Detection and characterization of a phytoplasma associated with witches'-broom disease of Salvadora persica in India

Kumar, Sachin; Singh, Vibhuti; Lakhanpaul, Suman
July 2012
Journal of General Plant Pathology;Jul2012, Vol. 78 Issue 4, p294
Academic Journal
Salvadora persica trees with symptoms of witches'-broom, little leaf and severe leaf curling were observed in the Biodiversity Park, North Delhi (India) during February 2010. Using a nested-PCR assay targeting two phytoplasma specific loci (16S ribosomal RNA and the ribosomal protein gene) and sequence analysis of the amplified products, we identified the associated phytopathogen as a phytoplasma of group 16SrI. RFLP-mediated characterization revealed that the phytoplasma belongs to subgroup 16SrI-B. Phylogenetic reconstruction also grouped the phytoplasma within group 16SrI. The study reports a new host of phytoplasma in India and worldwide.


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