Antiserum production in immunized camel by the venom of Hemiscorpius lepturus scorpion: evaluation of neutralizing test in vivo

Behdani, Mahdi; chafi, Mohammad Hosseininejad; Zeinali, Sirous; Karimipour, Morteza; Shahreza, Hossein Khanahmad; Ghasemi, Pooria; Asadzadeh, Nader; Ghamnak, Atieh; Bagheri, Kamran Pooshang; Ahari, Hamed; Shahbazzadeh, Delavar
August 2010
Tehran University Medical Journal;Aug2010, Vol. 68 Issue 5, p268
Academic Journal
Background: Scorpion envenomation is considered as one of the Public Health problems in some countries in the world including Iran. Annually, approximately 30,000 scorpion stings happen in Iran from which 12% belongs to Hemiscorpius lepturus (special small closely spaced, bead-shaped jointed tail, similar in the shape to a cows tail, and is locally called "gaodim" (Gao, cow; dim, tail)) with 95% mortality. The main treatment is antiserum therapy which is produced in horse and is the only way to neutralize the venom. Due to the anaphylactic shock of the horse antiserum in some of the stung patients other source of antiserum is recommended. In this study the ability of produced camel antiserum in neutralizing the scorpion venom of Hemiscorpius lepturus was performed in Balb/c model. Methods: Camel is an animal model that genetically is compatible with human genome utilized in this research to produce antiserum against scorpion venom. Two camels were used for immunization with the venom of Hemiscorpius lepturus. ELISA method was used to confirm the immunity. Antiserum was produced and used for neutralizing test. The precipitated antiserum with saturated ammonium sulfate (SAS) was also used to perform the neutralizing test in mice. Results: The results indicated that the amount of 200 μl of antiserum and 400 μl of SAS antiserum were able to neutralize the amount of 1 LD100 of the venom and the survived the mice from death. Conclusion: The result indicated that camel antiserum against scorpion venom is capable to neutralize the crude venom in mice model. Due to the safety of camel serum in human, it is suggested that the produced antiserum in camel can be substitute with the traditional horse antiserum in scorpion stung patients.


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