TITLE

FTIR spectroscopic study of molecular organization of the antibiotic amphotericin B in aqueous solution and in DPPC lipid monolayers containing the sterols cholesterol and ergosterol

AUTHOR(S)
Gagoś, Mariusz; Arczewska, Marta
PUB. DATE
August 2012
SOURCE
European Biophysics Journal;Aug2012, Vol. 41 Issue 8, p663
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Langmuir monolayers of amphotericin B (AmB) were investigated by recording π-A isotherms under different pH conditions. To gain a better insight into antibiotic-membrane interactions they were monitored by use of the ATR-FTIR spectroscopy. It was observed for AmB monolayers that the limiting molecular area was larger at high than at neutral pH. Analysis of FTIR spectra at different pH revealed substantial differences, depending on ionic state, for different orientations of AmB molecules. These results enable better understanding of the participation of functional groups in the interactions between AmB and sterol-containing DPPC membranes. AmB molecules incorporated into two-component lipid monolayers bind strongly to the ergosterol-rich membrane (maximum penetration surface pressures ca 35 mN/m). The FTIR spectra revealed that the ionic state of AmB and the presence of sterols led to changes in membrane fluidity and molecular packing of the AmB molecules in the lipid membranes. These investigations should be further investigated to discover the molecular mechanism responsible for the mode of action AmB in biological systems.
ACCESSION #
79194580

 

Related Articles

  • Effect of Aggregation of Amphotericin B on Lysophosphatidylcholine Micelles as Related to Its Complex Formation with Cholesterol or Ergosterol1. Kawabata, Manami; Onda, Maki; Mita, Tomoyoshi // Journal of Biochemistry;2001, Vol. 129 Issue 5, p725 

    The effect of aggregation of amphotericin B (AmB), as well as the complex formation of AmB with cholesterol or ergosterol, was investigated in micelles and vesicles. AmB in lysophosphatidylcholine (LPC) micelles adopted a more favorable monomeric form than that in other drug formulations. At an...

  • Synthesis, Spectroscopic and Theoretical Studies of New Dimeric Quaternary Alkylammonium Conjugates of Sterols. Brycki, Bogumił; Koenig, Hanna; Kowalczyk, Iwona; Pospieszny, Tomasz // Molecules;Jul2014, Vol. 19 Issue 7, p9419 

    New dimeric quaternary alkylammonium conjugates of sterols were obtained by two step reactions of ergosterol, cholesterol and cholestanol with bromoacetic acid bromide, followed by bimolecular nucleophilic substitution with N,N,N',N'-tetramethyl- 1,3-propanediamine,...

  • The adsorptive removal of a cationic drug from aqueous solution using poly (methacrylic acid) hydrogels. Bajpai, S. K.; Chand, Navin; Mahendra, Manika // Water SA;Jan2014, Vol. 40 Issue 1, p49 

    This study describes adsorptive removal of the antibiotic drug ciprofloxacin hydrochloride from simulated water using poly(methacrylic acid) (PMAA) as adsorbent. The adsorbent was characterised by various instrumental techniques such as X-ray diffraction (XRD), differential scanning calorimetry...

  • Development of modified porous starch as a carrier to improve aqueous solubility. Deveswaran, R.; Sravya, Maddukuri; Bharath, S.; Basavaraj, B. V.; Madhavan, V. // Advances in Applied Science Research;Feb2012, Vol. 3 Issue 1, p162 

    The objective of this work was to improve aqueous solubility of poorly water soluble drugs by a modified porous starch as solid dispersion carrier. The yield of the porous starch was found to be 80%. The flow property of the prepared porous starch was found to be good, with good compressibility...

  • Adsorption Behavior of Acid Bordeaux B from Aqueous Solution onto Waste Biomass of Enteromorpha prolifera. Yongfu Li; Fanping Meng; You Zhou // Polish Journal of Environmental Studies;2014, Vol. 23 Issue 3, p783 

    Enteromorpha prolifera green algae is the main species that causes green tide in China's Yellow Sea. To effectively realize the resourceful utilization of this biomass, batch experiments were carried out to investigate factors that impact the Acid Bordeaux B (ABB) absorption of E. prolifera...

  • Probing Amphotericin B Single Channel Activity by Membrane Dipole Modifiers. Ostroumova, Olga S.; Efimova, Svetlana S.; Schagina, Ludmila V. // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    The effects of dipole modifiers and their structural analogs on the single channel activity of amphotericin B in sterolcontaining planar phosphocholine membranes are studied. It is shown that the addition of phloretin in solutions bathing membranes containing cholesterol or ergosterol decreases...

  • Determination Residues of Penicillin G and Cloxacillin in Raw Cow Milk Using Fourier Transform Near Infrared Spectroscopy. Dračková, Michaela; Navrátilová, Pavlína; Hadra, Luboš; Vorlová, Lenka; Hudcová, Lenka // Acta Veterinaria Brno;Dec2009, Vol. 78 Issue 4, p685 

    The objective of this study was to study the use of Fourier transform near infrared spectroscopy (FTNIR) combined with the partial least square (PLS) method for determining the residues of penicillin and cloxacillin in raw milk. The spectra were measured in the reflectance mode with...

  • Synthesis of Cholesterol-Conjugated Magnetic Nanoparticles for Purification of Human Paraoxonase 1. Samra, Zahoor Qadir; Shabir, Sadaf; Rehmat, Zainab; Zaman, Mariam; Nazir, Aqsa; Dar, Nadia; Athar, Muhammad Amin // Applied Biochemistry & Biotechnology;Oct2010, Vol. 162 Issue 3, p671 

    Human serum paraoxonase 1 (PON1) is known as an antioxidant and is also involved in the detoxification of many compounds. In this study, a novel purification strategy was employed to purify the PON1 by using cholesterol-conjugated magnetic nanoparticles. Magnetic nanoparticles were synthesized...

  • Potential toxicity of amphenicol antibiotic: binding of chloramphenicol to human serum albumin. Chen, Huilun; Rao, Honghao; He, Pengzhen; Qiao, Yongxiang; Wang, Fei; Liu, Haijun; Cai, Minmin; Yao, Jun // Environmental Science & Pollution Research;Oct2014, Vol. 21 Issue 19, p11340 

    Antibiotics are widely used in daily life but their abuse has posed a potential threat to human health. To evaluate the toxicity of chloramphenicol (CAP) at the protein level, the interaction between CAP and human serum albumin (HSA) was investigated by fluorescence, Ultraviolet-visible (UV-Vis)...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics