TITLE

Sugar uptake analysis of suspension Arabidopsis, tobacco, and rice cells in various media using an FT-IR/ATR method

AUTHOR(S)
Suehara, Ken-ichiro; Kameoka, Takaharu; Hashimoto, Atsushi
PUB. DATE
October 2012
SOURCE
Bioprocess & Biosystems Engineering;Oct2012, Vol. 35 Issue 8, p1259
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The kinetic behavior of the sugar uptake phenomena of a suspension of Arabidopsis cells was investigated by mid-infrared spectroscopy using Fourier transform infrared spectrometers and attenuated total reflection techniques. The kinetic behavior of the cell growth was also studied and the growth and the sugar uptake behaviors were discussed for three typical plant cells (Arabidopsis, TBY-2, and rice cells). The cell growth rate and the lag period were influenced by not only the types of the plant cells, but also the sugar species used as the carbon source. The characteristics of the sugar uptake behavior were clarified based on the difference in the three types of plant cells. The cell growth and the sugar uptake progressed at approximately the same time in the TBY-2 cells. In the rice cells, the sugar uptake rate was relatively lower than that of the others. On the other hand, the sugar uptake of the Arabidopsis cells started before the cell growth. Furthermore, glucose as the carbon source of the Arabidopsis cell cultivation seems to significantly influence the sugar metabolism. Glucose had a significant influence on the sugar metabolism of the other sugar under the conditions for the mixture of glucose and the other sugar. The characteristics of the sugar uptake phenomena based on the cell growth stage was typical for each plant cell except for some sugars, such as galactose and trehalose, and the behavior of the total sugar uptake had not changed. These results suggested that the cell growth and the sugar uptake in the plant cell cultivation processes may be controlled by the combined supply of the sugar species as the carbon source. The detailed data for plant cell cultivation using each sugar obtained in this study would be useful for bioscience research and for cultivation process control using various sugars, for example, purified or sugar mixtures formed from biomass materials.
ACCESSION #
79615382

 

Related Articles

  • Investigating the heterogeneity of cell growth in microbial colonies by FTIR microspectroscopy. Thi, N. A. Ngo; Naumann, D. // Analytical & Bioanalytical Chemistry;Mar2007, Vol. 387 Issue 5, p1769 

    Microorganisms rarely occur as individual cells in nature and are, instead, organized in complex multicellular communities such as colonies, fruiting bodies, or biofilms. Interest in the natural microbial life-style has increased during the last decade and a whole plethora of techniques has been...

  • FT-IR spectrometers perform environmental monitoring. Russwurm, George M.; Childers, Jeffrey W. // Laser Focus World;Apr95, Vol. 31 Issue 4, p79 

    Reports on the use of the Fourier-transform infrared (FT-IR) long-path spectrometer system to measure atmospheric gas concentrations. Features of the system Components of an FT-IR long-path monitoring instrument; Data analysis using the FT-IR interferogram.

  • In-situ chemical analyses of trans-polyisoprene by histochemical staining and Fourier transform infrared microspectroscopy in a rubber-producing plant, Eucommia ulmoides Oliver. Bamba, Takeshi; Fukusaki, Ei-ichiro; Nakazawa, Yoshihisa; Kobayashi, Akio // Planta;Oct2002, Vol. 215 Issue 6, p934 

    The localization of polyisoprene in young stem tissues of Eucommia ulmoides Oliver was investigated by histochemical staining and Fourier transform infrared (FT–IR) microspectroscopy. The fibrous structures were stained with Oil Red O. FT–IR microspectroscopic analysis proved that...

  • Separation characteristics of lignin from Eucalyptus camaldulensis lignincelluloses for biomedical cellulose. Wanxi PENG; Lansheng WANG; Minglong ZHANG; Zhi LIN // Pakistan Journal of Pharmaceutical Sciences;May2014 Supplement, Vol. 27, p723 

    The separation characteristics of Eucalyptus camaldulensis biomass cell wall were investigated and analyzed by FTIR, UV and XRD. The result showed that the crystallinity of untreated sample were lower than ones of treated samples at 10°C, 20°C and 30°C. Effect of temperature was very...

  • Changes in carotenoid content and distribution in living plant tissue can be observed and mapped in situ using NIR-FT-Raman spectroscopy. Baranski, Rafal; Baranska, Malgorzata; Schulz, Hartwig // Planta;Dec2005, Vol. 222 Issue 3, p448 

    Near-infrared (NIR) excited Fourier transform (FT) Raman spectroscopy has been applied for in situ analysis of carotenoids in living plant samples. Pelargonium x hortorum leaf has been mapped using a Raman mapping technique to illustrate heterogeneous distribution of carotenoids. Mapping has...

  • On the nature of vibrational bands in the FTIR spectra of medicinal plant leaves. Konwar, Mitali; Baruah, G. D. // Archives of Applied Science Research;2011, Vol. 3 Issue 1, p214 

    Fifteen samples of dried leaves of medicinal values from different plants are selected for study of their FTIR spectra. The main purpose of their study is to observe the salient features exhibited by the IR spectra.All the fourteen bands originating from different groups (N-H, O-H, C-H, C=O, C=C...

  • ERRATUM.  // Journal of the American Oil Chemists' Society (JAOCS);Jul2004, Vol. 81 Issue 7, p725 

    Presents a correction to the article "New FTIR Method for the Determination of FFA in Oils," by Ahmed Al-Alawi, Frederick R. Van de Voort and Jacqueline Sedman, which appeared in the 2004 issue of the "Journal of the American Oil Chemists' Society."

  • FTIR spectra of ammonia clusters in noble gas matrices. Süzer, Sefik; Andrews, Lester // Journal of Chemical Physics;11/1/1987, Vol. 87 Issue 9, p5131 

    FTIR spectra of ammonia have been studied from 200 to 5200 cm-1 over a wide range of concentration and temperature conditions in solid neon, argon, and nitrogen matrices. Dimer bands appear between monomer and higher aggregate absorptions and exhibit intermediate growth behavior on sample...

  • A bright future for synchrotron imaging. Petibois, C.; Déléris, G.; Piccinini, M.; Cestelli-Guidi, M.; Marcelli, A. // Nature Photonics;Apr2009, Vol. 3 Issue 4, p179 

    A letter to the editor is presented discussing Fourier transform infrared (FTIR) microscopy, and the new analytic techniques being made possible by developments in high-brightness synchrotron sources equipped with focal plane array (FPA) detectors.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics