TITLE

Direct sequencing of Arabidopsis thaliana RNA reveals patterns of cleavage and polyadenylation

AUTHOR(S)
Sherstnev, Alexander; Duc, Céline; Cole, Christian; Zacharaki, Vasiliki; Hornyik, Csaba; Ozsolak, Fatih; Milos, Patrice M; Barton, Geoffrey J; Simpson, Gordon G
PUB. DATE
July 2012
SOURCE
Nature Structural & Molecular Biology;Jul2012, Vol. 19 Issue 8, p845
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
It has recently been shown that RNA 3′-end formation plays a more widespread role in controlling gene expression than previously thought. To examine the impact of regulated 3′-end formation genome-wide, we applied direct RNA sequencing to A. thaliana. Here we show the authentic transcriptome in unprecedented detail and describe the effects of 3′-end formation on genome organization. We reveal extreme heterogeneity in RNA 3′ ends, discover previously unrecognized noncoding RNAs and propose widespread reannotation of the genome. We explain the origin of most poly(A)+ antisense RNAs and identify cis elements that control 3′-end formation in different registers. These findings are essential to understanding what the genome actually encodes, how it is organized and how regulated 3′-end formation affects these processes.
ACCESSION #
78297978

 

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