TITLE

Antiserum production in immunized camel by the venom of Hemiscorpius lepturus scorpion: evaluation of neutralizing test in vivo

AUTHOR(S)
Behdani, Mahdi; chafi, Mohammad Hosseininejad; Zeinali, Sirous; Karimipour, Morteza; Shahreza, Hossein Khanahmad; Ghasemi, Pooria; Asadzadeh, Nader; Ghamnak, Atieh; Bagheri, Kamran Pooshang; Ahari, Hamed; Shahbazzadeh, Delavar
PUB. DATE
August 2010
SOURCE
Tehran University Medical Journal;Aug2010, Vol. 68 Issue 5, p268
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Scorpion envenomation is considered as one of the Public Health problems in some countries in the world including Iran. Annually, approximately 30,000 scorpion stings happen in Iran from which 12% belongs to Hemiscorpius lepturus (special small closely spaced, bead-shaped jointed tail, similar in the shape to a cows tail, and is locally called "gaodim" (Gao, cow; dim, tail)) with 95% mortality. The main treatment is antiserum therapy which is produced in horse and is the only way to neutralize the venom. Due to the anaphylactic shock of the horse antiserum in some of the stung patients other source of antiserum is recommended. In this study the ability of produced camel antiserum in neutralizing the scorpion venom of Hemiscorpius lepturus was performed in Balb/c model. Methods: Camel is an animal model that genetically is compatible with human genome utilized in this research to produce antiserum against scorpion venom. Two camels were used for immunization with the venom of Hemiscorpius lepturus. ELISA method was used to confirm the immunity. Antiserum was produced and used for neutralizing test. The precipitated antiserum with saturated ammonium sulfate (SAS) was also used to perform the neutralizing test in mice. Results: The results indicated that the amount of 200 μl of antiserum and 400 μl of SAS antiserum were able to neutralize the amount of 1 LD100 of the venom and the survived the mice from death. Conclusion: The result indicated that camel antiserum against scorpion venom is capable to neutralize the crude venom in mice model. Due to the safety of camel serum in human, it is suggested that the produced antiserum in camel can be substitute with the traditional horse antiserum in scorpion stung patients.
ACCESSION #
78154057

 

Related Articles

  • Triage for Leiurus quinquestriatus scorpion envenomation in children — is routine ICU hospitalization necessary? Ben-Abraham, R.; Eshel, G.; Winkler, E.; Weinbroum, A.A.; Barzilay, Z.; Paret, G. // Human & Experimental Toxicology;Dec2000, Vol. 19 Issue 12, p663 

    (1) Leiurus quinquestriatus scorpion (LQS) envenomation is a common public health problem with a similar clinical presentation in the Middle East and worldwide: localized reactions occur in up to 97% of the victims. (2) LQS envenomation in children is potentially fatal since the severity of...

  • Prazosin treatment in the management of scorpion envenomation. Peker, Erdal; Oktar, Suleyman; Dogan, Murat; Kaya, Ergun; Duru, Mehmet // Human & Experimental Toxicology;Mar2010, Vol. 29 Issue 3, p231 

    Scorpion stings represent an important and serious public health problem worldwide due to their high incidence and potentially severe and often fatal clinical manifestations. Children are at greater risk of developing severe cardiac, respiratory, and neurological complications due to lesser body...

  • Hemolytic uremic syndrome following Hemiscorpius lepturus scorpion sting. Valavi, E.; Alemzadeh Ansari, M. J. // Indian Journal of Nephrology;Oct-Dec2008, Vol. 18 Issue 4, p166 

    Scorpion envenomations are a public health problem in many countries. Scorpions are second only to snakes in causing human fatalities from envenomation. Species of scorpions capable of inflicting fatal stings are living in North and South Africa, the Middle East, India, America, Trinidad, and...

  • Scorpion Bite in North West Iran. Shams Vahdati, Samad; Moradi, Narges // Turkish Journal of Emergency Medicine;2012, Vol. 12 Issue 4, p149 

    A letter to the editor related to contamination of bite of the predatory arthropod animal scorpion venom in the topical region of North West Iran is presented.

  • Massasaugas, Michigan, and the American Origins of Snakebite Therapy. BEAUVAIS, THOMAS F.; ADLER, KRAIG // Herpetological Review;2014, Vol. 45 Issue 1, p74 

    The article focuses on the history of origin of Snakebite Therapy by Joseph B. Steere, professor of zoology at the University of Michigan. Topics discussed include experiments to provide immunological foundation on snakebite antiserums, discovery on resistance to venoms, and role of immunologist...

  • Scorpion envenomation in children: an analysis of 99 cases. Uluǧ, Mehmet; Yaman, Yöntem; Yapıcı, Ferda; Can-Uluǧ, Nuray // Turkish Journal of Pediatrics;Mar/Apr2012, Vol. 54 Issue 2, p119 

    Scorpion envenomation is a life-threatening emergency and a common public health problem in many regions of the world, particularly in children. The aim of this retrospective descriptive study was to describe the epidemiological characteristics and some common clinical symptoms and signs,...

  • Clinical and epidemiological aspects of scorpion stings in the northeast region of Brazil. Barros, Rafaella Moreno; Pasquino, Jackeline Araujo; Peixoto, Laisla Rangel; Targino, Isabely Tamarys Gomes; de Sousa, Jorge Alves; de Souza Leite, Renner // Revista Ciência & Saúde Coletiva;apr2014, Vol. 19 Issue 4, p1275 

    Scorpion stings are a serious public health issue in tropical and subtropical countries. This is a descriptive and retrospective study of the clinical-epidemiological characteristics of scorpion sting cases registered in the Health System in the city of Campina Grande, Paraíba state, from...

  • Effect of Tityus serrulatus venom on cytokine production and the activity of murine macrophages. Petricevich, Vera L. // Mediators of Inflammation;Feb2002, Vol. 11 Issue 1, p23 

    The purpose of this study was to investigate the effects of Tityus serrulatus venom (TSV) on murine peritoneal macrophages evaluated in terms of activation. The effects of crude TSV were analysed by detection of cytokines, oxygen intermediate metabolites (H[sub 2]O[sub 2]) and nitric oxide (NO)...

  • Efficacy of scorpion antivenom.  // BMJ: British Medical Journal (Overseas & Retired Doctors Edition;1/8/2011, Vol. 342 Issue 7788, p90 

    An introduction to an article about the effects of monospecific F(ab)2 scorpion antivenom on the cardiac pathophysiological effects of scorpion sting, published within the issue, is presented.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics