TITLE

Protecting effect of phosphorylation on oxidative damage of D1 protein by down-regulating the production of superoxide anion in photosystem II membranes under high light

AUTHOR(S)
Chen, Liangbing; Jia, Hongying; Tian, Qiu; Du, Libo; Gao, Yanli; Miao, Xiaoxiang; Liu, Yang
PUB. DATE
June 2012
SOURCE
Photosynthesis Research;Jun2012, Vol. 112 Issue 2, p141
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The physiological significance of photosystem II (PSII) core protein phosphorylation has been suggested to facilitate the migration of oxidative damaged D1 and D2 proteins, but meanwhile the phosphorylation seems to be associated with the suppression of reactive oxygen species (ROS) production, and it also relates to the degradation of PSII reaction center proteins. To more clearly elucidate the possible protecting effect of the phosphorylation on oxidative damage of D1 protein, the degradation of oxidized D1 protein and the production of superoxide anion in the non-phosphorylated and phosphorylated PSII membranes were comparatively detected using the Western blotting and electron spin resonance spin-trapping technique, respectively. Obviously, all of three ROS components, including superoxide anion, hydrogen peroxide and hydroxyl radical are responsible for the degradation of oxidized D1 protein, and the protection of the D1 protein degradation by phosphorylation is accompanied by the inhibition of superoxide anion production. Furthermore, the inhibiting effect of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), a competitor to Q, on superoxide anion production and its protecting effect on D1 protein degradation are even more obvious than those of phosphorylation. Both DCMU effects are independent of whether PSII membranes are phosphorylated or not, which reasonably implies that the herbicide DCMU and D1 protein phosphorylation probably share the same target site in D1 protein of PSII. So, altogether it can be concluded that the phosphorylation of D1 protein reduces the oxidative damage of D1 protein by decreasing the production of superoxide anion in PSII membranes under high light.
ACCESSION #
76351047

 

Related Articles

  • Preventing oxidative stress: a new role for XBP1. Liu, Y.; Adachi, M.; Zhao, S.; Hareyama, M.; Koong, A. C.; Luo, Dan; Rando, T. A.; Imai, K.; Shinomura, Y. // Cell Death & Differentiation;Jun2009, Vol. 16 Issue 6, p847 

    Antioxidant molecules reduce oxidative stress and protect cells from reactive oxygen species (ROS)-mediated cellular damage and probably the development of cancer. We have investigated the contribution of X-box-binding protein (XBP1), a major endoplasmic reticulum stress-linked transcriptional...

  • HIV-1 Tat C phosphorylates VE-cadherin complex and increases human brain microvascular endothelial cell permeability. Mishra, Ritu; Singh, Sunit Kumar // BMC Neuroscience;2014, Vol. 15 Issue 1, p1 

    Background Human brain microvascular endothelial cells (hBMVECs) are integral part of the blood brain barrier. Post-translational modifications of adherens junction proteins regulate the permeability of human brain microvascular endothelial cells. Pro-inflammatory signals can induce tyrosine...

  • Negative feedback regulation of microbe-associated molecular pattern-induced cytosolic Ca transients by protein phosphorylation. Kurusu, Takamitsu; Hamada, Haruyasu; Sugiyama, Yoshimi; Yagala, Toshikazu; Kadota, Yasuhiro; Furuichi, Takuya; Hayashi, Teruyuki; Umemura, Kenji; Komatsu, Setsuko; Miyao, Akio; Hirochika, Hirohiko; Kuchitsu, Kazuyuki // Journal of Plant Research;May2011, Vol. 124 Issue 3, p415 

    Microbe/pathogen-associated molecular patterns (MAMPs/PAMPs) often induce rises in cytosolic free Ca concentration ([Ca]) and protein phosphorylation. Though they are postulated to play pivotal roles in plant innate immunity, their molecular links and the regulatory mechanisms remain largely...

  • Effects of Oviductal Epithelial cell Membrane Proteins on Sperm Functions.  // Fertility Weekly;6/17/2013, p6 

    The article presents a study which discusses the effects of oviductal epithelial cell membrane proteins on human spermatozoa. The study uses the statistical analysis and western blotting to validate the purities of extracted protein fractions. It notes that oviductal epithelial cell membrane...

  • Doing the unexpected: proteins involved in hydrogen peroxide perception. Hancock, John; Desikan, Radhika; Harrison, Judith; Bright, Jo; Hooley, Richard; Neill, Steven // Journal of Experimental Botany;May2006, Vol. 57 Issue 8, p1711 

    A look back at the early literature on reactive oxygen species (ROS) gives the impression that these small inorganic molecules had a singular defined role, that of host defence in mammalian systems. However, it is now known that their roles also include a major part in cell signalling, in a...

  • Increase in Reactive Oxygen Species and Activation of Akt Signaling Pathway in Neuropathic Pain. Renata Guedes; Alex Araújo; Daiane Janner; Adriane Belló-Klein; Maria Ribeiro; Wania Partata // Cellular & Molecular Neurobiology;Dec2008, Vol. 28 Issue 8, p1049 

    Abstract  Neuropathic pain occurs as a result of peripheral or central nervous system injury. Its pathophysiology involves mainly a central sensitization mechanism that may be correlated to many molecules acting in regions involved in pain processing, such as the spinal cord. It has been...

  • ROS-induced ZNF580 expression: a key role for HO/NF-κB signaling pathway in vascular endothelial inflammation. DangLi, Ren; HeKong, Wang; JiQin, Liu; MingHua, Zhang; WenCheng, Zhang // Molecular & Cellular Biochemistry;Jan2012, Vol. 359 Issue 1/2, p183 

    ZNF580, a newly found C2H2 zinc finger transcription factor, was first described by Zhang (GenBank ID: AF184939). Emerging evidence has suggested that reactive oxygen species (ROS) play an important role in redox-sensitive signal transduction, and the vascular endothelium plays a critical role...

  • Modulation of Na+/K+ ATPase Activity by Hydrogen Peroxide Generated through Heme in L. amazonensis. Rocco-Machado, Nathália; Cosentino-Gomes, Daniela; Meyer-Fernandes, José Roberto // PLoS ONE;Jun2015, Vol. 10 Issue 6, p1 

    Leishmania amazonensis is a protozoan parasite that occurs in many areas of Brazil and causes skin lesions. Using this parasite, our group showed the activation of Na+/K+ ATPase through a signaling cascade that involves the presence of heme and protein kinase C (PKC) activity. Heme is an...

  • ANTI-OXIDATIVE EFFECTS AGAINST TIGHT JUNCTION BARRIER DISRUPTION OF PROTEINS ISOLATED FROM CAJANUS CAJAN. Muangman, T.; Klungsupya, P.; Leelamanit, W.; Watari, A.; Yagi, K. // Thai Journal of Pharmaceutical Sciences;SUP2012, Vol. 36, p101 

    The article presents information on a study conducted to investigate the antioxidative effects of proteins isolated from Cajanus cajan, or pigeon pea, against hydrogen peroxide (H2O2)-induced oxidative stress in the intestinal epithelial cells (Caco-2 cells) of human beings. In the study, the...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics