TITLE

THE EFFECT OF DIFFERENT DILUTION RATES ON POST-THAW QUALITY OF RAM SEMEN FROZEN IN TWO DIFFERENT EGG-YOLK FREE EXTENDERS

AUTHOR(S)
Akçay, Ergun; Kulaksiz, Recai; Daşkin, Ali; Çebi, Çiğdem; Tekin, Koray
PUB. DATE
June 2012
SOURCE
Slovenian Veterinary Research;2012, Vol. 49 Issue 2, p97
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The objective of the present studywas to evaluate the effects of pre-freezing sperm concentration (200,400 or 800x106 spermatozoa/ml) using two commercial extenders (Bioexcell® and Andromed®) on post-thaw survival and acrosomal status of ram spermatozoa. Semen samples were obtained from the 5 mature Karayaka rams (aged 2-3 yr) and a total of 30 ejaculates collected from each male twice a week for 3 weeks with the aid of an artificial vagina, during the non-breeding season (February, winter). Semen extended in Bioexcell® or Andromed® diluents, was loaded into 0.25 ml straws and equilibrated at 4°C for 2 h. Straws were frozen in the vapour of liquid nitrogen and then stored at-196°C. Afterthawing (at 37°C for 30 sec), sperm motility, acrosomal status and membrane integritywere assessed. Pre-freezing sperm concentration influenced (P<0.001) frezability of spermatozoa and affected all the in vitro parameters at 400x106 and 800x106 spermatozoa/ml negatively regardless of the extender. Decreasing the sperm concentration into 200x106 spermatozoa/ml influenced positively the percentage of sperm motility and membrane integrity extended in Bioexcell® (40%, 29%) and Andromed®(43%, 32%). The lowest percentage of abnormal acrosomewas also described at lowest sperm concentration (200x106 spermatozoa/ml) in both extenders as 29 and 26%. It was concluded that significant differences exist between the dilution rates orsperm concentrations. Lowersperm concentrations or higherdilution rates with the commercial extenders were better to protect sperm from damages during cryopreservation.
ACCESSION #
78550540

 

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