TITLE

Determination of orientational order parameters of uniaxial films with a commercial 90°-angle fluorescence spectrometer

AUTHOR(S)
Damerau, T.; Hennecke, M.
PUB. DATE
October 1995
SOURCE
Journal of Chemical Physics;10/8/1995, Vol. 103 Issue 14, p6232
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A method is developed to determine orientational order parameters and of films by steady state fluorescence polarization in a spectrometer with 90° angle between excitation and emission beam. This is the geometry usually found in commercial fluorescence spectrometers. The method includes the determination of the fundamental molecular anisotropy rM of the fluorescent chromophore (doped or intrinsic) in stretched and unstretched films and the determination of instrumental correction coefficients from an isotropic film. As an example, the method is applied to orientation analysis of polyethylene and polystyrene films doped with fluorescent molecules of repetitive styrene units. It is concluded that in uniaxially stretched polyethylene-films orientation of these molecules is dominated by their molecular shape; in polystyrene films orientation is influenced by additional interactions. © 1995 American Institute of Physics.
ACCESSION #
7625001

 

Related Articles

  • Concentration depolarization of fluorescence in the presence of molecular rotation. Fredrickson, Glenn H. // Journal of Chemical Physics;5/1/1988, Vol. 88 Issue 9, p5291 

    A theory is presented to describe the observables in fluorescence depolarization experiments when both rotational depolarization and concentration depolarization mechanisms are active. The latter mechanism arises from electronic excitation migration among chromophores and becomes more important...

  • Energy migration and rotational motion within bichromophoric molecules. II. A derivation of the fluorescence anisotropy. Johansson, L. B.-Å.; Edman, Peter; Westlund, P.-O. // Journal of Chemical Physics;12/22/1996, Vol. 105 Issue 24, p10896 

    A generalized Förster theory is presented which includes reorientation of the interacting molecules. The stochastic master equation is, for the first time, derived from the stochastic Liouville equation, so that it accounts for the molecular origin to the stochastic transitions rates. A...

  • Collapse of single DNA molecule in poly(ethylene glycol) solutions. Vasilevskaya, V. V.; Khokhlov, A. R.; Matsuzawa, Y.; Yoshikawa, K. // Journal of Chemical Physics;4/22/1995, Vol. 102 Issue 16, p6595 

    The compactization of a single DNA molecule in polyethylene glycol (PEG) solution was investigated both theoretically and experimentally. A theory is proposed taking into account the polyelectrolyte effect and redistribution of PEG within DNA coils. This approach makes it possible to describe...

  • Nanoscale imaging of molecular positions and anisotropies. Gould, Travis J.; Gunewardene, Mudalige S.; Gudheti, Manasa V.; Verkhusha, Vladislav V.; Yin, Shu-Rong; Gosse, Julie A.; Hess, Samuel T. // Nature Methods;Dec2008, Vol. 5 Issue 12, p1027 

    Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution...

  • Spatial discrimination of Rydberg tagged molecular photofragments in an inhomogeneous electric field. Monti, O. L. A.; Cruse, H. A.; Softley, T. P.; Mackenzie, S. R. // Journal of Chemical Physics;11/1/2001, Vol. 115 Issue 17, p7924 

    A new approach to detecting the spatial and energetic distribution of photodissociation products is demonstrated, in which an inhomogeneous electric field is used for spatially selective field ionization of Rydberg molecules. State-selected NO fragments from NO[sub 2] photodissociation in a...

  • Effect of electroelastic anisotropy of DNA-like molecules on their tertiary structure. Golo, V. L.; Evdokimov, Yu. M.; Kats, E. I. // Journal of Experimental & Theoretical Physics;Dec97, Vol. 85 Issue 6, p1180 

    Under certain conditions, mechanical forces can cause an anisotropic molecule like DNA to assume a toroidal spatial structure. A simple model describing such a behavior is suggested. The model incorporates anisotropic elastic energy and external electrical forces. The steadystate structures...

  • Solubilities of small molecules in polyethylene evaluated by a test-particle-insertion method. Fukuda, Mitsuhiro // Journal of Chemical Physics;1/1/2000, Vol. 112 Issue 1 

    Free-energy related properties for penetrant sorption (water, methane, argon, oxygen, nitrogen, ethane, propane, and carbon dioxide) in amorphous polyethylene have been evaluated by a test-particle-insertion method with the excluded volume map sampling (EVMS). Two model systems, one a long chain...

  • Detection and lifetime measurement of single molecules in flowing sample streams by.... Wilkerson Jr., Charles W.; Goodwin, Peter M.; Ambrose, W. Patrick; Martin, John C.; Keller, Richard A. // Applied Physics Letters;4/26/1993, Vol. 62 Issue 17, p2030 

    Demonstrates detection and measurement of fluorescence lifetimes of single molecules in flowing sample streams. Use of time-correlated single-photon counting for detection; Importance of Raman solvent and Rayleigh backgrounds; Estimation of detected molecules.

  • Exponential and logarithmic spectral diffusion in single molecule fluorescence. Osad'ko, I. S.; Yershova, L. B. // Journal of Chemical Physics;6/1/2000, Vol. 112 Issue 21, p9645 

    A dynamical theory for two-photon autocorrelation functions (AF) which takes into account interaction of a chromophore with phonons and with huge number of tunneling systems (TSs) of a cooled polymer is developed. The interaction with one extrinsic TS, which relates to the chromophore with its...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics