate (IHP) suggests that the regulatory region of HbA1b is completely inaccessible for interaction both with the negatively charged molecules of the probe and with natural regulators of the transport function for this form of the heme protein.
ACCESSION #
79824127

 

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