Klimas, Ramutis; Klimiene, Asta; Pileckas, Vidmantas
June 2012
Veterinarija ir Zootechnika;2012, Vol. 58 Issue 80, p20
Academic Journal
The aim of this study was to determine the effects of the epididymis sample storage conditions and season on the sperm morphological characteristics of wild boars (Sus scrofa L.). The samples were collected from males hunted in northern Lithuania. Straight after shooting the animal and cutting out the both testis, the epididymis was dissected and divided into three parts. One part was placed on a freezing element (4±2°C, the second one into a 3.0 % sodium citrate and the third one into a preservative solution. For determination of seasonal effects, the samples were placed only into a preservative solution. In the laboratory, all the epididymis were broken up and flushed spermatozoa were dyed with eosin. Morphological characteristics of sperm were evaluated by the accepted methodology. Sample storage conditions in 20-24 hours period had no effect on the sperm morphological characteristics of wild boar males (n=6). The study of the seasonal effects on the sperm morphological properties of European wild boar indicated that during the breeding season (November and December) the number of intact spermatozoa in the semen of second year males (n=5) and 3 year or older males (n=6) was similar and accounted for 54.0 and 52.0 % respectively. During the off-season (May and June), the average number of intact spermatozoa in the semen of second year males (n=5) and 3 year or older males (n=6) was respectively 16.2 and 13.0 % or by 37.8 and 39.0 % lower (P<0.001) than that in the semen of contemporary wild boar males hunted at the time of oestrus. In the summer time, the second-year wild boar males can inseminate the second year gilts in heat of the same group what leads to degeneration due to close inbreeding. Older wild boar males should be preserved in order to preserve the reproductive traits of wild boar groups and their genetic values. The seasonal changes of the sexual cycle might have been influenced by global warming and good nutrition conditions throughout the year.


Related Articles

  • Immunohistochemical study of galectin-3 in mature and immature bull testis and epididymis. Hwanglyong Kim; Meejung Ahn; Changjong Moon; Seungjoon Kim; Youngheun Jee; Hong-Gu Joo; Taekyun Shin // Journal of Veterinary Science;2008, Vol. 9 Issue 4, p339 

    Galectin-3, a member of the β-galactoside-binding protein family, has been implicated in mammalian sperm maturation. We examined galectin-3 expression in the testis and epididymis of sexually mature and immature bulls. Western blot analysis showed varying levels of galectin-3 in the bull...

  • Epididymal Sperm Granuloma Induced by Chronic Administration of 2-Methylimidazole in B6C3F 1 Mice. Tani, Yoshiro; Foster, Paul M.; Sills, Robert C.; Chan, Po C.; Peddada, Shyamal D.; Nyska, Abraham // Toxicologic Pathology;2005, Vol. 33 Issue 3, p313 

    Two-year mouse and rat bioassay studies of 2-methylimidazole (2-MI) conducted by the National Toxicology Program revealed that epididymal sperm granuloma(SG)s occurred only in male B6C3F 1 mice in a dose-related manner. The present study characterized 2-MI-induced SGs in these epididymides....

  • New Emerging Androgenic Actions in the Regulation of Sperm Production and Function. Correia, S.; Socorro, S. // Theriogenology Insight;Apr2014, Vol. 4 Issue 1, p33 

    Androgenic actions are determinant for sperm production and function and, thus, for male fertility. Androgens exert their effects by interaction with the androgen receptor (AR), a transcription factor that modulates gene expression in target-cells and tissues. Variants of AR protein have been...

  • In-depth mapping of human testicular and epididymal proteins and their functional association with spermatozoa. XUEXIA LIU; FUJUN LIU // Molecular Medicine Reports;2015, Vol. 12 Issue 1, p173 

    The mammalian testis and epididymis are responsible for spermatozoa production and maturation, which contributes to male fertility. Predominantly expressed proteins in the testis and epididymis were suggested to be involved in the key functions or pathways in spermatogenesis and sperm...

  • Effect of piperine on the epididymis of adult male rats. D'Cruz, S. C.; Mathur, P. P. // Asian Journal of Andrology;Dec2005, Vol. 7 Issue 4, p363 

    Aim: To study the effect of piperine on the epididymal antioxidant system of adult male rats. Methods: Adult male rats were orally administered piperine at doses of 1 mg/kg, 10 mg/kg and 100 mg/kg body weight each day for 30 consecutive days. Twenty-four hours after the last treatment, the rats...

  • Evolution of sperm morphology in anurans: insights into the roles of mating system and spawning location. Yu Zeng; Shang Ling Lou; Wen Bo Liao; Jehle, Robert // BMC Evolutionary Biology;2014, Vol. 14 Issue 1, p1 

    Background The degree of postcopulatory sexual selection, comprising variable degrees of sperm competition and cryptic female choice, is an important evolutionary force to influence sperm form and function. Here we investigated the effects of mating system and spawning location on the evolution...

  • Genotype-Independent Transmission of Transgenic Fluorophore Protein by Boar Spermatozoa. Garrels, Wiebke; Holler, Stephanie; Taylor, Ulrike; Herrmann, Doris; Struckmann, Christina; Klein, Sabine; Barg-Kues, Brigitte; Nowak-Imialek, Monika; Ehling, Christine; Rath, Detlef; Ivics, Zoltán; Niemann, Heiner; Kues, Wilfried A. // PLoS ONE;2011, Vol. 6 Issue 11, p1 

    Recently, we generated transposon-transgenic boars (Sus scrofa), which carry three monomeric copies of a fluorophore marker gene. Amazingly, a ubiquitous fluorophore expression in somatic, as well as in germ cells was found. Here, we characterized the prominent fluorophore load in mature...

  • Study on the relationship between oligoasthenospermia and accessory genital glands markers. Deng, Shun-Mei; Wen, Ren-Qian; Huang, Jian-Chu; Liu, Mei-Yi; Tang, Li-Xing; Jiang, Yan-Jia; Chen, Ai-Ping // Asian Journal of Andrology;Mar2005, Vol. 7 Issue 1, p70 

    To verify the effect of the accessory genital gland on male fertility.The relevant seminal parameters of 32 normal donors and 35 patients with oligoasthenospermia were measured by analyses of semen routine, sperm morphology and accessory genital glands markers.The percentage of normal...

  • Comprehensive assessment of the effect of Sphenocentrum jollyanum root extract on male reproductive activity in albino rats. RAJI, YINUSA; FADARE, OLAYEMI O.; ADISA, RAHMAT A.; SALAMI, SHAKIRU A. // Reproductive Medicine & Biology;Dec2006, Vol. 5 Issue 4, p283 

    Aim: To evaluate the effect of methanol extract from the Sphenocentrum jollyanum root on male reproductive activity. Methods: Male albino rats were treated orally with distilled water (vehicle for the extract; control) and 50, 100 and 150 mg kg−1 body weight of Sphenocentrum jollyanum...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics