Gene expression of the key enzymes controlling starch synthesis and metabolism in rice grain endosperm under effects of high temperature after anthesis

ZHONG Lian-jin; DONG Hu; CAI Xiao-bo; FENG Yan-ning; REN Ping; CHENG Fang-min
March 2012
Yingyong Shengtai Xuebao;Mar2012, Vol. 23 Issue 3, p745
Academic Journal
Taking an early-season indica cultivar 'Jiazao 935'whose grain quality was sensitive to temperature as test material, and by using artificial climatic chamber and real-time fluorescence quantitative PCR (FQ-PCR), this paper studied the relative expression amount and its dynamic changes of ten isoform genes of the key enzymes controlling starch synthesis and metabolism in rice grain endosperm, including sbe1, sbe3, and sbe4 of starch branching enzyme (SBE), isa1, isa2, isa3, and pul of starch debranching enzyme (DBE), and Wx, sss1, and sss2a of starch synthase (SS), at the mean daily temperature 22 and 32°C after anthesis. There existed obvious differences in the expression patterns of these genes under the high temperature stress, and the expression pat- terns were isoform-dependent. The relative expression amount of sbe1 and sbe3 under high tempera- ture decreased significantly, and both of the genes were the sensitive isoform genes of SBE to high temperature stress. Among the DBE genes, pul was the isoform gene with high expression level, be- ing more sensitive to high temperature stress than isa1, isa2, and isa3. Among the SS genes, sss2a had a significantly lower relative expression amount than sss1 and Wx, but sss2a and sss1 were more sensitive to high temperature than Wx, suggesting that sss2a and sss1 could be the important genes that adjusted the starch structure in rice endosperm under high temperature stress, especially at the middle and late grain filling stages.


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