HBV C Promoter Sp1 Binding Sequence Functionally Substitutes for the Yeast ARS1 ABF1 Binding Site

Yan, Peijun; Mao, Xicheng; Wang, Lei; Zha, Xiliang; Lu, Changde
October 2002
DNA & Cell Biology;Oct2002, Vol. 21 Issue 10, p737
Academic Journal
Transcriptional factors have been implicated in eukaryotic DNA replication. We have studied the potential function of a viral promoter sequence in DNA replication. The hepatitis B virus (HBV) pregenomic promoter is regulated by two enhancers and cis-elements. The G-C rich region between 1734-1754 nt, which contains two SP1 binding sites, is necessary for transcription origin and HBV replication. We found that the Abf1-binding B3 element in yeast ARS1 can be functionally replaced by the viral Sp1-binding DNA sequence, which activates transcription from the HBV C promoter. Further, yeast RAP1 bound to the viral Sp1 binding sites in vitro. These results suggest that RAP1 binds to the Sp1 binding sites and stimulates yeast DNA replication.


Related Articles

  • p53 binds and represses the HBV enhancer: an adjacent enhancer element can reverse the transcription effect of p53. Ori, Assaf; Zauberman, Arie; Doitsh, Gilad; Paran, Nir; Oren, Moshe; Shaul, Yosef // EMBO Journal;1/15/98, Vol. 17 Issue 2, p544 

    The transcription program of the hepatitis B virus (HBV) genome is regulated by an enhancer element that binds multiple ubiquitous and liver-enriched transcription activators. HBV transcription and replication are repressed in the presence of p53. Here we describe a novel molecular mechanism...

  • Characterization of Transcription from TATA-Less Promoters: Identification of a New Core Promoter Element XCPE2 and Analysis of Factor Requirements. Anish, Ramakrishnan; Hossain, Mohammad B.; Jacobson, Raymond H.; Takada, Shinako // PLoS ONE;2009, Vol. 4 Issue 4, p1 

    Background: More than 80% of mammalian protein-coding genes are driven by TATA-less promoters which often show multiple transcriptional start sites (TSSs). However, little is known about the core promoter DNA sequences or mechanisms of transcriptional initiation for this class of promoters....

  • Treatment of children persistently infected with hepatitis B virus: seroconversion or suppression. Nicola Price; Elizabeth H. Boxall // Journal of Antimicrobial Chemotherapy (JAC);Dec2007, Vol. 60 Issue 6, p1189 

    We have reviewed the current strategies regarding the treatment of persistent hepatitis B virus (HBV) in children and compared these with adult strategies. The options for achieving suppression of viral DNA replication versus hepatitis B e antigen to antibody seroconversion have been evaluated....

  • CRISPR/Cas9 nickase-mediated disruption of hepatitis B virus open reading frame S and X. Karimova, Madina; Beschorner, Niklas; Dammermann, Werner; Chemnitz, Jan; Indenbirken, Daniela; Bockmann, Jan-Hendrik; Grundhoff, Adam; Lüth, Stefan; Buchholz, Frank; Wiesch, Julian Schulze zur; Hauber, Joachim // Scientific Reports;9/4/2015, p13734 

    Current antiviral therapies cannot cure hepatitis B virus (HBV) infection; successful HBV eradication would require inactivation of the viral genome, which primarily persists in host cells as episomal covalently closed circular DNA (cccDNA) and, to a lesser extent, as chromosomally integrated...

  • A Novel Regulator Inhibits HBV Gene Expression. Liou, Jieh-Yuan; Jeng, King-Song; Lin, Ching-Gong; Hu, Cheng-Po; Chang, Chungming // Journal of Biomedical Science;1998, Vol. 5 Issue 5, p343 

    This study investigated the expression of HBV genes. In the investigation, a novel phenomenon was found in that the expression of HBV genes on a cloned HBV DNA was inhibited by cotransfection with plasmids containing HBV surface genes. Furthermore, the production of viral particles was also...

  • Hepatitis B Viral DNA Decline at Loss of HBeAg Is Mainly Explained by Reduced cccDNA Load - Down-Regulated Transcription of PgRNA Has Limited Impact. Malmström, Sebastian; Larsson, Simon B.; Hannoun, Charles; Lindh, Magnus // PLoS ONE;Jul2012, Vol. 7 Issue 7, p1 

    Background: Quantification of hepatitis B virus (HBV) DNA and surface antigen (HBsAg) serum levels have become increasingly important for the assessment of clinical stage and response to treatment for chronic hepatitis B. Effective immune clearance results in reduction of viremia by 4-5 log...

  • Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon α. Yu Zhijian; Huang Zhen; Zhang Fan; Yang Jin; Deng Qiwen; Zeng Zhongming // Virology Journal;2010, Vol. 7, p278 

    It has been reported that hepatitis B virus (HBV) core protein (HBc) can inhibit the transcription of human interferon-induced MxA gene. In this study, we investigated whether HBc protein mutations at hot spots (L60V, S87G and I97L) could still inhibit MxA transcription and the potential...

  • Establishment of transcriptional competence in early and late S phase. Zhang, Jianmin; Xu, Feng; Hashimshony, Tamar; Keshet, Ilana; Cedar, Howard // Nature;11/14/2002, Vol. 420 Issue 6912, p198 

    In animal cells, the process of DNA replication takes place in a programmed manner, with each gene region designated to replicate at a fixed time slot in S phase. Housekeeping genes undergo replication in the first half of S phase in all cell types, whereas the replication of many tissue...

  • Hepatitis B Viral X Protein Overcomes Inhibition of E2F1 Activity by pRb on the Human Rb Gene Promoter. Choi, Byung Hyune; Choi, Murim; Jeon, Hyun Yong; Rho, Hyune Mo // DNA & Cell Biology;Feb2001, Vol. 20 Issue 2, p75 

    Hepatitis B virus X (HBx) protein is known as an oncogenic transactivator, E2F1 as a positive regulator of the cell cycle, and pRb as a tumor suppressor. Here, we investigated the functional interactions of these proteins on the human Rb promoter. Interestingly, HBx transactivated the Rb...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics