TITLE

ATL>Lactoferrin in whole gut lavage fluid as a marker for disease activity in inflammatory bowel disease: comparison with other neutrophil-derived proteins

AUTHOR(S)
Kayazawa, Masanobu; Saitoh, Osamu; Kojima, Keishi; Nakagawa, Ken; Tanaka, Seigou; Tabata, Kazue; Matsuse, Ryoichi; Uchida, Kazuo; Hoshimoto, Masahiro; Hirata, Ichiro; Katsu, Ken-ichi
PUB. DATE
February 2002
SOURCE
American Journal of Gastroenterology;Feb2002, Vol. 97 Issue 2, p360
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
OBJECTIVES:We investigated which neutrophil-derived proteins in whole gut lavage fluid (WGLF) most accurately reflect disease activity in inflammatory bowel disease.METHODS:WGLF was obtained from patients undergoing whole gut lavage as a bowel preparation for colonoscopy. Twenty-seven patients with ulcerative colitis (UC), 23 patients with Crohn’s disease (CD), and 35 control subjects were examined. The concentrations of lactoferrin, polymorphonuclear neutrophil elastase (PMN-E), myeloperoxidase, and lysozyme in WGLF were measured by ELISA. For the assessment of stability, WGLF samples were stored at 37°C for various periods.RESULTS:In UC, the concentrations of lactoferrin, myeloperoxidase, and lysozyme in WGLF had good correlations with colonoscopic grading. Zero, 12, five, and 10 of 28 samples from active UC patients showed normal concentrations of lactoferrin, PMN-E, myeloperoxidase, and lysozyme, respectively. In CD, the concentrations of lactoferrin and myeloperoxidase had good correlations with the Crohn’s disease activity index. Thirteen and seven of 36 samples from inactive CD patients (Crohn’s disease activity index ≤ 150) showed high concentrations of lactoferrin and myeloperoxidase, respectively. Most of them (11/13, 6/7) were found to have ulceration by colonoscopy or small bowel x-ray. The ratio of the lactoferrin concentration in the WGLF supernatant to that in total WGLF was highest among these proteins in all disease groups and control subjects. Lactoferrin and myeloperoxidase showed good stability in WGLF, whereas PMN-E and lysozyme did not.CONCLUSION:Lactoferrin is the most suitable of these proteins for use as a neutrophil-derived WGLF marker of intestinal inflammation.
ACCESSION #
7755151

 

Related Articles

  • Fecal lactoferrin is a sensitive and specific marker in identifying intestinal inflammation. Kane, Sunanda V.; Sandborn, William J.; Rufo, Paul A.; Zholudev, Anna; Boone, James; Lyerly, David; Camilleri, Michael; Hanauer, Stephen B. // American Journal of Gastroenterology;Jun2003, Vol. 98 Issue 6, p1309 

    : ObjectiveLactoferrin is a glycoprotein expressed by activated neutrophils. The aim of this study was to determine the sensitivity and specificity of fecal lactoferrin concentrations for inflammatory bowel disease (IBD) or irritable bowel syndrome (IBS) versus healthy controls.: MethodsFresh...

  • Fecal lactoferrin measurements are useful in the interval assessment of patients with active and inactive inflammatory bowel disease. Walker, Thomas R; Sandborn, William J; Boone, James; Sisco, Daniel; Lyerly, David; Rufo, Paul A // American Journal of Gastroenterology;Sep2003 Supplement, Vol. 98, pS246 

    An abstract of the article concerning measurements of fecal lactoferrin in assessing patients with active and inactive inflammatory bowel disease is presented.

  • Inflammatory bowel disease III.  // Gut;Jul2012 Supplement, Vol. 61, pA389 

    The article presents abstracts on medical topics of Great Britain which include the clinical trials on inflammatory bowel disease, efficacy of the faecal lactoferrin on the inflammatory bowel disease and the results of cytomegalovirus colitis on patients.

  • Fecal Lactoferrin as a Marker for Disease Activity in Inflammatory Bowel Disease: Comparison with Other Neutrophil-derived Proteins. Sugi, Kazunori; Saitoh, Osamu; Hirata, Ichiro; Katsu, Ken-ichi // American Journal of Gastroenterology;May1996, Vol. 91 Issue 5, p927 

    Objectives: 1) To investigate which neutrophil-derived proteins in feces most accurately reflect disease activity in inflammatory, bowel disease. 2) To examine the extracellular release of these proteins by activated neutrophils and their stability in feces by in vitro study. Methods: We studied...

  • Fecal Eosinophil Granule-Derived Proteins Reflect Disease Activity in Inflammatory Bowel Disease. Saitoh, Osamu; Kojima, Keishi; Sugi, Kazunori; Matsuse, Ryoichi; Uchida, Kazuo; Tabata, Kazue; Nakagawa, Ken; Kayazawa, Masanobu; Hirata, Ichiro; Katsu, Ken-ichi // American Journal of Gastroenterology;Dec1999, Vol. 94 Issue 12, p3513 

    OBJECTIVES: The aims of this study were: 1) to examine whether the fecal levels of eosinophil granule-derived proteins reflect disease activity in inflammatory bowel disease (IBD); and 2) to examine the extracellular release of these proteins from eosinophils and their stability in feces by an...

  • Calprotectin and lactoferrin in the assessment of intestinal inflammation and organic disease. d&'Incà, Renata; dal Pont, Elisabetta; di Leo, Vincenza; Ferronato, Antonio; Fries, Walter; Vettorato, Maria Grazia; Martines, Diego; Sturniolo, Giacomo Carlo // International Journal of Colorectal Disease;Apr2007, Vol. 22 Issue 4, p429 

    Calprotectin and lactoferrin are specific neutrophil-derived proteins, which can be measured in the feces because they are released by cells in inflammatory conditions. We evaluated the efficacy of calprotectin and lactoferrin in detecting organic disease as assessed by colonoscopy. The study...

  • Calprotectin and lactoferrin in the assessment of intestinal inflammation and organic disease. d&'Incà, Renata; dal Pont, Elisabetta; di Leo, Vincenza; Ferronato, Antonio; Fries, Walter; Vettorato, Maria Grazia; Martines, Diego; Sturniolo, Giacomo Carlo // International Journal of Colorectal Disease;Apr2007, Vol. 22 Issue 4, p429 

    Calprotectin and lactoferrin are specific neutrophil-derived proteins, which can be measured in the feces because they are released by cells in inflammatory conditions. We evaluated the efficacy of calprotectin and lactoferrin in detecting organic disease as assessed by colonoscopy. The study...

  • Noninvasive Assessment of Intestinal Inflammation: Inflammatory Bowel Disease vs. Irritable Bowel Syndrome. Quig, David W.; Higley, Meghan // Townsend Letter for Doctors & Patients;Jan2006, Issue 270, p74 

    The article discusses the differential diagnosis between inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS). Upon analysis, fecal lactoferrin and fecal lysozyme is detected to be elevated in IBD but not in IBS. Even though IBD and IBS share some common symptoms, IBD is a much...

  • Measuring disease activity in Crohn's disease: what is currently available to the clinician. D'Incà, Renata; Caccaro, Roberta // Clinical & Experimental Gastroenterology;2014, Vol. 7, p151 

    Crohn's disease (CD) is a chronic inflammatory bowel disease characterized by a relapsing-remitting clinical behavior and dominated by intestinal inflammation. Being a chronic disorder that with time develops into a disabling disease, it is important to monitor the severity of inflammation to...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics