TITLE

Actin Mediates Secretory Immunoglobulin A Transport: Effect of Ethanol

AUTHOR(S)
Diebel, Lawrence N.; Liberati, David M.; Saini, Manmit S.; Dulchavsky, Scott A.; Diglio, Clement A.; Brown, William J.
PUB. DATE
September 2002
SOURCE
American Surgeon;Sep2002, Vol. 68 Issue 9, p769
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Secretory immunoglobulin A (IgA) is the principle antibody protecting against pathogens at mucosal sites. Ethanol (EtOH) exposure is related to adverse effects on the enterocyte cytoskeleton. The aim of this study was to assess the role of normal cytoskeletal function on IgA transcytosis and its modulation by EtOH by studying Madin-Darby canine kidney (MDCK) cells transfected with the polyimmunoglobulin receptor. MDCK cells were grown as confluent monolayers and treated with 5 per cent EtOH, cytochalasin D (Cyto-D, a cytoskeletal destabilizer), or pretreatment with prostaglandin E[sub 2] (a cytoskeletal stabilizer) followed by EtOH. Media alone served as control. IgA was then added to the basolateral side of the chambers, and apical samples were taken for enzyme-linked immunosorbent assay analysis at 0, 3, and 12 hours. Dimeric IgA transcytosis increased in all groups and was significantly depressed by 5 per cent EtOH and Cyto-D. Morphological slides revealed aggregation of actin after Cyto-D treatment. Prostaglandin E[sub 2] prevented the decrease in IgA transcytosis seen otherwise with 5 per cent EtOH treatment. We conclude that IgA transcytosis is dependent on actin microfilaments of the cytoskeleton. Decreased IgA transport may lead to mucosal immunodeficiency and infectious complications after EtOH exposure.
ACCESSION #
7752128

 

Related Articles

  • Gastroprotective effect of lupeol on ethanol-induced gastric damage and the underlying mechanism. De S. Lira, Silvéria Regina; Rao, Vietla Satyanarayana; Carvalho, Ana Carla S.; Guedes, Marjorie M.; De Morais, Talita C.; De Souza, Antonia L.; Trevisan, Maria Teresa S.; Lima, Alana F.; Chaves, Mariana H.; Santos, Flávia A. // Inflammopharmacology;Aug2009, Vol. 17 Issue 4, p221 

    The effect of lupeol, a natural pentacyclic triterpene on ethanol-induced gastric damage in mice was evaluated. The gastroprotection was assessed by determination of changes in mean gastric lesion area, quantification of mucosal non-protein sulfhydryls (NP-SH), and characterized using drugs that...

  • Adaptive Cytoprotection: An Endoscopic Study in Man. Foschi, D.; Toti, G. L.; Del Soldato, P.; Ferrante, F.; Galeone, M.; Rovati, V. // American Journal of Gastroenterology;Nov1986, Vol. 81 Issue 11, p1035 

    We studied the protective effect of the mild irritant 20% ethanol against the damage caused by the strong irritant 40% ethanol to the duodenal mucosa of 10 healthy volunteers. At time 0, placebo (1% ethanol) or 20% ethanol (6 ml) was sprayed directly onto the duodenal wall through an endoscope....

  • Active smoking depresses prostaglandin synthesis in human gastric mucosa. Quimby, G. Forest; Bonnice, Canan Avunduk; Burstein, Sumner H.; Eastwood, Gregory L.; Quimby, G F; Bonnice, C A; Burstein, S H; Eastwood, G L // Annals of Internal Medicine;May86, Vol. 104 Issue 5, p616 

    To determine the effect of smoking on gastroduodenal mucosal prostaglandin synthesis, endoscopies were done after an overnight fast on 10 nonsmokers, 12 active smokers who smoked four cigarettes in the hour before endoscopy, and then 11 of the smokers who refrained from smoking for 12 hours....

  • Is Food Allergy a Failure of Secretory IgA, a Gatekeeper that is an Electronic Transistor? Zamm, Alfred V. // Journal of Orthomolecular Medicine;2014, Vol. 29 Issue 2, p86 

    A letter to the editor is presented related to the role of secretory IgAas an electronic transistor controlling selection or rejection of molecules in the absorption process in lumen of gastrointestinal tract and food allergens.

  • Alcohol's role in gastrointestinal tract disorders. Bode, Christiane; Bode, J. Christiane // Alcohol Health & Research World;1997, Vol. 21 Issue 1, p76 

    Discusses the important role being played by gastrointestinal (GI) tract in mediating alcohol's effects on the human body and its health. Overview of the GI tract; Effects of alcohol to the oral cavity and the esophagus; Effects of alcohol on stomach functioning; Effects of chronic alcohol...

  • Expression of proteinase-activated receptor 2 on human primary gastrointestinal myofibroblasts and stimulation of prostaglandin synthesis. Binion, David G.; Seymour, Michelle L.; Compton, Steven J.; Hollenberg, Morley D.; MacNaughton, Wallace K. // Canadian Journal of Physiology & Pharmacology;Jul2005, Vol. 83 Issue 7, p605 

    It is known that subepithelial myofibroblast-derived prostaglandin (PG)E2 can regulate intestinal epithelial cell functions, and that proteinase-activated receptor-2 (PAR2) is abundantly expressed in the gastrointestinal tract. Since PAR2 activation has previously been associated with...

  • Effects of lactulose on the intestinal microflora of periparturient sows and their piglets. Krueger, M.; Schroedl, W.; Isik, W.; Lange, W.; Hagemann, L. // European Journal of Nutrition;2002 Supplement 1, Vol. 41, pi26 

    Summary. The periparturient period of animals (and humans) is very stressful and influenced by the microecosystem of the gastrointestinal tract (GIT). Performance and productivity of animal husbandry depend on the health of animal mothers and their offspring. We investigated the influence of...

  • Imaging and Therapy of Gastrointestinal Cancers with Radiolabeled Antibodies. Goldenberg, David M. // American Journal of Gastroenterology;Oct1991, Vol. 86 Issue 10, p1392 

    Radiolabeled antibodies are gaining in application for the imaging and therapy of cancers. Much progress has been made in the use of these new biological reagents for tumors of the gastrointestinal system, and the current status, problems and prospects are reviewed in this paper. It is apparent...

  • Gut mucosa barrier preservation by orally administered IgA-IgG to patients undergoing bone marrow transplantation: a randomised pilot study. Johansson, J-E; Ekman, T // Bone Marrow Transplantation;7/1/99, Vol. 24 Issue 1, p35 

    Intensive cytotoxic therapy with bone-marrow transplantation (BMT) allows a potential cure for haematological malignancies. Protective strategies to minimise haematological toxicities have been successful and currently toxicity to the gastro-intestinal tract is the major cause of...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics