TITLE

Differential effects of docosahexaenoic acid on contractions and L-type Ca2+ current in adult cardiac myocytes

AUTHOR(S)
Ferrier, Gregory R.; Redondo, Isabel; Zhu, Jiequan; Murphy, Mary G.
PUB. DATE
June 2002
SOURCE
Cardiovascular Research;Jun2002, Vol. 54 Issue 3, p601
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Beneficial effects of n−3 polyunsaturated fatty acids in Ca2+ overload have been attributed to blockade of L-type Ca2+ current (ICa-L). However, cardiac contractions may be maintained despite block of ICa-L. Objective: This study investigates the cellular basis by which docosahexaenoic acid (DHA), a representative n−3 polyunsaturated fatty acid, inhibits ICa-L while preserving contraction. Methods: Experiments were conducted in adult guinea pig ventricular myocytes with Na+ currents blocked. Contractions initiated by the voltage-sensitive release mechanism (VSRM) and calcium-induced calcium release (CICR) triggered by ICa-L, were activated separately with voltage clamp techniques. Results: DHA (10 μM) inhibited ICa-L and CICR contractions but not VSRM contractions. CICR contractions exhibited a bell-shaped voltage-dependence. However, in the presence of DHA, only contractions with a sigmoidal voltage-dependence characteristic of the VSRM remained. These contractions exhibited inactivation properties characteristic of the VSRM. DHA abolished ICa-L elicited by test steps from −40 mV. Block was voltage-dependent, as residual ICa-L was elicited by steps from −70 mV. Cd2+ inhibited residual current, but not contractions initiated by the same activation steps. Conclusion: Preservation of VSRM contractions during block of ICa-L, may explain the ability of n−3 polyunsaturated fatty acids to inhibit Ca2+ influx while preserving cardiac contractile function.
ACCESSION #
7809520

 

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