A Rat Model of Central Venous Catheter to Study Establishment of Long-Term Bacterial Biofilm and Related Acute and Chronic Infections

Chauhan, Ashwini; Lebeaux, David; Decante, Benoit; Kriegel, Irene; Escande, Marie-Christine; Ghigo, Jean-Marc; Beloin, Christophe
May 2012
PLoS ONE;May2012, Vol. 7 Issue 5, p1
Academic Journal
Formation of resilient biofilms on medical devices colonized by pathogenic microorganisms is a major cause of health-care associated infection. While in vitro biofilm analyses led to promising anti-biofilm approaches, little is known about their translation to in vivo situations and on host contribution to the in vivo dynamics of infections on medical devices. Here we have developed an in vivo model of long-term bacterial biofilm infections in a pediatric totally implantable venous access port (TIVAP) surgically placed in adult rats. Using non-invasive and quantitative bioluminescence, we studied TIVAP contamination by clinically relevant pathogens, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis, and we demonstrated that TIVAP bacterial populations display typical biofilm phenotypes. In our study, we showed that immunocompetent rats were able to control the colonization and clear the bloodstream infection except for up to 30% that suffered systemic infection and death whereas none of the immunosuppressed rats survived the infection. Besides, we mimicked some clinically relevant TIVAP associated complications such as port-pocket infection and hematogenous route of colonization. Finally, by assessing an optimized antibiotic lock therapy, we established that our in vivo model enables to assess innovative therapeutic strategies against bacterial biofilm infections.


Related Articles

  • Heparin-Binding Motifs and Biofilm Formation by Candida albicans. Green, Julianne V.; Orsborn, Kris I.; Zhang, Minlu; Tan, Queenie K. G.; Greis, Kenneth D.; Porollo, Alexey; Andes, David R.; Long Lu, Jason; Hostetter, Margaret K. // Journal of Infectious Diseases;Nov2013, Vol. 208 Issue 10, p1695 

    Candida albicans is a leading pathogen in infections of central venous catheters, which are frequently infused with heparin. Binding of C. albicans to medically relevant concentrations of soluble and plate-bound heparin was demonstrable by confocal microscopy and enzyme-linked immunosorbent...

  • Research & Markets: Egypt Drug Delivery Devices Investment Opportunities, Analysis and Forecasts to 2017.  // Biomedical Market Newsletter;6/30/2011, p504 

    The article presents an analysis and forecast of the drug delivery devices investment opportunities in Egypt up to the year 2017. It provides information on the value, volume and average price data for various segments within four market categories including central venous catheters, infusion...

  • Research & Markets: Europe Drug Delivery Devices Market Outlook to 2017 - Report Out Now.  // Biomedical Market Newsletter;6/30/2011, p534 

    The article presents a market outlook for the drug delivery devices sector in Europe up to the year 2017. It provides value data for all market categoeis that include central venous catheters, infusion systems and needle free injections. It offers details on the company shares and distribution...

  • Improved diagnosis of central venous catheter-related bloodstream infections using the HB&L UROQUATTROâ„¢ system. Fontana, C.; Favaro, M.; Bossa, M.; Minelli, S.; Altieri, A.; Pelliccioni, M.; Falcione, F.; Traglia, L.; Cicchetti, O.; Favalli, C. // European Journal of Clinical Microbiology & Infectious Diseases;Nov2012, Vol. 31 Issue 11, p3139 

    The diagnosis of catheter-related bloodstream infections (CRBSIs) in febrile patients with indwelling central venous catheters (CVCs) needs improvement. To diagnose CRBSIs more efficiently, we have developed a novel culture approach using the catheter tips removed from febrile patients. CVCs and...

  • Catheter associated bloodstream infection caused by R. radiobacter. Sood, S.; Nerurkar, V.; Malvankar, S. // Indian Journal of Medical Microbiology;Jan2010, Vol. 28 Issue 1, p62 

    Rhizobium radiobacter is a gram negative bacillus that is infrequently recognized in clinical specimens but is emerging as an opportunistic human pathogen. Infections due to Rhizobium radiobacter are strongly related to the presence of foreign plastic material and effective treatment often...

  • Impact of Anesthesia Protocols on In Vivo Bioluminescent Bacteria Imaging Results. Chuzel, Thomas; Sanchez, Violette; Vandamme, Marc; Martin, Stéphane; Flety, Odile; Pager, Aurélie; Chabanel, Christophe; Magnier, Luc; Foskolos, Marie; Petit, Océane; Rokbi, Bachra; Chereul, Emmanuel // PLoS ONE;7/24/2015, Vol. 10 Issue 7, p1 

    Infectious murine models greatly benefit from optical imaging using bioluminescent bacteria to non-invasively and repeatedly follow in vivo bacterial infection. In this context, one of the most critical parameters is the bioluminescence sensitivity to reliably detect the smallest number of...

  • Candidaemia in Patients with an Inserted Medical Device. Cauda, Roberto // Drugs;2009 Supplement, Vol. 69, p33 

    The importance of medical devices as a source of infection has been increasingly appreciated. Candida spp. are responsible for a significantly increasing number of device-related infections, particularly those involving the bloodstream and urinary tract. A wide range of devices may be colonized...

  • Care provision: lessons learnt from near and far. Nickolson, Jackie // British Journal of Nursing;11/22/2012 Supplement, pS3 

    The article discusses various reports which are published in the issue, including one by Thaís Queiroz Santolim on vessel health and preservation, one by Nicola York on central venous access devices and one by Sophie Harnage on the elimination of central-line associated bloodstream infections...

  • Evidence-based consensus on the insertion of central venous access devices. Baines, D. B. // BJA: The British Journal of Anaesthesia;Feb2014, Vol. 112 Issue 2, p382 

    No abstract available.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics