TITLE

Overexpression of ZEB1 associated with metastasis and invasion in patients with gastric carcinoma

AUTHOR(S)
Jia, Baoqing; Liu, Hongyi; Kong, Qinglong; Li, Bing
PUB. DATE
July 2012
SOURCE
Molecular & Cellular Biochemistry;Jul2012, Vol. 366 Issue 1/2, p223
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The aim of this study was to investigate the expression of ZEB1 in gastric carcinoma, its correlation with the clinicopathology of gastric carcinoma, and the role of ZEB1 in invasion and metastasis in gastric carcinoma. ZEB1 expression was analyzed by immunohistochemistry and Western blot in 45 gastric carcinoma tissue samples that contained the adjacent gastric mucosa. The correlation between ZEB1 expression, the occurrence and development of gastric cancer, and clinical pathology was investigated. ZEB1 expression in the human gastric carcinoma cell line AGS was downregulated by RNA interference, and changes in ZEB1 expression corresponded with changes in the invasive and metastatic ability of AGS cells. Immunohistochemistry revealed that ZEB1 protein expression in gastric carcinoma tissues was significantly higher than in normal gastric mucosa tissues ( p < 0.001). A lower degree of differentiation of gastric cancer ( p = 0.009), a higher TNM (tumor, node, and metastasis) stage ( p = 0.010), and a larger scope of invasion were correlated with higher expression of ZEB1 ( p = 0.041, 0.002). However, the expression of ZEB1 in gastric carcinoma tissue was independent of gender, age, and tumor size ( p > 0.05). Western blot results also showed that ZEB1 protein expression was significantly higher in gastric carcinoma tissue than in the adjacent normal gastric mucosa tissue ( p = 0.008). A lower degree of differentiation of the gastric carcinoma correlated with a higher TNM stage, and a larger scope of invasion correlated with increased ZEB1 expression ( p = 0.023). Transfection of ZEB1 siRNA in AGS cells significantly decreased the expression level of ZEB1 protein ( p = 0.035). Furthermore, the number of cells that could pass through the Transwell chamber was significantly lower in the transfected group than in the non-transfected control group ( p = 0.039), indicating that the suppression of ZEB1 expression could significantly reduce the invasive and metastatic ability of AGS cells ( p = 0.005). Concluding, in gastric carcinoma tissue, overexpression of ZEB1 may be related to the occurrence and development as well as invasion and metastasis of gastric carcinoma.
ACCESSION #
76350943

 

Related Articles

  • Comparison of alternate-day versus consecutive-day treatment with S-1: assessment of tumor growth inhibition and toxicity reduction in gastric cancer cell lines in vitro and in vivo. Arai, Wataru; Hosoya, Yoshinori; Haruta, Hidenori; Kurashina, Kentaro; Saito, Shin; Hirashima, Yuuki; Yokoyama, Taku; Zuiki, Toru; Sakuma, Kazuya; Hyodo, Masanobu; Yasuda, Yoshikazu; Nagai, Hideo; Shirasaka, Tetsuhiko // International Journal of Clinical Oncology;Dec2008, Vol. 13 Issue 6, p515 

    The toxic effects of S-1 can lead to discontinuation of treatment. Strategies for reducing toxicity without compromising therapeutic effectiveness are required. We used the human gastric cancer cell lines MKN28 and MKN45 to examine such strategies in vitro. The cell lines were treated with three...

  • High level of ezrin expression in colorectal cancer tissues is closely related to tumor malignancy. Hong-Jian Wang; Jin-Shui Zhu; Qiang Zhang; Qun Sun; Hua Guo; Servin, Alain L.; Braet, Filip // World Journal of Gastroenterology;4/28/2009, Vol. 15 Issue 16, p2016 

    AIM: To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis. METHODS: Eighty paraffin-embedded cancer tissue samples were selected from...

  • Overexpression of Grb2/HER2 signaling in Chinese gastric cancer: their relationship with clinicopathological parameters and prognostic significance. Guan Yu; Ying Chen; Jie Jun Wang // Journal of Cancer Research & Clinical Oncology;Oct2009, Vol. 135 Issue 10, p1331 

    Growth factor receptor-bound 2 (Grb2)-mediated HER2 signaling is thought to play a critical role in gastric cancer development, progression and metastasis. However, little is known about their expression in gastric cancer. In this study, we try to explore their relationship with...

  • Blockade of SDF-1/CXCR4 signalling inhibits pancreatic cancer progression in vitro via inactivation of canonical Wnt pathway. Wang, Z; Ma, Q; Liu, Q; Yu, H; Zhao, L; Shen, S; Yao, J // British Journal of Cancer;11/18/2008, Vol. 99 Issue 10, p1695 

    Extra-pancreatic metastasis is a difficult problem for surgical intervention in pancreatic cancer. CXC chemokine receptor 4 (CXCR4) was considered to have an important role in this process. We hypothesized it may contribute to the pancreatic cancer progression through influencing canonical Wnt...

  • Hepatic metastases of a gastrointestinal stromal tumour. Rane, Sharada R.; Bagwan, Izhar N.; Holla, Vasudeo V.; Joshi, Mukund M. // Indian Journal of Surgery;Jan/Feb2004, Vol. 66 Issue 1, p51 

    Gastrointestinal stromal tumours are rare visceral neoplasms. Some 3% to 38% of them have malignant histological features and are associated with a high incidence of recurrence. We present a patient with liver metastases from a malignant GIST.

  • Predictive factors for lymph node metastasis in poorly differentiated early gastric cancer and their impact on the surgical strategy. Hua Li; Ping Lu; Yang Lu; Cai-Gang Liu; Hui-Mian Xu; Shu-Bao Wang; Jun-Qing Chen // World Journal of Gastroenterology;7/14/2008, Vol. 14 Issue 26, p4222 

    AIM: To identify the predictive clinicopathological factors for lymph node metastasis (LNM) in poorly differentiated early gastric cancer (EGC) and to further expand the possibility of using endoscopic mucosal resection (EMR) for the treatment of poorly differentiated EGC. METHODS: Data were...

  • SOX2 in gastric carcinoma, but not Hath1, is related to patients' clinicopathological features and prognosis. Xin Zhang; Hao Yu; Yongtao Yang; Rong Zhu; Jianying Bai; Zhihong Peng; Yonghong He; Lei Chen; Wensheng Chen; Dianchun Fang; Xiuwu Bian; Rongquan Wang; Zhang, Xin; Yu, Hao; Yang, Yongtao; Zhu, Rong; Bai, Jianying; Peng, Zhihong; He, Yonghong; Chen, Lei // Journal of Gastrointestinal Surgery;Aug2010, Vol. 14 Issue 8, p1220 

    Background: SOX2 and Hath1 are transcription factors that are critical for the control of terminal cell differentiation in the gastrointestinal mucosa. This study investigated the correlations between SOX2 and Hath1 expression in gastric carcinoma and patients' clinicopathological...

  • RNA interference against MDM2 suppresses tumor growth and metastasis in pancreatic carcinoma SW1990HM cells. Shi, Weidong; Meng, Zhiqiang; Chen, Zhen; Hua, Yongqiang; Gao, Huifeng; Wang, Peng; Lin, Junhua; Zhou, Zhenhua; Luo, Jianmin; Liu, Luming // Molecular & Cellular Biochemistry;Feb2014, Vol. 387 Issue 1/2, p1 

    In our previous study, the mouse double minute 2 (MDM2) was identified as one of the leading genes that promote the metastasis of pancreatic cancer (PC). However, the mechanism by which MDM2 promotes metastasis of PC is not understood. In this study, we show that down-regulation of MDM2 through...

  • The Effects of Tamoxifen in Combination with Tranilast on CXCL12-CXCR4 Axis and Invasion in Breast Cancer Cell Lines. Darakhshan, Sara; Bidmeshkipour, Ali; Mansouri, Kamran; Saeid, Hakhamaneshi Mohammad; Ghanbari, Ali // Iranian Journal of Pharmaceutical Research;Spring2014, Vol. 13 Issue 2, p683 

    It has been reported that CXCL12 binding to CXCR4 induces several intracellular signaling pathways, and enhances survival, proliferation, and migration of malignant cells. In the present study, we examined the effects of anti-estrogen tamoxifen and anti-allergic tranilast drugs as a single or in...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics