Characterization of the high-affinity uptake of fructose-1,6-bisphosphate by cardiac myocytes

Wheeler, Thomas; Chien, Sufan
July 2012
Molecular & Cellular Biochemistry;Jul2012, Vol. 366 Issue 1/2, p31
Academic Journal
Previously, we reported that fructose-1,6-bisphosphate (FBP) was taken up by rat cardiac myocytes by two processes: a component that was saturable at micromolar levels and a nonsaturable component that dominated at millimolar levels. Here, we continued to characterize the saturable high-affinity component, with the aim of identifying the physiological substrate and role for this activity. ATP, ADP, and AMP inhibited the uptake of FBP with apparent affinities of 0.2-0.5 mM. Fumarate and succinate were very weak inhibitors. Several phosphorylated sugars (ribulose-1,5-phosphate, fructose-1-phosphate, ribose-5-phosphate, and inositol-2-phosphate) inhibited FBP uptake with apparent affinities of 40-500 μM. As in our previous study, no tested compound appeared to bind as well as FBP. The data suggest that the best ligands have two phosphoryl groups separated by at least 8 Å. The rates of FBP uptake were measured from 3° to 37°. The calculated activation energy was 15-50 kJ/mol, similar to other membrane transport processes. Uptake of FBP was tested in several types of cells other than cardiac myocytes, and compared to the uptake of 2-deoxyglucose and l-glucose. While FBP uptake in excess of that of l-glucose was observed in some cells, in no case was the uptake as high as in cardiac myocytes. The physiological substrate and role for the high-affinity FBP uptake activity remain unknown.


Related Articles

  • Q&A: 'Toxic' effects of sugar: should we be afraid of fructose?  // BMC Biology;2012, Vol. 10 Issue 1, p42 

    The article offers information on the role of fructose in human metabolism. It explains whether fructose consumption is a possible contributor for the rise in metabolic diseases and describes fructose metabolism and its impact on liver cells. It also discusses the role of fructose in overfeeding...

  • Multienzymatic Sucrose Conversion into Fructose and Gluconic Acid through Fed-Batch and Membrane-Continuous Processes. Taraboulsi, Fadi; Tomotani, Ester; Vitolo, Michele // Applied Biochemistry & Biotechnology;Dec2011, Vol. 165 Issue 7/8, p1708 

    Multienzymatic conversion of sucrose into fructose and gluconic acid was studied through fed-batch and continuous (in a membrane reactor) processes. The law of substrate addition (sucrose or glucose) for the fed-batch process which led to a yield superior to 80% was the decreasing linear type,...

  • Forty-two year survival with bilateral Judet hip prostheses. Tatari, Hasan; Alici, Emin; Havitçio&gcaron;lu, Hasan // Archives of Orthopaedic & Trauma Surgery;Jan2001, Vol. 121 Issue 1/2, p112 

    In this case report, a 70-year-old man is presented who had begun to suffer from ankylosing spondylitis when he was 12 years old and had been operated on because of bilateral hip ankylosis at the age of 21 years. The interesting aspect of this case is that both hips were replaced with Judet type...

  • Use of the mannitol pathway in fructose fermentation of Oenococcus oeni due to limiting redox regeneration capacity of the ethanol pathway. Richter, Hanno; Hamann, Inka; Unden, Gottfried // Archives of Microbiology;Apr2003, Vol. 179 Issue 4, p227 

    The heterolactic bacterium Oenococcus oeni ferments fructose by a mixed heterolactic/mannitol fermentation. For heterolactic fermentation of fructose, the phosphoketolase pathway is used. The excess NAD(P)H from the phosphoketolase pathway is reoxidized by fructose (yielding mannitol). It is...

  • Fructose-rich diet leads to reduced aerobic capacity and to liver injury in rats. Diego Botezelli, Diego Botezelli; Cambri, Lucieli Teresa; Ghezzi, Ana; Augusto Dalia, Rodrigo; Azevedo Voltarelli, Fabr�cio; Rostom de Mello, Maria Alice // Lipids in Health & Disease;2012, Vol. 11 Issue 1, p78 

    The main purpose of this research was to investigate the alterations in the aerobic capacity and appearance of metabolic alterations in Wistar rats fed on fructose-rich diet. We separated twenty-eight rats into two groups according to diet: a control group (C) (balanced diet) and a fructose-rich...

  • Amerind Ancestry, Socioeconomic Status and the Genetics of Type 2 Diabetes in a Colombian Population. Campbell, Desmond D.; Parra, Maria V.; Duque, Constanza; Gallego, Natalia; Franco, Liliana; Tandon, Arti; Hünemeier, Tábita; Bortolini, Cátira; Villegas, Alberto; Bedoya, Gabriel; McCarthy, Mark I.; Price, Alkes; Reich, David; Ruiz-Linares, Andrés // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    The "thrifty genotype" hypothesis proposes that the high prevalence of type 2 diabetes (T2D) in Native Americans and admixed Latin Americans has a genetic basis and reflects an evolutionary adaptation to a past low calorie/high exercise lifestyle. However, identification of the gene variants...

  • Protection of Rat Cardiac Myocytes by Fructose-1,6- Bisphosphate and 2,3-Butanedione. Wheeler, Thomas J.; Chien, Sufan // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    Earlier studies by our group showed that fructose-1,6-bisphosphate (FBP) enhances the hypothermic preservation of rat cardiac myocytes and the functional recovery of animal hearts after hypothermic storage. However, the mechanisms involved were not clear. We extended the cardiomyocyte studies by...

  • Interaction Between Some Monosaccharides and Aspartic Acid in Dilute Aqueous Solutions. Kulikova, Galina A.; Parfenyuk, Elena V. // Journal of Biological Physics;Jul2007, Vol. 33 Issue 3, p247 

    Interaction between aspartic acid and d-glucose, d-galactose, and d-fructose has been studied by isothermal titration calorimetry, calorimetry of dissolution, and densimetry. It has been found that d-glucose and d-fructose form thermodynamically stable associates with aspartic acid, in contrast...

  • The nature of autocatalysis in the Butlerov reaction. Simonov, A. N.; Pestunova, O. P.; Matvienko, L. G.; Parmon, V. N. // Kinetics & Catalysis;Mar2007, Vol. 48 Issue 2, p245 

    The effect of the nature of an initiator on the kinetics of formaldehyde consumption and on product composition in the Butlerov reaction was studied in a stirred flow reactor and a batch reactor. It was found that, under flow conditions, the kinetics and the product composition of this reaction...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics