Polymorphism of the beta 3-adrenergic receptor gene (ADRB3) and its distribution in domestic and wild pigs in China

Jianliang Wu; Jianhua Liu; Yanan Yuan; Liying Qiao; Haibo Hao; Wenzhong Liu
April 2012
Animal Science Papers & Reports;2012, Vol. 30 Issue 2, p139
Academic Journal
The beta 3-adrenergic receptor (ADRB3) is a G-protein coupled receptor encoded by ADRB3 gene and involved in regulation of lipolysis and homeostasis. The objective of this study was to detect the genetic variation existed in this gene in eight domestic and wild pig populations. The polymorphism of partial sequences of ADRB3 gene in 336 pigs was analysed by single strand conformation polymorphism technique based on PCR (PCR-SSCP). Fst statistic and analysis of molecular variance (AMOVA) were used to measure the genetic differentiation. The genetic distances between populations were computed and a phylogenic tree was reconstructed by the unweighted pair group method using the arithmetic mean. Five haplotypes named A, B, C, D and E were detected with the frequencies ranging from 0.060 for E to 0.324 for A. The reliability of all haplotype frequencies reached 0.95. The haplotypes involved four polymorphic sites, of which one located in exon 2 with a thymine insertion, and the remaining three in non-coding regions with G/A and G/T substitutions, respectively. The thymine insertion resulted in the absence of two amino acids in the sequence of receptor protein. The haplotype diversity ranged from 0.579±0.057 in pure wild Liaoning to 0.792±0.024 in Mashen pigs, indicating abundant genetic diversity to exist in the populations. The fist estimates showed that the degree of population differentiation between domestic and wild pigs was moderate, but at a lower level within domestic populations. About 94.38% of the genetic variation originated from within the populations by AMOVA.


Related Articles

  • Relationship between ß-2 adrenoceptor gene haplotypes and adipocyte lipolysis in women. Eriksson, P; Dahlman, I; Rydén, M; Hoffstedt, J; Arner, P // International Journal of Obesity & Related Metabolic Disorders;Feb2004, Vol. 28 Issue 2, p185 

    BACKGROUND AND AIMS:: The ß2-adrenergic receptors are important for adipocyte lipolysis regulation by catecholamines in humans. The ß2-adrenoceptor gene is highly polymorphic. The role of these genetic variations for adipocyte lipolysis was investigated. DESIGN AND METHODS:: Six...

  • Polymorphism of the H-FABP, MC4R and ADD1 genes in the Meishan and four other pig populations in China. Li, C. L.; Pan, Y. C.; Meng, H. // South African Journal of Animal Science;2006, Vol. 36 Issue 1, p1 

    Genes such as the Heart Fatty Acid-Binding Protein (H-FABP), the Melanocortin-4 Receptor (MC4R) and the Adipocyte Determination and Differentiation factor-1 (ADD1) play an important role in meat quality in pigs. The Meishan is one of the most prolific pig breeds in the world, but it is in...

  • Spinophilin is required for normal morphology, Ca homeostasis and contraction but dispensable for β-adrenergic stimulation of adult cardiomyocytes. Petzhold, Daria; Costa-Goncalves, André; Gross, Volkmar; Morano, Ingo // Journal of Muscle Research & Cell Motility;Dec2011, Vol. 32 Issue 4/5, p243 

    Spinophilin (SPN) is a ubiquitously expressed scaffolding protein that interacts through several binding modules with a variety of target proteins. Thus, SPN bundles F-actin, targets protein phosphatase 1 to the ryanodine receptor, and targets regulators of G-protein signaling to G-protein...

  • Enhanced β2-adrenergic receptor (β2AR) signaling by adeno-associated viral (AAV)-mediated gene transfer. Jones, Stacie M.; Hiller, F. Charles; Jacobi, Sandie E.; Foreman, Susan K.; Pittman, Laura M.; Cornett, Lawrence E. // BMC Pharmacology;2003, Vol. 3, p1 

    Background: β2-Adrenergic receptors (β2AR) play important regulatory roles in a variety of cells and organ systems and are important therapeutic targets in the treatment of airway and cardiovascular disease. Prolonged use of β-agonists results in tolerance secondary to receptor...

  • Impaired β-adrenergic signaling pathway in white adipocytes of suckling fa/fa Zucker rats: a defect in receptor coupling. Mory, G; Wiel, M; Adli, H; Diot-Dupuy, F; Ferré, P; Bazin, R // International Journal of Obesity & Related Metabolic Disorders;Nov2001, Vol. 25 Issue 11, p1592 

    BACKGROUND: In fa/fa Zucker rats, leptin receptor deficiency is responsible for both a deficit of energy expenditure and hyperphagia which lead to massive obesity and insulin resistance in adulthood. This obesity is also characterised by alterations of the β-adrenergic signaling pathway....

  • Cardiac natriuretic peptides act via p38 MAPK to induce the brown fat thermogenic program in mouse and human adipocytes. Bordicchia, Marica; Dianxin Liu; Amri, Ez-Zoubir; Ailhaud, Gerard; Dessì-Fulgheri, Paolo; Chaoying Zhang; Takahashi, Nobuyuki; Sarzani, Riccardo; Collins, Sheila // Journal of Clinical Investigation;Mar2012, Vol. 122 Issue 3, p1022 

    The ability of mammals to resist body fat accumulation is linked to their ability to expand the number and activity of "brown adipocytes" within white fat depots. Activation of β-adrenergic receptors (β-ARs) can induce a functional "brown-like" adipocyte phenotype. As cardiac natriuretic...

  • Expression variation of the porcine ADRB2 has a complex genetic background. Murani, Eduard; Ponsuksili, Siriluck; Reyer, Henry; Wittenburg, Dörte; Wimmers, Klaus // Molecular Genetics & Genomics;Nov2013, Vol. 288 Issue 11, p615 

    Porcine adrenergic receptor beta 2 (ADRB2) gene exhibits differential allelic expression in skeletal muscle, and its genetic variation has been associated with muscle pH. Exploring the molecular-genetic background of expression variation for porcine ADRB2 will provide insight into the mechanisms...

  • Structural biology: A receptor unlocked. Sprang, Stephen R. // Nature;11/15/2007, Vol. 450 Issue 7168, p355 

    The article explores the structure β2-adrenergic receptors (β2AR), which are archetypal members of G-protein-coupled receptors (GPCR), and the molecular basis of their activity in many biological functions. The author inferred that GPCRs activate the eponymous G proteins which are attached...

  • TotalβAdrenoceptor Deficiency Results in Cardiac Hypotrophy and Negative Inotropy.  // Physiological Research;2010, Vol. 59 Issue 5, p679 

    No abstract available.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics