TITLE

Responses of Arma chinensis cold tolerance to rapid cold hardening and underlying physiological mechanisms

AUTHOR(S)
LI Xing-peng; SONG Li-wen; ZHANG Hong-hao; CHEN Yue-qu; ZUO Tong-tong; WANG Jun; SUN Wei
PUB. DATE
March 2012
SOURCE
Yingyong Shengtai Xuebao;Mar2012, Vol. 23 Issue 3, p791
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Rapid cold hardening can enhance the cold tolerance of some insects. To explore the effects of different cold hardening induction temperature on the cold tolerance of Arma chinensis and related physiological mechanisms, the 3rd generation A. chinensis adults reared indoor were treated with cooling at 15, 10, and 4 °C for 4 h, respectively, or with gradual cooling from 15 °C for 4 h to 10 °C for 4 h, and finally to 4 °C for 4 h. The super-cooling point, water content, and the contents of low molecular carbohydrates, glycerol, and amino acids of the adults after cooling and the adults cold tolerance at 0, -5, and -10 °Cwere measured by thermocouple, high performance liquid chromatography, and other analytical techniques. When exposed at -10 °C after cooling, the sur- vival rate of the adults treated with gradual cooling or treated with cooling at 4 °C for 4 h was aver- agely 58. 3%, while that of the adults reared at room temperature (25 °C±2 °C) or treated with cooling at 15 °Cor 10 °C for 4 h decreased significantly, with an average of 8. 9%. The super-cool- ing point of the adults treated with gradual cooling or with cooling at 4 °C for 4 h was -15. 6 °C, which was averagely 1. 3 °Clower than that of the other treatments. The water content of the adults had no significant difference among all treatments, with an average of 61. 8%, but the glucose, sor- bitolum, glycerol, Ala, and Glu contents in treatments gradual cooling and cooling at 4 °C for 4 h increased by 2. 82-fold, 2. 65-fold, 3. 49-fold, 51. 3%, and 80. 2%, while the fucose, mannose, and Pro contents decreased by 68. 4%, 52. 2%, and 30. 2%, respectively, as compared with the other treatments. The fructose content showed no significant difference among all treatments. It was suggested that rapid cool hardening had a critical temperature to induce the physiological metabolism process of adult A. chinensis, and gradual cooling hardening could not further increase the cold tol- erance of adult A. chinensis on the basis of rapid cool hardening.
ACCESSION #
76115136

 

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