TITLE

Biodegradation of Poly(β-hydroxybutyrate) by a novel isolate of Streptomyces bangladeshensis 77T-4

AUTHOR(S)
Hsu, Ke-Jen; Tseng, Min; Don, Trong-Ming; Yang, Mei-Kwei
PUB. DATE
July 2012
SOURCE
Botanical Studies;Jul2012, Vol. 53 Issue 3, p307
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A thermophilic Streptomyces bangladeshensis isolate designated 77T-4 that can degrade poly(β-hydroxybutyrate) (PHB) was isolated from a soil sample in Taiwan. Isolate 77T-4 grew well in urea fructose oatmeal medium and produced a clear zone around the colony when grown on an agar plate containing emulsified PHB, indicating the presence of an extracellular PHB depolymerases. A PHB-degrading enzyme was purified to homogeneity from the culture supernatant. The molecular weight of the PHB-degrading enzyme was estimated to be approximately 40 kDa. A small portion of the N-terminal sequence (Ala-Val-Pro- Leu-Thr-Arg ) of the purified enzyme was determined and found to share a significant homology with that of the PHB depolymerase of Streptomyces sp. MG.
ACCESSION #
79746015

 

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