TITLE

Phytoremediation of 2,4-dichlorophenol using wild type and transgenic tobacco plants

AUTHOR(S)
Talano, Melina; Busso, Débora; Paisio, Cintia; González, Paola; Purro, Silvia; Medina, María; Agostini, Elizabeth
PUB. DATE
July 2012
SOURCE
Environmental Science & Pollution Research;Jul2012, Vol. 19 Issue 6, p2202
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction: Transgenic plant strategies based on peroxidase expression or overexpression would be useful for phenolic compound removal since these enzymes play an important role in phenolic polymerizing reactions. Material and methods: Thus, double transgenic (DT) plants for basic peroxidases were obtained and characterized in order to compare the tolerance and efficiency for 2,4-dichlorophenol (2,4-DCP) removal with WT and simple transgenic plants expressing TPX1 or TPX2 gene. Several DT plants showed the expression of both transgenes and proteins, as well as increased peroxidase activity. Results: DT lines showed higher tolerance to 2,4-DCP at early stage of development since their germination index was higher than that of WT seedlings exposed to 25 mg/L of the pollutant. High 2,4-DCP removal efficiencies were found for WT tobacco plants. TPX1 transgenic plants and DT (line d) reached slightly higher removal efficiencies for 10 mg/L of 2,4-DCP than WT plants, while DT plants (line A) showed the highest removal efficiencies (98%). These plants showed an increase of 21% and 14% in 2,4-DCP removal efficiency for solutions containing 10 and 25 mg/L 2,4-DCP, respectively, compared with WT plants. In addition, an almost complete toxicity reduction of postremoval solutions using WT and DT plants was obtained through AMPHITOX test, which indicates that the 2,4-DCP degradation products would be similar for both plants. Conclusion: These results are relevant in the field of phytoremediation application and, moreover, they highlight the safety of using DT tobacco plants because nontoxic products were formed after an efficient 2,4-DCP removal.
ACCESSION #
77508338

 

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