Sperm gene expression profile is related to pregnancy rate after insemination and is predictive of low fecundity in normozoospermic men

Bonache, Sandra; Mata, Ana; Ramos, María Dolores; Bassas, Lluís; Larriba, Sara
June 2012
Human Reproduction;Jun2012, Vol. 27 Issue 6, p1556
Academic Journal
BACKGROUND Assessment of male fertility is traditionally based on microscopic evaluation of semen. However, the classical semen parameters do not adequately reflect sperm function, and their clinical value in predicting fertility is limited. We hypothesize that the sperm expression profile could reflect the fertilizing quality of spermatozoa and could be more informative for predicting the in vivo reproductive fitness of men with normal semen parameters. METHODS Sperm gene expression patterns of 68 normozoospermic donors (43 Phase I and 25 Phase II), used for therapeutic IUI, were analysed via TaqMan Arrays. RESULTS Significant differences in the expression of individual genes were observed between groups of donors with the lowest and highest pregnancy rates (PRs) after IUI. Additionally, we have developed a molecular means to classify the fertility status of semen donors for IUI based on the expression signature of four genes. In the Phase I study, this model had 90% sensitivity and 97% specificity for discriminating donors resulting in low PRs (cut-off value: <13.6%), far better than that obtained from the combination of sperm parameters. The translation of the model was validated in Phase II donors resulting in a sensitivity of 71.5% and a specificity of 78%. CONCLUSIONS Our findings contribute to the search for the most valuable genetic markers which are potentially useful as tools for predicting pregnancy. Our expression model could complement classical semen analysis in order to identify sperm donors with a less favourable IUI reproductive outcome despite having normal semen parameters. It may also be useful for the study of sperm function in couples with unexplained infertility.


Related Articles

  • Single vs. Double Insemination for Male Factor and Idiopathic Infertility. Guangxiu Lu // Fertility Weekly;7/24/2006-7/31/2006, p7 

    The article presents a study which compares the effectiveness of double intrauterine insemination (IUI) with single IUI in male factor and idiopathic infertility patients. The study revealed that double IUI increases the pregnancy rate in patients in male factor infertility while single IUI...

  • Successful treatment of infertility due to retrograde ejaculation. Scammell, G. E. // Journal of the Royal Society of Medicine;Dec1981, Vol. 74 Issue 12, p926 

    The article focuses on the case report on the initiation of human serum albumin (HSA) to resolve infertility in Great Britain. Successful pregnancy in this new technique whereby the sperm is resuspended in 4 percent HSA is assessed. The study is undertaken to assure the validity of the method,...

  • Des hommes médicalement assistés pour procréer. de LA ROCHEBROCHARD, Élise // Population (00324663);jui-oct2003, Vol. 58 Issue 4/5, p549 

    In response to involuntary infertility, which affects 15% to 20% of couples, the techniques of in vitro fertilization (IVF) have been developed. Between 1982 and 2000, 85,000 children were born in France thanks to IVF (estimates based on combined analysis of the FIVNAT survey and administrative...

  • Other options for male infertility.  // GP: General Practitioner;4/8/2002, p64 

    Examines the role of intracytoplasmic sperm injection in the management of male infertility in Great Britain. Effectiveness of donor insemination on men with inherited disorder; Aim of the treatment to achieve the production of oocytes in conjunction with intrauterine insemination of prepared...

  • SELECTED ORAL COMMUNICATION SESSION, SESSION 70: ANDROLOGY AND SEMINAL FACTORS Wednesday 6 July 2011 14:00 - 15:45.  // Human Reproduction;Jan2011 Supplement 1, Vol. 26 Issue suppl_1, pi110 

    No abstract available.

  • Effects of Main Semen Parameters on Intrauterian Insemination (IUI) Outcome. Aboutorabi, Roshanak; Dayani, Mahbobeh; Dastgerdi, Mehdi Nikbakht // Journal of Isfahan Medical School;Dec2010, Vol. 28 Issue 112, p1 

    Background: There are several techniques to manage sub fertility through which, Intrauterian Insemination (IUI) is on first step treatment strategy due to its simplicity and low costing and also being non invasive. The outcome of IUI is dependent on main semen parameters such as, sperm...

  • Antibiotics supplemented culture media. Tin, Sim Sai; Wiwanitkit, Viroj // Journal of Human Reproductive Sciences;Jul-Sep2014, Vol. 7 Issue 3, p227 

    No abstract available.

  • Conference Abstracts . . . October 4, 2010.  // Fertility Weekly;10/4/2010, p13 

    The article presents abstracts related to human fertility and reproductive science which include the birth following intracytoplasmic sperm injection, the correlation of sperm DNA decondensation with sperm morphological anomalies and sperm motility, and the reproductive results using Kruger's...

  • Timing of ICSI and ROSI and Efficient Offspring Production.  // Fertility Weekly;7/5/2004, p8 

    Reports on a study which found that spermatozoa and round spermatids have distinct potentials for establishing the formation of a male pronucleus in activated oocytes. Evaluation on the influence of timing intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) on the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics