TITLE

THE EFFECT OF DIFFERENT DILUTION RATES ON POST-THAW QUALITY OF RAM SEMEN FROZEN IN TWO DIFFERENT EGG-YOLK FREE EXTENDERS

AUTHOR(S)
Akçay, Ergun; Kulaksiz, Recai; Daşkin, Ali; Çebi, Çiğdem; Tekin, Koray
PUB. DATE
June 2012
SOURCE
Slovenian Veterinary Research;2012, Vol. 49 Issue 2, p97
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The objective of the present studywas to evaluate the effects of pre-freezing sperm concentration (200,400 or 800x106 spermatozoa/ml) using two commercial extenders (Bioexcell® and Andromed®) on post-thaw survival and acrosomal status of ram spermatozoa. Semen samples were obtained from the 5 mature Karayaka rams (aged 2-3 yr) and a total of 30 ejaculates collected from each male twice a week for 3 weeks with the aid of an artificial vagina, during the non-breeding season (February, winter). Semen extended in Bioexcell® or Andromed® diluents, was loaded into 0.25 ml straws and equilibrated at 4°C for 2 h. Straws were frozen in the vapour of liquid nitrogen and then stored at-196°C. Afterthawing (at 37°C for 30 sec), sperm motility, acrosomal status and membrane integritywere assessed. Pre-freezing sperm concentration influenced (P<0.001) frezability of spermatozoa and affected all the in vitro parameters at 400x106 and 800x106 spermatozoa/ml negatively regardless of the extender. Decreasing the sperm concentration into 200x106 spermatozoa/ml influenced positively the percentage of sperm motility and membrane integrity extended in Bioexcell® (40%, 29%) and Andromed®(43%, 32%). The lowest percentage of abnormal acrosomewas also described at lowest sperm concentration (200x106 spermatozoa/ml) in both extenders as 29 and 26%. It was concluded that significant differences exist between the dilution rates orsperm concentrations. Lowersperm concentrations or higherdilution rates with the commercial extenders were better to protect sperm from damages during cryopreservation.
ACCESSION #
78550540

 

Related Articles

  • Effects of Different Levels of Pigeon Egg Yolk in Extenders on the Post-Thaw Semen Quality of Sahiwal Bulls. Jamil-ur-Rahman, Hafez; Ahmad, Nazir; Najib-ur-Rahman; Waheed, Salman; Ahmad, Maqbool; Younis, Muhammad; Ahmad, Tanveer // Pakistan Veterinary Journal;Jul-Sep2012, Vol. 32 Issue 3, p315 

    In this study, effects of replacing chicken egg yolk (CEY) with pigeon egg yolk (PEY) in extenders on post-thaw semen quality in Sahiwal bulls were investigated. Attempts were also made to see if post thaw semen quality was affected by reducing PEY level in the extender. Twenty four semen...

  • Effect of Soybean Based Extenders on Sperm Parameters of Holstein-Friesian Bull During Liquid Storage at 4°C. Rehman, F. U.; Qureshi, M. S.; Khan, R. U. // Pakistan Journal of Zoology;2014, Vol. 46 Issue 1, p185 

    Animal origin egg yolk extender has been associated with microbial contamination and interference with microscopic examination. Therefore, replacement of such extender with plant origin compound is inevitable without compromising the desired qualities of an ideal extender. In the current...

  • INCREASED FERTILIZATION RATES AFTER IN-VITRO CULTURE OF FROZEN-THAWED TESTICULAR IMMOTILE SPERM IN NON-OBSTRUCTIVE AZOOSPERMIC PATIENTS. Nunez-Calonge, Rocio; Gago, Marta; Cortes, Susana; Garcia, Aurea; Lopez, Patricia; Caballero, Pedro // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jun2010 S2 Supplement, Vol. 20, pS52 

    Aim: The aim of this study was to optimise the use of freeze/thaw testicular immotile spermatozoa from nonobstructive azoospermia patients and to analyse the outcome of intracytoplasmic sperm injection (ICSI) of such spermatozoa. Method: Testicular specimens were retrieved and cryopreserved from...

  • Influence of handling and thawing methods on the quality of bovine sperm frozen with egg yolk-based extender and packaged in 0.5-ml straws. Okamura, Riko // Japanese Journal of Veterinary Research;Aug2012, Vol. 60 Issue 2/3, p170 

    The article presents a study which aims to investigtae the influence of the handling and thawing straws on sperm quality. The study assesses the viability, acromosal integrity, motility and active mitochondria in sperm after thawing. Results reveal that to prevent sperm damage, straws should be...

  • Electrolyte-Free Cool Preservation Does Not Negatively Impact Capacitation, AR, or Penetration.  // Fertility Weekly;1/28/2002, p3 

    Examines the penetrating capacity of human spermatozoa cool preserved in electrolyte-free (EF) solution. Survival of human spermatozoa in a EF solution; Capability of the method to retain the motility, viability and function of sperms; Indication of sperm's normal penetrating capacity.

  • Effects on Postoperative Motility and DFI. Baker, Karen // Fertility Weekly;4/21/2014, p5 

    The article reports on the findings of a research which aims to determine preoperative variables that are predictive of improvement in postoperative semen values and variables that predict pregnancy after varicocelectomy. According to researchers, several semen parameters are significantly...

  • Seminal malondialdehyde levels and oxidative stress in obese male infertility. Najafi, Mohsen; Sreenivasa, G.; Aarabi, Mahmoud; Dhar, Murali; Babu, M. Suresh; Malini, Suttur S. // Journal of Pharmacy Research;2012, Vol. 5 Issue 7, p3597 

    Objective: To evaluate Malondialdehyde (MDA) levels in obese and non-obese infertile males in comparison with non obese fertile males and to correlate with sperm motility, function and morphology. Materials and methods: Ninety males, divided in 3 groups including obese infertile (n=30), non...

  • EVALUATION OF SPERM QUALITY AT A NATIONAL LEVEL: LOGISTIC, RECRUITMENT, ANALYTICAL AND STATISTICAL PROBLEMS ENCOUNTERED IN SWITZERLAND. Vargas, Josefina; Parapanov, Roumen; Van Den Bergh, Marc; Stettler, Eric; Crettaz, Pierre; Senn, Alfred; Germond, Marc // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jun2010 S2 Supplement, Vol. 20, pS57 

    Aim: For decades, numerous studies have reported a decline in sperm quality among industrialised countries. Explanations for this decline point toward environmental factors acting on animals and humans. More recently, the detrimental role of endocrine disruptors during foetal development of the...

  • Effect of different egg yolk levels on post thaw quality of Kundhi buffalo bull semen. Fatih, A.; Samo, M. U.; Marghazani, I. B.; Nasrullah, M. A. Kakar; Nawaz, A. // Revista Veterinaria;2010, Vol. 21 Issue 1, p864 

    The study was conducted to evaluate the effect of different levels of egg yolk (20, 15 and 10%) in diluents on post-thaw quality of Kundhi buffalo bull semen. It was observed that all the ejaculates were creamy white in color. The mean volume, pH, mass activity, sperm concentration and motility...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics