TITLE

Specific binding of tubeimoside-2 with proteins in hepatocarcinoma HepG2 cells: investigation by molecular spectroscopy

AUTHOR(S)
Yang, Sun; Shi-Sheng, Sun; Ying-Yong, Zhao; Jun, Fan
PUB. DATE
July 2012
SOURCE
Journal of Applied Spectroscopy;Jul2012, Vol. 79 Issue 3, p416
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In this study, we compared different binding interactions of TBMS2 with proteins both in hepatocarcinoma HepG2 cells and in normal embryo hepatic L02 cells by using fluorescence, absorption, and CD spectroscopy. The fluorescence data revealed that the fluorescence intensity of proteins in the HepG2 and L02 cells decreased in the presence of TBMS2 by 30.79% and 12.01%, respectively. Binding constants and thermodynamic parameters were obtained for systems of TBMS2 with the two kinds of cell proteins. The results indicated that HepG2 cell proteins had a higher TBMS2 binding activity than those in the L02 cells. Analysis of the TBMS2 cytotoxic activities showed that TBMS2 could selectively induce apoptosis of HepG2 cells by binding to them, while its apoptotic effect on L02 cells was relatively weaker.
ACCESSION #
79824118

 

Related Articles

  • Apoptosis sensitizers enhance cytotoxicity in hepatoblastoma cells. Lieber, Justus; Ellerkamp, Verena; Wenz, Julia; Kirchner, Bettina; Warmann, Steven; Fuchs, Jörg; Armeanu-Ebinger, Sorin // Pediatric Surgery International;Feb2012, Vol. 28 Issue 2, p149 

    Purpose: Drug resistance remains a major challenge for the treatment of high-risk hepatoblastoma (HB). To enhance effectiveness of chemotherapy we modulate apoptosis in HB cells in vitro. Methods: Viability was monitored in HB cells (HuH6, HepT1) and fibroblasts in monolayer and spheroid...

  • Melatonin induces transcriptional regulation of Bim by FoxO3a in HepG2 cells. Carbajo-Pescador, S; Steinmetz, C; Kashyap, A; Lorenz, S; Mauriz, J L; Heise, M; Galle, P R; González-Gallego, J; Strand, S // British Journal of Cancer;2/5/2013, Vol. 108 Issue 2, p442 

    Background:Melatonin induces apoptosis in many different cancer cell lines, including hepatocellular carcinoma cells. However, the responsible pathways have not been clearly elucidated. A member of the forkhead transcription factors' family, FoxO3a, has been implicated in the expression of the...

  • Novel palladium(II) complexes containing a sulfur ligand: structure and biological activity on HeLa cells. Gao, EnJun; Guan, Feng; Gao, XiaNan; Zhu, MingChang; Liu, Lei; Wang, ChuanSheng; Zhang, WanZhong; Sun, YaGuang // Journal of Biological Inorganic Chemistry;Feb2012, Vol. 17 Issue 2, p263 

    Two novel palladium(II) complexes with a thiosalicylic acid (HSCHCOH) ligand, with the formulas [Pd(TSA)(L)]· mHO (TSA is thiosalicylic acid; in complex 1, L is 1,10-phenanthroline and m = 1; in complex 2, L is 2,2′-bipyridine and m = 2), have been synthesized and characterized. The...

  • Role of RNase L in apoptosis induced by 1-(3- C-ethynyl-β- d- ribo-pentofuranosyl)cytosine. Naito, Tomoharu; Yokogawa, Tatsushi; Takatori, Satoshi; Goda, Kazato; Hiramoto, Akiko; Sato, Akira; Kitade, Yukio; Sasaki, Takuma; Matsuda, Akira; Fukushima, Masakazu; Wataya, Yusuke; Kim, Hye-Sook // Cancer Chemotherapy & Pharmacology;Apr2009, Vol. 63 Issue 5, p837 

    1-(3- C-Ethynyl-β- d- ribo-pentofuranosyl)cytosine (ECyd), a ribonucleoside analog, has a potent cytotoxic activity against cancer cells. The present studies have been performed to elucidate the overall mechanisms of ECyd-induced apoptotic cell death. Cultured cells of mouse mammary carcinoma...

  • Lichen Secondary Metabolites in Flavocetraria cucullata Exhibit Anti-Cancer Effects on Human Cancer Cells through the Induction of Apoptosis and Suppression of Tumorigenic Potentials. Nguyen, Thanh Thi; Yoon, Somy; Yang, Yi; Lee, Ho-Bin; Oh, Soonok; Jeong, Min-Hye; Kim, Jong-Jin; Yee, Sung-Tae; Crişan, Florin; Moon, Cheol; Lee, Kwang Youl; Kim, Kyung Keun; Hur, Jae-Seoun; Kim, Hangun // PLoS ONE;Oct2014, Vol. 9 Issue 10, p1 

    Lichens are symbiotic organisms which produce distinct secondary metabolic products. In the present study, we tested the cytotoxic activity of 17 lichen species against several human cancer cells and further investigated the molecular mechanisms underlying their anti-cancer activity. We found...

  • Cytotoxity Evaluation of Docetaxel Nanoparticles by Culturing HepG2 Carcinoma Cell Lines. Shokrzadeh, Mohammad; Ebrahimnejad, Pedram; Omidi, Mahmood; Shadboorestan, Amir; Zalzar, Zavosh // Journal of Mazandaran University of Medical Sciences (JMUMS);Jul2012, Vol. 22 Issue 90, p2 

    Background and purpose: Human hepatocellular carcinoma (HCC) is one of the most common causes of death in the world. Targeted drug delivery to cells, tissues or specialized receptor cells is an advanced technology in treatment of HCC. The purpose of this study was to investigate the cytotoxicity...

  • Quercetin - A Flavonoid Compound from Sarcopyramis bodinieri var delicate with Potential Apoptotic Activity in HepG2 Liver Cancer Cells. Weifeng Huang; Chunpeng Wan; Shouran Zhou // Tropical Journal of Pharmaceutical Research;Aug2013, Vol. 12 Issue 4, p529 

    Purpose: To isolate and identify the cytotoxicity of the constituents of Sarcopyramis bodinieri var. delicate. Methods: S. bodinieri var. delicate was extracted with hydrochloric acid-methanol and fractionated with ethyl acetate further. The chemical constituents of the ethyl acetate fraction...

  • Resveratrol caused apoptosis in QGY-7701 cells. J.-P., LI // European Review for Medical & Pharmacological Sciences;Sep2015, Vol. 19 Issue 17, p3303 

    OBJECTIVE: This study aims to evaluate the cytotoxicity of resveratrol in QGY7701 cells using a cell viability assay and determine genetic damages after 24-h exposure to concentrations of 6, 12, and 24 µmol·L-1. MATERIALS AND METHODS: Cell viability and genetic damages were determined...

  • PX-12 inhibits the growth of hepatocelluar carcinoma by inducing S-phase arrest, ROS-dependent apoptosis and enhances 5-FU cytotoxicity. Guang-Zhen Li; Hui-Fang Liang; Bo Liao; Lei Zhang; Ya-An Ni; Hong-Hao Zhou; Er-Lei Zhang; Bi-Xiang Zhang; Xiao-Ping Chen // American Journal of Translational Research;2015, Vol. 7 Issue 9, p1528 

    Background: 1-methylpropyl 2-imidazolyl disulfide (PX-12), a thioredoxin 1 (Trx1) inhibitor, has been investigated in a number of ancers, but its effectiveness in the treatment of hepatocellular carcinoma (HCC) has not been reported. PX-12 has generated considerable interest in its use in a...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics