TITLE

Cytochemical Localization of Calcium and Ca2+-ATPase Activity in Plant Cells under Chilling Stress: a Comparative Study between the Chilling-Sensitive Maize and the Chilling-Insensitive Winter Wheat

AUTHOR(S)
Jian, Ling-cheng; Li, Ji-hong; Chen, Wen-Ping; Li, Paul H.; Ahlstrand, Gilbert G.
PUB. DATE
October 1999
SOURCE
Plant & Cell Physiology;Oct1999, Vol. 40 Issue 10, p1061
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Electron microscopic observations revealed that electron-dense antimonate Ca2+ deposits were mostly localized in the vacuole and the intercellular space in both maize and winter wheat when their seedlings were grown at 25°C. The reaction products—cerium phosphate deposits of Ca2+- ATPase activity were mainly seen at the cytoplasmic side of the plasma membrane. Few cerium phosphate deposits also were observed on the nuclear envelope. In both species, after 1 or 3 h 2°C chilling, antimonate Ca2+ deposits increased in the cytosol and the nucleus, but cerium phosphate deposits showed no visible difference compared to their corresponding 25°C seedlings. After 12, 24, or 72 h chilling, maize seedlings still maintained a high level of antimonate Ca2+ deposits in the cytosol and the nucleus. During these periods, maize Ca2+-ATPase, as indicated by the number of cerium phosphate deposits, became less and less active as chilling proceeded. In winter wheat, the increased cytosolic and nuclear antimonate Ca2+ deposits were restored to a low resting level after 12, 24, or 72 h chilling, while the Ca2+-ATPase was maintained active, contrary to maize scenario. The transient cytosolic and nuclear Ca2+ increase and the activities of Ca2+-ATPase during chilling are discussed in relation to plant chilling injury and cold acclimation.
ACCESSION #
79307432

 

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics