Molecular identification of Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) larvae collected from dogs (Canis familiaris) in Jos South, Plateau State, Nigeria

Ogo, Ndudim I.; Onovoh, Emmanuel; Okubanjo, Oluyinka O.; Galindo, Ruth C.; de la Lastra, Jose-Manuel P.; de la Fuente, Jose
March 2012
Onderstepoort Journal of Veterinary Research;2012, Vol. 79 Issue 1, Special section p1
Academic Journal
Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) after the initial morphological identification. This is the first molecular identification of any myiasis-causing fly species in Nigeria and may serve as a reliable alternative to morphological identification where samples are not well preserved or difficult to identify to species level.


Related Articles

  • The Intestinal Bacterial Community in the Food Waste-Reducing Larvae of Hermetia illucens. Hyunbum Jeon; Soyoung Park; Jiyoung Choi; Gilsang Jeong; Sang-Beom Lee; Youngcheol Choi; Sung-Jae Lee // Current Microbiology;May2011, Vol. 62 Issue 5, p1390 

    it is known that food waste can be reduced by the larvae of Hermetia illucens (Black soldier fly, BSF), the scientific and commercial value of BSF larvae has increased recently. We hypothesised that the ability of catabolic degradation by BSF larvae might be due to intestinal microorganisms....

  • Probe generation by PCR coupled with ligation. Ilyin, Sergey E.; Plata-Salamán, Carlos R. // Nature Biotechnology;Jun99, Vol. 17 Issue 6, p608 

    An approach for the efficient production of RNA probes.

  • Molecular Discrimination of Garfish Hyporhamphus (Beloniformes) Larvae in Southern Australian Waters. Noell, Craig J.; Donnellan, S.; Foster, R.; Haigh, L. // Marine Biotechnology;Nov2001, Vol. 3 Issue 6, p509 

    A multiplex polymerase chain reaction (PCR) assay developed for discrimination between garfish larvae (family Hemiramphidae, order Beloniformes) found in southern Australian waters was based on speciesspecific amplification of part of the mitochondrial control region. The species were easily...

  • Occurrence and molecular identification of Anisakis spp. from the North African coasts of Mediterranean Sea. Sarra Farjallah; Badreddine Slimane; Marina Busi; Lia Paggi; Nabil Amor; Hager Blel; Khaled Said; Stefano D’Amelio // Parasitology Research;Feb2008, Vol. 102 Issue 3, p371 

    Abstract  Larval forms of the genus Anisakis were reported infecting several fish species from the North African coasts of central Mediterranean Sea. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis was used to investigate the...

  • Feeding selectivity of bivalve larvae on natural plankton assemblages in the Western English Channel. Lindeque, Penelope; Dimond, Anna; Harmer, Rachel; Parry, Helen; Pemberton, Katharine; Fileman, Elaine // Marine Biology;Feb2015, Vol. 162 Issue 2, p291 

    Meroplankton, including bivalve larvae, are an important and yet understudied component of coastal marine food webs. Understanding the baseline of meroplankton ecology is imperative to establish and predict their sensitivity to local and global marine stressors. Over an annual cycle (October...

  • Vertical transmission of Bartonella schoenbuchensis in Lipoptena cervi. de Bruin, Arnout; van Leeuwen, Arieke Docters; Jahfari, Setareh; Takken, Willem; Földvári, Mihály; Dremmel, László; Sprong, Hein; Földvári, Gábor // Parasites & Vectors;2015, Vol. 8 Issue 1, p1 

    Background: Lipoptena cervi (Diptera: Hippoboscidae) is a hematophagous ectoparasite of cervids, which is considered to transmit pathogens between animals and occasionally to humans. The principal life stage that is able to parasitize new hosts is a winged ked that just emerged from a pupa....

  • Isolation and preservation of schistosome eggs and larvae in RNAlater® facilitates genetic profiling of individuals.  // Parasites & Vectors;2009, Vol. 2 Issue 1, p50 

    The article presents a report on some technical and logistical challenges preventing epidemiological studies on isolation and preservation of schistosome eggs and larvae in RNAlater. An alternative methodology, which enables better preservation of large numbers of individual schistosome larval...

  • Positional cloning of a gene responsible for the cts mutation of the silkworm, Bombyx mori. Ito, Katsuhiko; Kidokoro, Kurako; Katsuma, Susumu; Shimada, Toru; Yamamoto, Kimiko; Mita, Kazuei; Kadono-Okuda, Keiko; Bell, J. // Genome;Jul2012, Vol. 55 Issue 7, p493 

    The larval head cuticle and anal plates of the silkworm mutant cheek and tail spot ( cts) have chocolate-colored spots, unlike the entirely white appearance of the wild-type (WT) strain. We report the identification and characterization of the gene responsible for the cts mutation. Positional...

  • Differential expression of microRNA-2b with potential target coding P25 in the fifth instar larvae posterior silk gland of the silkworm. Huang, Yong; Zou, Quan; Shen, Xing; Tang, Shun; Wang, Sheng; Zhao, Qiao // Molecular Biology;Aug2011, Vol. 45 Issue 4, p576 

    MicroRNAs (miRNAs) are a class of non-protein coding small RNAs that regulate a gene expression at the post-transcriptional level. Using in silico screening, we found that the 3′-untranslated regions of the P25 gene mRNA are perfectly complementary to nucleotides 2-8 at the 5′ end of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics