Sugar uptake analysis of suspension Arabidopsis, tobacco, and rice cells in various media using an FT-IR/ATR method

Suehara, Ken-ichiro; Kameoka, Takaharu; Hashimoto, Atsushi
October 2012
Bioprocess & Biosystems Engineering;Oct2012, Vol. 35 Issue 8, p1259
Academic Journal
The kinetic behavior of the sugar uptake phenomena of a suspension of Arabidopsis cells was investigated by mid-infrared spectroscopy using Fourier transform infrared spectrometers and attenuated total reflection techniques. The kinetic behavior of the cell growth was also studied and the growth and the sugar uptake behaviors were discussed for three typical plant cells (Arabidopsis, TBY-2, and rice cells). The cell growth rate and the lag period were influenced by not only the types of the plant cells, but also the sugar species used as the carbon source. The characteristics of the sugar uptake behavior were clarified based on the difference in the three types of plant cells. The cell growth and the sugar uptake progressed at approximately the same time in the TBY-2 cells. In the rice cells, the sugar uptake rate was relatively lower than that of the others. On the other hand, the sugar uptake of the Arabidopsis cells started before the cell growth. Furthermore, glucose as the carbon source of the Arabidopsis cell cultivation seems to significantly influence the sugar metabolism. Glucose had a significant influence on the sugar metabolism of the other sugar under the conditions for the mixture of glucose and the other sugar. The characteristics of the sugar uptake phenomena based on the cell growth stage was typical for each plant cell except for some sugars, such as galactose and trehalose, and the behavior of the total sugar uptake had not changed. These results suggested that the cell growth and the sugar uptake in the plant cell cultivation processes may be controlled by the combined supply of the sugar species as the carbon source. The detailed data for plant cell cultivation using each sugar obtained in this study would be useful for bioscience research and for cultivation process control using various sugars, for example, purified or sugar mixtures formed from biomass materials.


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